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Protein

Argininosuccinate synthase

Gene

AGAP003015

Organism
Anopheles gambiae (African malaria mosquito)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (AGAP003015)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathway: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline.
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate synthase (AGAP003015)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei86 – 861CitrullineBy similarity
Binding sitei91 – 911CitrullineBy similarity
Binding sitei118 – 1181AspartateBy similarity
Binding sitei122 – 1221AspartateBy similarity
Binding sitei122 – 1221CitrullineBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei126 – 1261CitrullineBy similarity
Binding sitei179 – 1791CitrullineBy similarity
Binding sitei188 – 1881CitrullineBy similarity
Binding sitei269 – 2691CitrullineBy similarity
Binding sitei281 – 2811CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 179ATPBy similarity
Nucleotide bindingi114 – 1229ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
ORF Names:AGAP003015
OrganismiAnopheles gambiae (African malaria mosquito)
Taxonomic identifieri7165 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeAnophelinaeAnopheles
ProteomesiUP000007062 Componenti: Chromosome 2R

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Argininosuccinate synthasePRO_0000321327Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7165.AGAP003015-PA.

Structurei

3D structure databases

ProteinModelPortaliQ7PR38.
SMRiQ7PR38. Positions 3-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiQ7PR38.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG7PVWPB.
PhylomeDBiQ7PR38.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7PR38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKEKVLLAY SGGLDTSCIL KWLLEQDYEV ICFMADVGQT EDFAAAREKA
60 70 80 90 100
LKIGAADVVV KDMKDFFVEK FVWPAVQMGL IYEDRYLLGT SLARPCISKG
110 120 130 140 150
LMDVAVQRGC SYISHGATGK GNDQIRFELS CYALSPTIRV IAPWRMETFC
160 170 180 190 200
QRFQGRSDLL EYAKRSNIPV SATTKAPWSM DANIMHISYE SGILEDPSVA
210 220 230 240 250
APEELYQLTQ SPTRAPDTPT VAEIVFKAGL PVRVTELVSG RTMERPVDIL
260 270 280 290 300
AFLNKAGGEH GVGRIDIVEN RYIGLKSRGV YETPGVTVLH CAHRDLEIYC
310 320 330 340 350
LDREVLRVKK YLADRMADYV YNGYWYAPEA EYVRKCILES QKHVSGKVVV
360 370 380 390 400
EMFKGHVMVT SRESMHSLYN QELASMEVHG NFSPYSSTGF IEINAVRLRE
410 420
HHRVFGTANM TKHFSRTESD MKLI
Length:424
Mass (Da):47,807
Last modified:January 9, 2007 - v3
Checksum:i133485FD4A9CC4B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAAB01008859 Genomic DNA. Translation: EAA07894.4.
RefSeqiXP_311863.3. XM_311863.4.

Genome annotation databases

EnsemblMetazoaiAGAP003015-RA; AGAP003015-PA; AGAP003015.
GeneIDi1272938.
KEGGiaga:AgaP_AGAP003015.
VectorBaseiAGAP003015. Anopheles gambiae.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAAB01008859 Genomic DNA. Translation: EAA07894.4.
RefSeqiXP_311863.3. XM_311863.4.

3D structure databases

ProteinModelPortaliQ7PR38.
SMRiQ7PR38. Positions 3-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7165.AGAP003015-PA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAGAP003015-RA; AGAP003015-PA; AGAP003015.
GeneIDi1272938.
KEGGiaga:AgaP_AGAP003015.
VectorBaseiAGAP003015. Anopheles gambiae.

Organism-specific databases

CTDi1272938.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiQ7PR38.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG7PVWPB.
PhylomeDBiQ7PR38.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the malaria mosquito Anopheles gambiae."
    Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F.
    , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L.
    Science 298:129-149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PEST.

Entry informationi

Entry nameiASSY_ANOGA
AccessioniPrimary (citable) accession number: Q7PR38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 9, 2007
Last modified: January 7, 2015
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.