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Q7P0R1 (SYL_CHRVO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CV_0505
OrganismChromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) [Complete proteome] [HAMAP]
Taxonomic identifier243365 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeChromobacterium

Protein attributes

Sequence length872 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 872872Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152001

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7P0R1 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: C30AC54B2A37BB78

FASTA87296,741
        10         20         30         40         50         60 
MQEQYSPRAV EAAAQQKWQK TAAFKAVEDA SRPKYYALSM FPYPSGKLHM GHVRNYTITD 

        70         80         90        100        110        120 
VLARFKRLQG FNVLQPMGWD AFGLPAENAA MKNGGAPAAW TYANIEYMKT QLDSLGFALD 

       130        140        150        160        170        180 
WERELATCKP DYYRWEQWLF TRLFEKGVIY KKNGVVNWDP VDQTVLANEQ VVDGRGWRSG 

       190        200        210        220        230        240 
ALVEKREIPM YYFRITDYAE QLLADLDKLD GWPEQVKTMQ RNWIGKSYGS DVVFPYDEAS 

       250        260        270        280        290        300 
IGHAGELKVY TTRPDTLMGA TYVAVAAEHP LATQAAAGNA ALQAFIAECK SGSVAEADVA 

       310        320        330        340        350        360 
KMEKKGMDTG LSVIHPLTGE RLPVWVANYV LWGYGEGAVM AVPAHDERDF EFANKYQLPI 

       370        380        390        400        410        420 
KQVIALASGD GEYDAANWQE WYGAKDDTVK TVNSGKYDGL GYQAAFDAII GDLQAKSLGQ 

       430        440        450        460        470        480 
KKTQYRLRDW GISRQRYWGC PIPIIHCPSC GDVPVPEKDL PVTLPENVIP DGAGSPLAKM 

       490        500        510        520        530        540 
PEFYETSCPK CGGAAKRETD TMDTFVESSW YYARYASPKC DTAMVDKQAA DYWLQVDQYV 

       550        560        570        580        590        600 
GGIEHAILHL LYARFFHKLM RDEGLVSSDE PFKSLLTQGM VVCETFYRDL PNGTKDWIAP 

       610        620        630        640        650        660 
QDVILERDAK GKIVAAKHRV DGQPVVVGGI EKMSKSKNNG VDPQEFIEKY GADTARLFMM 

       670        680        690        700        710        720 
FAAPPEQSLE WSDAGVEGAF RFLKRLWKTA REHVEAGVVA PYASGELNAS QKELRFKLHG 

       730        740        750        760        770        780 
TIQKVADDYG RRQQFNTAIA AVMELLNAYD KADTSGDIGR AVAQEVLEAA TLLLSPIVPH 

       790        800        810        820        830        840 
VCDGIWNALK PGTELLAQAW PKVDEAALVK SEIELMVQVC GKLRGSVTVA ADAAKDAIEA 

       850        860        870 
AALAHENVIK FMEGKPAKKI IVVPGRLVNI VV 

« Hide

References

[1]"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability."
Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. expand/collapse author list , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G.
Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016825 Genomic DNA. Translation: AAQ58182.1.
RefSeqNP_900175.1. NC_005085.1.

3D structure databases

ProteinModelPortalQ7P0R1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243365.CV_0505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAQ58182; AAQ58182; CV_0505.
GeneID2551299.
KEGGcvi:CV_0505.
PATRIC21435808. VBIChrVio67196_0505.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCVIO243365:GHUD-515-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CHRVO
AccessionPrimary (citable) accession number: Q7P0R1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: December 15, 2003
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries