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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

AGAP004752

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei136S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei139UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:AGAP004752
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 2L, Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705651 – 235tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST235

Proteomic databases

PaxDbiQ7Q2P7
PRIDEiQ7Q2P7

Interactioni

Protein-protein interaction databases

STRINGi7165.AGAP004752-PA

Structurei

3D structure databases

ProteinModelPortaliQ7Q2P7
SMRiQ7Q2P7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni83 – 84S-adenosyl-L-methionine bindingUniRule annotation2
Regioni116 – 117S-adenosyl-L-methionine bindingUniRule annotation2
Regioni214 – 216S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
HOGENOMiHOG000260965
InParanoidiQ7Q2P7
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG091G0IQ7
PhylomeDBiQ7Q2P7

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q7Q2P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEAIDDHVK LPQKRFYRQR AHSNPIADHS FDYPLLPELS NWNELYPNIG
60 70 80 90 100
NRQVVFADIG CGYGGFLVTL GETFPDKLAI GMEIRVKVSD YVMDRIKALR
110 120 130 140 150
QRHKGKYENI ACIRTNAMKY LPNYFRKHQL EKLFFLYPDP HFKKAKHKWR
160 170 180 190 200
IINPTLLSEY AYVLRPGGKI YTVTDVRELH EWMCKHIEEH PCFKRLPDTE
210 220 230
AQEDILAPKL LDSSEEGQKV TRMSGEKFMA IFTRL
Length:235
Mass (Da):27,662
Last modified:October 23, 2007 - v4
Checksum:iCBEFA9F6979C7819
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAAB01008968 Genomic DNA Translation: EAA13331.4
RefSeqiXP_318065.4, XM_318065.4

Genome annotation databases

EnsemblMetazoaiAGAP004752-RA; AGAP004752-PA; AGAP004752
GeneIDi1278473
KEGGiaga:AgaP_AGAP004752
VectorBaseiAGAP004752-RA; AGAP004752-PA; AGAP004752

Similar proteinsi

Entry informationi

Entry nameiTRMB_ANOGA
AccessioniPrimary (citable) accession number: Q7Q2P7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 23, 2007
Last modified: May 23, 2018
This is version 95 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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