Q7PCJ9 (SAT2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diamine acetyltransferase 2
EC=2.3.1.57

Alternative name(s):
Polyamine N-acetyltransferase 2
Spermidine/spermine N(1)-acetyltransferase 2

Gene names

Name:	SAT2
Synonyms:	SSAT2

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine > spermidine = spermine >> N1acetylspermine = putrescine By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Pathway	Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity. Note: Intracellular organelles By similarity.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Diamine acetyltransferase 2		PRO_0000074600
Regions
	Domain	4 – 166	163	N-acetyltransferase
	Region	27 – 28	2	Substrate binding By similarity
	Region	94 – 96	3	Acetyl-CoA binding By similarity
	Region	102 – 107	6	Acetyl-CoA binding By similarity
	Region	133 – 135	3	Acetyl-CoA binding By similarity
Sites
	Binding site	92	1	Substrate; via carbonyl oxygen By similarity
	Binding site	140	1	Acetyl-CoA By similarity
	Binding site	152	1	Substrate By similarity
Amino acid modifications
	Modified residue	29	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7PCJ9 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 1B1E063A20550568	FASTA	170	19,171
	10 20 30 40 50 60 MASVLIREAK EGDCGNILRM IRELAEYEKL SDQVKISEEA LRADGFGENP FFHCLVAEIL 70 80 90 100 110 120 PAPGEPQGSC MVGYGLYYFI YSTWTGRNIY LEDIYVKPEY RGQGIGSKII KKVAEVALDK 130 140 150 160 170 GCSQFRLAVL DWNKKAVDLY KTLGARDLTE AEGWHSFRFE GEAMRELAGK

References

[1]	"Design and use of two pooled tissue normalized cDNA libraries for EST discovery in swine." Fahrenkrug S.C., Freking B.A., Rohrer G.A., Smith T.P.L., Casas E., Stone R.T., Heaton M.P., Grosse W.M., Bennett G.A., Laegreid W.W., Keele J.W. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-119.
[2]	"Sequence analysis of African swine fever virus infected and non-infected porcine macrophage cDNA libraries." Neilan J.G., Kutish G.F., Lu Z., Zsak A., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 55-170.
[3]	"Genomic identification and biochemical characterization of a second spermidine/spermine N1-acetyltransferase." Chen Y., Vujcic S., Liang P., Diegelman P., Kramer D.L., Porter C.W. Biochem. J. 373:661-667(2003) [PubMed: 12803540] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BF198274 mRNA. No translation available. CB470125 mRNA. No translation available. BK001358 mRNA. Translation: DAA01468.1.
RefSeq	NP_001123434.1. NM_001129962.1.
UniGene	Ssc.10428.
3D structure databases
ProteinModelPortal	Q7PCJ9.
SMR	Q7PCJ9. Positions 2-170.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7PCJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100170117.
KEGG	ssc:100170117.
Organism-specific databases
CTD	112483.
Phylogenomic databases
GeneTree	ENSGT00440000039972.
HOVERGEN	HBG063175.
OrthoDB	EOG47SSG0.
Family and domain databases
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K00657.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT2_PIG

Accession

Primary (citable) accession number: Q7PCJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	December 15, 2003
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program