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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

AGAP004752

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei136S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei139UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:AGAP004752
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 2L, Unassembled WGS sequence

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705651 – 235tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST235

Proteomic databases

PaxDbiQ7Q2P7.

Interactioni

Protein-protein interaction databases

STRINGi7165.AGAP004752-PA.

Structurei

3D structure databases

ProteinModelPortaliQ7Q2P7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni83 – 84S-adenosyl-L-methionine bindingUniRule annotation2
Regioni116 – 117S-adenosyl-L-methionine bindingUniRule annotation2
Regioni214 – 216S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
InParanoidiQ7Q2P7.
KOiK03439.
OMAiKWRIINQ.
OrthoDBiEOG0917077G.
PhylomeDBiQ7Q2P7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7Q2P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEAIDDHVK LPQKRFYRQR AHSNPIADHS FDYPLLPELS NWNELYPNIG
60 70 80 90 100
NRQVVFADIG CGYGGFLVTL GETFPDKLAI GMEIRVKVSD YVMDRIKALR
110 120 130 140 150
QRHKGKYENI ACIRTNAMKY LPNYFRKHQL EKLFFLYPDP HFKKAKHKWR
160 170 180 190 200
IINPTLLSEY AYVLRPGGKI YTVTDVRELH EWMCKHIEEH PCFKRLPDTE
210 220 230
AQEDILAPKL LDSSEEGQKV TRMSGEKFMA IFTRL
Length:235
Mass (Da):27,662
Last modified:October 23, 2007 - v4
Checksum:iCBEFA9F6979C7819
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAAB01008968 Genomic DNA. Translation: EAA13331.4.
RefSeqiXP_318065.4. XM_318065.4.

Genome annotation databases

EnsemblMetazoaiAGAP004752-RA; AGAP004752-PA; AGAP004752.
GeneIDi1278473.
KEGGiaga:AgaP_AGAP004752.
VectorBaseiAGAP004752-RA; AGAP004752-PA; AGAP004752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAAB01008968 Genomic DNA. Translation: EAA13331.4.
RefSeqiXP_318065.4. XM_318065.4.

3D structure databases

ProteinModelPortaliQ7Q2P7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7165.AGAP004752-PA.

Proteomic databases

PaxDbiQ7Q2P7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP004752-RA; AGAP004752-PA; AGAP004752.
GeneIDi1278473.
KEGGiaga:AgaP_AGAP004752.
VectorBaseiAGAP004752-RA; AGAP004752-PA; AGAP004752.

Organism-specific databases

CTDi1278473.

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
InParanoidiQ7Q2P7.
KOiK03439.
OMAiKWRIINQ.
OrthoDBiEOG0917077G.
PhylomeDBiQ7Q2P7.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_ANOGA
AccessioniPrimary (citable) accession number: Q7Q2P7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 23, 2007
Last modified: February 15, 2017
This is version 88 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.