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Protein

D-aminoacyl-tRNA deacylase

Gene

dtd

Organism
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.UniRule annotation

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).UniRule annotation
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylaseUniRule annotation (EC:3.1.1.96UniRule annotation)
Short name:
DTDUniRule annotation
Alternative name(s):
Gly-tRNA(Ala) deacylaseUniRule annotation (EC:3.1.1.-UniRule annotation)
Gene namesi
Name:dtdUniRule annotation
Ordered Locus Names:CV_1251
OrganismiChromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Taxonomic identifieri243365 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeChromobacterium
Proteomesi
  • UP000001424 Componenti: Chromosome

Subcellular locationi

Q7NYM3:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001645291 – 150D-aminoacyl-tRNA deacylaseAdd BLAST150

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243365.CV_1251.

Structurei

3D structure databases

ProteinModelPortaliQ7NYM3.
SMRiQ7NYM3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi138 – 139Gly-cisPro motif, important for rejection of L-amino acidsUniRule annotation2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.UniRule annotation

Sequence similaritiesi

Belongs to the DTD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108YYA. Bacteria.
COG1490. LUCA.
HOGENOMiHOG000113982.
KOiK07560.
OMAiDGPVTIW.
OrthoDBiPOG091H02D0.

Family and domain databases

Gene3Di3.50.80.10. 1 hit.
HAMAPiMF_00518. Deacylase_Dtd. 1 hit.
InterProiView protein in InterPro
IPR003732. Daa-tRNA_deacyls_DTD.
IPR023509. DTD-like_dom.
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiView protein in Pfam
PF02580. Tyr_Deacylase. 1 hit.
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7NYM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLVQRVSQ AAVTVDGQTS GEIGAGALLL VGIEESDGPD DIGWLVRKIS
60 70 80 90 100
QLRIFNDEAG VMNRSLIDCG GEALAVSQFT LHASVKKGNR PSYSRAARGE
110 120 130 140 150
ISRPLFDRFV AELSAALGKT VPTGVFGADM RVSLVNDGPV TIWLDSRNPE
Length:150
Mass (Da):16,010
Last modified:December 15, 2003 - v1
Checksum:i9836A732A298138F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016825 Genomic DNA. Translation: AAQ58926.1.
RefSeqiWP_011134805.1. NC_005085.1.

Genome annotation databases

EnsemblBacteriaiAAQ58926; AAQ58926; CV_1251.
GeneIDi24949458.
KEGGicvi:CV_1251.

Similar proteinsi

Entry informationi

Entry nameiDTD_CHRVO
AccessioniPrimary (citable) accession number: Q7NYM3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: December 15, 2003
Last modified: October 25, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families