Q7NX86 (SYQ_CHRVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	CV_1742

Organism

Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) [Complete proteome] [HAMAP]

Taxonomic identifier

243365 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Chromobacterium group › Chromobacterium › Chromobacterium violaceum

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_0000195830
Regions
	Motif	36 – 46	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	270 – 274	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NX86 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 2E23F03ECAB9CEF5	FASTA	560	64,372
	10 20 30 40 50 60 MSTENNAPVV NNFIRSIIDE DLATGRRSSV VTRFPPEPNG FAHIGHAKAI CINFGLAEDY 70 80 90 100 110 120 NGQCNLRMDD TNPEKESDEF VEAFKQDISW LGFKWNGEVR YASDYFDRLY DYAVELIQAG 130 140 150 160 170 180 KAYVDDLSAE EMRQYRGNLT EPGKNSPYRD RTPEENLDLF TRMKNGEFPD GSKTLRLKID 190 200 210 220 230 240 MASGNINLRD PAIYRIRRVH HHRTGDKWCI YPMYDYTHCI SDAIEGITHS LCSLEFEDHR 250 260 270 280 290 300 PLYDWVLDNI SIEHHPQQIE FSRLELLYAL TSKRKLQALV NDGAVTGWDD PRMPTIAGMR 310 320 330 340 350 360 RRGYSPAGIK LFAQRIGVSK SENIIDMAIL EGAVRETLEN DSPRVMAVVN PLKVTLTNYD 370 380 390 400 410 420 AAVTASRSAP FHPHHPEFGE RDVPIAREIW IERDDFAETP PPKWQRLTAG GEVRLRYSYV 430 440 450 460 470 480 IKCDEVVKDA ADEIVELKCS IDHDTLGKNP EGRKVKGVIH WVSAEHAIQA DVRWYERLFT 490 500 510 520 530 540 EQRPDAVRGE DGEYVDFRQF LNPESLKLVP AYVEASVLQA EPESRFQFER LGYFVTDRYE 550 560 HRKGDKAVFN RTVGLKDSWK

References

[1]

"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability." Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016825 Genomic DNA. Translation: AAQ59416.2.
RefSeq	NP_901412.1. NC_005085.1.
3D structure databases
ProteinModelPortal	Q7NX86.
SMR	Q7NX86. Positions 11-558.
ModBase	Search...
Protein-protein interaction databases
STRING	243365.CV_1742.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAQ59416; AAQ59416; CV_1742.
GeneID	2551030.
KEGG	cvi:CV_1742.
PATRIC	21438290. VBIChrVio67196_1706.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	CVIO243365:GHUD-1741-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_CHRVO

Accession

Primary (citable) accession number: Q7NX86

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program