Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Argininosuccinate synthase

Gene

AGAP003015

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (AGAP003015)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline.
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate synthase (AGAP003015)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei86CitrullineBy similarity1
Binding sitei91CitrullineBy similarity1
Binding sitei118AspartateBy similarity1
Binding sitei122AspartateBy similarity1
Binding sitei122CitrullineBy similarity1
Binding sitei123AspartateBy similarity1
Binding sitei126CitrullineBy similarity1
Binding sitei179CitrullineBy similarity1
Binding sitei188CitrullineBy similarity1
Binding sitei269CitrullineBy similarity1
Binding sitei281CitrullineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 17ATPBy similarity9
Nucleotide bindingi114 – 122ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
ORF Names:AGAP003015
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
Proteomesi
  • UP000007062 Componentsi: Chromosome 2R, Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003213271 – 424Argininosuccinate synthaseAdd BLAST424

Proteomic databases

PaxDbiQ7PR38.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7165.AGAP003015-PA.

Structurei

3D structure databases

ProteinModelPortaliQ7PR38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family.Curated

Phylogenomic databases

eggNOGiKOG1706. Eukaryota.
COG0137. LUCA.
HOGENOMiHOG000230093.
InParanoidiQ7PR38.
KOiK01940.
OMAiEVICFMA.
OrthoDBiEOG091G0AIR.
PhylomeDBiQ7PR38.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7PR38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKEKVLLAY SGGLDTSCIL KWLLEQDYEV ICFMADVGQT EDFAAAREKA
60 70 80 90 100
LKIGAADVVV KDMKDFFVEK FVWPAVQMGL IYEDRYLLGT SLARPCISKG
110 120 130 140 150
LMDVAVQRGC SYISHGATGK GNDQIRFELS CYALSPTIRV IAPWRMETFC
160 170 180 190 200
QRFQGRSDLL EYAKRSNIPV SATTKAPWSM DANIMHISYE SGILEDPSVA
210 220 230 240 250
APEELYQLTQ SPTRAPDTPT VAEIVFKAGL PVRVTELVSG RTMERPVDIL
260 270 280 290 300
AFLNKAGGEH GVGRIDIVEN RYIGLKSRGV YETPGVTVLH CAHRDLEIYC
310 320 330 340 350
LDREVLRVKK YLADRMADYV YNGYWYAPEA EYVRKCILES QKHVSGKVVV
360 370 380 390 400
EMFKGHVMVT SRESMHSLYN QELASMEVHG NFSPYSSTGF IEINAVRLRE
410 420
HHRVFGTANM TKHFSRTESD MKLI
Length:424
Mass (Da):47,807
Last modified:January 9, 2007 - v3
Checksum:i133485FD4A9CC4B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAAB01008859 Genomic DNA. Translation: EAA07894.4.
RefSeqiXP_311863.3. XM_311863.4.

Genome annotation databases

EnsemblMetazoaiAGAP003015-RA; AGAP003015-PA; AGAP003015.
GeneIDi1272938.
KEGGiaga:AgaP_AGAP003015.
VectorBaseiAGAP003015-RA; AGAP003015-PA; AGAP003015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAAB01008859 Genomic DNA. Translation: EAA07894.4.
RefSeqiXP_311863.3. XM_311863.4.

3D structure databases

ProteinModelPortaliQ7PR38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7165.AGAP003015-PA.

Proteomic databases

PaxDbiQ7PR38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP003015-RA; AGAP003015-PA; AGAP003015.
GeneIDi1272938.
KEGGiaga:AgaP_AGAP003015.
VectorBaseiAGAP003015-RA; AGAP003015-PA; AGAP003015.

Organism-specific databases

CTDi1272938.

Phylogenomic databases

eggNOGiKOG1706. Eukaryota.
COG0137. LUCA.
HOGENOMiHOG000230093.
InParanoidiQ7PR38.
KOiK01940.
OMAiEVICFMA.
OrthoDBiEOG091G0AIR.
PhylomeDBiQ7PR38.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_ANOGA
AccessioniPrimary (citable) accession number: Q7PR38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 9, 2007
Last modified: November 30, 2016
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.