Reviewed, UniProtKB/Swiss-Prot Q7PCJ8 (SAT2_BOVIN)

Names and origin

Protein names

Recommended name:
    Diamine acetyltransferase 2
    EC=2.3.1.57
Alternative name(s):
    Spermidine/spermine N(1)-acetyltransferase 2
    Polyamine N-acetyltransferase 2

Gene names

Name:	SAT2
Synonyms:	SSAT2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine > spermidine = spermine >> N1acetylspermine = putrescine By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Pathway	Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Note: Intracellular organelles By similarity.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Diamine acetyltransferase 2		PRO_0000074597
Regions
	Domain	4 – 166	163	N-acetyltransferase
	Region	27 – 28	2	Substrate binding By similarity
	Region	94 – 96	3	Acetyl-CoA binding By similarity
	Region	102 – 107	6	Acetyl-CoA binding By similarity
	Region	133 – 135	3	Acetyl-CoA binding By similarity
Sites
	Binding site	92	1	Substrate; via carbonyl oxygen By similarity
	Binding site	140	1	Acetyl-CoA By similarity
	Binding site	152	1	Substrate By similarity
Amino acid modifications
	Modified residue	29	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7PCJ8-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 0DBC86BF794F611D	FASTA	170	19,187
	10 20 30 40 50 60 MAFVMIREAK EGDCGNILRL IRELAEYEKL SDQVKISEEA LRADGFGETP FYHCLVAEIL 70 80 90 100 110 120 SAPGEPQGPC VVGYGLYYFS YSTWKGRNIY LEDIYVKPEY RGQGIGSKII KKVAEVALDK 130 140 150 160 170 GCSQLRLAVL DWNKRAMDLY KALGAQDLTE AEGWHCFRFE GEAMRELAGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.
[2]	"Genomic identification and biochemical characterization of a second spermidine/spermine N1-acetyltransferase." Chen Y., Vujcic S., Liang P., Diegelman P., Kramer D.L., Porter C.W. Biochem. J. 373:661-667(2003) [PubMed: 12803540] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC122800 mRNA. Translation: AAI22801.1. BK001359 mRNA. Translation: DAA01469.1.
IPI	IPI00689794.
RefSeq	NP_976068.1.
UniGene	Bt.11576
3D structure databases
SMR	Q7PCJ8. Positions 2-170.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7PCJ8.
Genome annotation databases
Ensembl	ENSBTAT00000005531; ENSBTAP00000005531; ENSBTAG00000004222; Bos taurus. [Genome view]
GeneID	359722.
KEGG	bta:359722.
Organism-specific databases
CTD	359722.
Phylogenomic databases
HOVERGEN	Q7PCJ8.
OMA	ITAMIRE.
Enzyme and pathway databases
BRENDA	2.3.1.57. 251.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT2_BOVIN

Accession

Primary (citable) accession number: Q7PCJ8
Secondary accession number(s): Q0II54

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)