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Protein

Protein argonaute-3

Gene

AGO3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts via the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates predominantly with sense piRNAs that contain adenine at nucleotide 10, but shows no preference for uridine at the 5' end. In testis, however, associates with Su(Ste) and AT-chX-1 piRNAs mostly produced from antisense precursors. Shows RNA cleavage activity. In the germline, acts to amplify pools of antisense piRNAs, among others Su(Ste), AT-chX-1 and roo, and to limit sense piRNA accumulation. Forms a complex with smg, twin, aub and specific piRNAs that targets nos mRNA (and probably other maternal mRNAS) for deadenylation promoting its decay during early embryogenesis. Involved in transposon silencing in the adult brain.6 Publications

GO - Molecular functioni

  • piRNA binding Source: FlyBase
  • RNA binding Source: FlyBase

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of transposition Source: FlyBase
  • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: FlyBase
  • posttranscriptional gene silencing by RNA Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein argonaute-3Imported
Gene namesi
Name:AGO3
ORF Names:CG40300
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0250816. AGO3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: FlyBase
  • mitochondrion Source: FlyBase
  • P granule Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Female sterility. Male semi-sterility accompanied by production of Ste protein crystals in primary spermatocytes. Mutant males fail to maintain germline stem cells.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi68 – 72RGRAR → KGKAK: Abolishes binding to papi. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004229141 – 867Protein argonaute-3Add BLAST867

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4Symmetric dimethylarginine1 Publication1
Modified residuei68Symmetric dimethylarginine1 Publication1
Modified residuei70Symmetric dimethylarginine1 Publication1

Post-translational modificationi

Symmetrically dimethylated on Arg-4, Arg-68 and Arg-70, most likely by csul.1 Publication2 Publications

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ7PLK0.
PRIDEiQ7PLK0.

Expressioni

Tissue specificityi

In ovary, expressed in germline stem cells, germline cyst cells, nurse cells and oocytes during early stages. Also found in the somatic cap cells of the germarium. In testis, expressed in germline stem cells, primary gonial cells and early spermatocytes. No expression detected in the somatic hub cells at the apical tip of the testis (at protein level). Expressed in neurons throughout the adult brain and in the mushroom body subdivision in the peduncle. In the mushroom body, expressed only in gamma and core alpha-beta neurons.5 Publications

Developmental stagei

Expressed maternally. Expression is high in early embryos but gradually decreases as development proceeds (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0250816.
GenevisibleiQ7PLK0. DM.

Interactioni

Subunit structurei

Interacts with aub. Interacts (when methylated on arginine residues) with tud. Forms a complex with smg, twin, aub, nos mRNA and piRNAs that target the nos 3'-untranslated region, in early embryos. Interacts (via the N-terminal region when methylated on arginine residues) with papi; this interaction is RNA-independent and may be required for AGO3 localization to the nuage. Interacts with TER94 and tral.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
papiQ9VQ916EBI-3431981,EBI-6915287
tudP258235EBI-3431981,EBI-498741

Protein-protein interaction databases

IntActiQ7PLK0. 7 interactors.
MINTiMINT-7555877.
STRINGi7227.FBpp0289159.

Structurei

3D structure databases

ProteinModelPortaliQ7PLK0.
SMRiQ7PLK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini286 – 402PAZPROSITE-ProRule annotationAdd BLAST117
Domaini566 – 853PiwiPROSITE-ProRule annotationAdd BLAST288

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 289Interaction with papi1 PublicationAdd BLAST289

Sequence similaritiesi

Belongs to the argonaute family. Piwi subfamily.Curated
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 1 Piwi domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1042. Eukaryota.
ENOG410XNRH. LUCA.
GeneTreeiENSGT00760000119148.
InParanoidiQ7PLK0.
KOiK02156.
OMAiIKRICCS.
OrthoDBiEOG091G020J.
PhylomeDBiQ7PLK0.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform D2 Publications (identifier: Q7PLK0-1) [UniParc]FASTAAdd to basket
Also known as: E1 Publication, F1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGRGNLLSL FNKNAGNMGK SISSKDHEID SGLDFNNSES SGERLLSSHN
60 70 80 90 100
IETDLITTLQ HVNISVGRGR ARLIDTLKTD DHTSNQFITS ESKENITKKT
110 120 130 140 150
KGPESEAIAS ENGLFFPDLI YGSKGSSVNI YCNYLKLTTD ESKGVFNYEV
160 170 180 190 200
RFFPPIDSVH LRIKYLNDHK DKLGGTKTFD GNTLYLPILL PNKMTVFISK
210 220 230 240 250
AEDVELQIRI LYKKKEEMRN CTQLYNILFD RVMKVLNYVK FDRKQFDPSR
260 270 280 290 300
PKIIPLAKLE VWPGYVTAVD EYKGGLMLCC DVSHRILCQK TVLEMLVDLY
310 320 330 340 350
QQNVEHYQES ARKMLVGNIV LTRYNNRTYK INDICFDQNP TCQFEIKTGC
360 370 380 390 400
TSYVEYYKQY HNINIKDVNQ PLIYSIKKSR GIPAERENLQ FCLIPELCYL
410 420 430 440 450
TGLRDEVRSD NKLMREIATF TRVSPNQRQM ALNKFYENVS NTPAAQEILN
460 470 480 490 500
SWGLSLTNNS NKISGRQMDI EQIYFSKISV SAGRSAEFSK HAVTNEMLKV
510 520 530 540 550
VHLSKWIIIH LRNYRQAATS LLDNMKQACE SLGMNISNPT MISLDHDRID
560 570 580 590 600
AYIQALRRNI TMNTQMVVCI CHNRRDDRYA AIKKICCSEI PIPSQVINAK
610 620 630 640 650
TLQNDLKIRS VVQKIVLQMN CKLGGSLWTV KIPFKNVMIC GIDSYHDPSN
660 670 680 690 700
RGNSVAAFVA SINSSYSQWY SKAVVQTKRE EIVNGLSASF EIALKMYRKR
710 720 730 740 750
NGKLPTNIII YRDGIGDGQL YTCLNYEIPQ FEMVCGNRIK ISYIVVQKRI
760 770 780 790 800
NTRIFSGSGI HLENPLPGTV VDQHITKSNM YDFFLVSQLV RQGTVTPTHY
810 820 830 840 850
VVLRDDCNYG PDIIQKLSYK LCFLYYNWAG TVRIPACCMY AHKLAYLIGQ
860
SIQRDVAEAL SEKLFYL
Length:867
Mass (Da):99,129
Last modified:March 3, 2009 - v3
Checksum:iAE613D6C845F78BE
GO
Isoform G2 Publications (identifier: Q7PLK0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     840-867: YAHKLAYLIGQSIQRDVAEALSEKLFYL → VSHNYHLIFFKSTMITQDTINDFTRAL

Show »
Length:866
Mass (Da):99,102
Checksum:i15C5A61602A5702E
GO

Sequence cautioni

The sequence ACJ13248 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence ACK77640 differs from that shown. Contaminating sequence. Sequence of unknown origin in the C-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42G → R in ABO27430 (PubMed:17346786).Curated1
Sequence conflicti168D → G in AFX62827 (Ref. 6) Curated1
Sequence conflicti320V → L in AFX62828 (Ref. 6) Curated1
Sequence conflicti371P → L in AFX62828 (Ref. 6) Curated1
Sequence conflicti543S → L in AFX62828 (Ref. 6) Curated1
Sequence conflicti600K → R in AFX62828 (Ref. 6) Curated1
Sequence conflicti732E → A in AFX62827 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047355840 – 867YAHKL…KLFYL → VSHNYHLIFFKSTMITQDTI NDFTRAL in isoform G. 2 PublicationsAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF211827 mRNA. Translation: ABO27430.1.
EF434174 mRNA. Translation: ABO26294.1.
AE014296 Genomic DNA. Translation: ABI31274.2.
AE014296 Genomic DNA. Translation: ABI31275.2.
AE014296 Genomic DNA. Translation: ACZ94769.1.
AE014296 Genomic DNA. Translation: EAA45981.3.
BT050541 mRNA. Translation: ACJ13248.1. Different initiation.
BT053722 mRNA. Translation: ACK77640.1. Sequence problems.
JX656887 mRNA. Translation: AFX62827.1.
JX656888 mRNA. Translation: AFX62828.1.
EF688531 mRNA. Translation: ABU63676.1.
RefSeqiNP_001036627.2. NM_001043162.3. [Q7PLK0-1]
NP_001036628.2. NM_001043163.3. [Q7PLK0-1]
NP_001036629.2. NM_001043164.3. [Q7PLK0-1]
NP_001163498.1. NM_001170027.2. [Q7PLK0-2]
UniGeneiDm.33791.

Genome annotation databases

EnsemblMetazoaiFBtr0299880; FBpp0289158; FBgn0250816. [Q7PLK0-1]
FBtr0299881; FBpp0289159; FBgn0250816. [Q7PLK0-1]
FBtr0299882; FBpp0289160; FBgn0250816. [Q7PLK0-1]
GeneIDi3355150.
KEGGidme:Dmel_CG40300.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF211827 mRNA. Translation: ABO27430.1.
EF434174 mRNA. Translation: ABO26294.1.
AE014296 Genomic DNA. Translation: ABI31274.2.
AE014296 Genomic DNA. Translation: ABI31275.2.
AE014296 Genomic DNA. Translation: ACZ94769.1.
AE014296 Genomic DNA. Translation: EAA45981.3.
BT050541 mRNA. Translation: ACJ13248.1. Different initiation.
BT053722 mRNA. Translation: ACK77640.1. Sequence problems.
JX656887 mRNA. Translation: AFX62827.1.
JX656888 mRNA. Translation: AFX62828.1.
EF688531 mRNA. Translation: ABU63676.1.
RefSeqiNP_001036627.2. NM_001043162.3. [Q7PLK0-1]
NP_001036628.2. NM_001043163.3. [Q7PLK0-1]
NP_001036629.2. NM_001043164.3. [Q7PLK0-1]
NP_001163498.1. NM_001170027.2. [Q7PLK0-2]
UniGeneiDm.33791.

3D structure databases

ProteinModelPortaliQ7PLK0.
SMRiQ7PLK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7PLK0. 7 interactors.
MINTiMINT-7555877.
STRINGi7227.FBpp0289159.

Proteomic databases

PaxDbiQ7PLK0.
PRIDEiQ7PLK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0299880; FBpp0289158; FBgn0250816. [Q7PLK0-1]
FBtr0299881; FBpp0289159; FBgn0250816. [Q7PLK0-1]
FBtr0299882; FBpp0289160; FBgn0250816. [Q7PLK0-1]
GeneIDi3355150.
KEGGidme:Dmel_CG40300.

Organism-specific databases

CTDi192669.
FlyBaseiFBgn0250816. AGO3.

Phylogenomic databases

eggNOGiKOG1042. Eukaryota.
ENOG410XNRH. LUCA.
GeneTreeiENSGT00760000119148.
InParanoidiQ7PLK0.
KOiK02156.
OMAiIKRICCS.
OrthoDBiEOG091G020J.
PhylomeDBiQ7PLK0.

Miscellaneous databases

GenomeRNAii3355150.
PROiQ7PLK0.

Gene expression databases

BgeeiFBgn0250816.
GenevisibleiQ7PLK0. DM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR003100. PAZ_dom.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTiSM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGO3_DROME
AccessioniPrimary (citable) accession number: Q7PLK0
Secondary accession number(s): A4GND8
, A4GUJ7, A7YFW6, B6IDV1, B7FNN2, K7WKR9, K7XHY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: March 3, 2009
Last modified: November 2, 2016
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

Has been given the gene name AGO3 by FlyBase based on the literature but is more similar to members of the Piwi subfamily.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.