ID   Q7NWG4_CHRVO            Unreviewed;       459 AA.
AC   Q7NWG4;
DT   15-DEC-2003, integrated into UniProtKB/TrEMBL.
DT   15-DEC-2003, sequence version 1.
DT   27-MAR-2024, entry version 127.
DE   RecName: Full=Putative 8-amino-7-oxononanoate synthase {ECO:0000256|ARBA:ARBA00021531};
GN   OrderedLocusNames=CV_2025 {ECO:0000313|EMBL:AAQ59697.1};
OS   Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC
OS   12614 / NCIMB 9131 / NCTC 9757).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
OC   Chromobacteriaceae; Chromobacterium.
OX   NCBI_TaxID=243365 {ECO:0000313|EMBL:AAQ59697.1, ECO:0000313|Proteomes:UP000001424};
RN   [1] {ECO:0000313|EMBL:AAQ59697.1, ECO:0000313|Proteomes:UP000001424}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 /
RC   NCTC 9757 {ECO:0000313|Proteomes:UP000001424};
RX   PubMed=14500782; DOI=10.1073/pnas.1832124100;
RA   Vasconcelos A.T.R., de Almeida D.F., Almeida F.C., de Almeida L.G.P.,
RA   de Almeida R., Goncalves J.A.A., Andrade E.M., Antonio R.V., Araripe J.,
RA   de Araujo M.F.F., Filho S.A., Azevedo V., Batista A.J., Bataus L.A.M.,
RA   Batista J.S., Belo A., vander Berg C., Blamey J., Bogo M., Bonato S.,
RA   Bordignon J., Brito C.A., Brocchi M., Burity H.A., Camargo A.A.,
RA   Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B.,
RA   Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Pasa T.B.C., Duran N.,
RA   Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S.,
RA   Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A.,
RA   Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B.,
RA   Grattapaglia D., Grisard E.C., Guimaraes C.T., Hanna E.S., Hungria M.,
RA   Jardim S.N., Laurino J., Leoi L.C.T., Fassarella L., Lima A.,
RA   Loureiro M.F., Lyra M.C.P., Macedo M., Madeira H.M.F., Manfio G.P.,
RA   Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B.,
RA   Meissner R.D.V., Menck C.F.M., Moreira M.A.M., Nascimento F.F.,
RA   Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A.,
RA   Pedrosa F.O., Pena S.J.D., Perreira J.O., Perreira M., Pinto L.S.R.C.,
RA   Pinto L.S., Porto J.I.R., Potrich D.P., Neto C.E.R., Reis A.M.M.,
RA   Rigo L.U., Rondinelli E., dos Santos E.B.P., Santos F.R., Schneider M.P.C.,
RA   Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R.,
RA   Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M.,
RA   Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R.,
RA   Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G.;
RT   "The complete genome sequence of Chromobacterium violaceum reveals
RT   remarkable and exploitable bacterial adaptability.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003).
RN   [2] {ECO:0007829|PDB:4A6R, ECO:0007829|PDB:4A6T}
RP   X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS), AND PYRIDOXAL PHOSPHATE AT LYS-288.
RX   PubMed=22268978; DOI=10.1111/j.1742-4658.2012.08468.x;
RA   Humble M.S., Cassimjee K.E., Hakansson M., Kimbung Y.R., Walse B.,
RA   Abedi V., Federsel H.J., Berglund P., Logan D.T.;
RT   "Crystal structures of the Chromobacterium violaceumomega-transaminase
RT   reveal major structural rearrangements upon binding of coenzyme PLP.";
RL   FEBS J. 279:779-792(2012).
RN   [3] {ECO:0007829|PDB:4AH3, ECO:0007829|PDB:4BA4}
RP   X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS), AND PYRIDOXAL PHOSPHATE AT LYS-288.
RX   PubMed=23519665; DOI=10.1107/S0907444912051670;
RA   Sayer C., Isupov M.N., Westlake A., Littlechild J.A.;
RT   "Structural studies of Pseudomonas and Chromobacterium omega-
RT   aminotransferases provide insights into their differing substrate
RT   specificity.";
RL   Acta Crystallogr. D 69:564-576(2013).
RN   [4] {ECO:0007829|PDB:6S4G}
RP   X-RAY CRYSTALLOGRAPHY (1.67 ANGSTROMS), AND PYRIDOXAL PHOSPHATE AT GLY-120;
RP   SER-121 AND THR-321.
RX   PubMed=31740704; DOI=10.1038/s41598-019-53177-3;
RA   Ruggieri F., Campillo-Brocal J.C., Chen S., Humble M.S., Walse B.,
RA   Logan D.T., Berglund P.;
RT   "Insight into the dimer dissociation process of the Chromobacterium
RT   violaceum (S)-selective amine transaminase.";
RL   Sci. Rep. 9:16946-16946(2019).
RN   [5] {ECO:0007829|PDB:7Q9X, ECO:0007829|PDB:7Q9Z}
RP   X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH PYRIDOXAL
RP   5'-PHOSPHATE.
RA   Isupov M.N., Mitchell D., Sayer C., Littlechild J.A.;
RT   "Aminotransferase from Chromobacterium violaceum in complex with PLP-
RT   pyruvate adduct.";
RL   Submitted (NOV-2021) to the PDB data bank.
CC   -!- INTERACTION:
CC       Q7NWG4; Q7NWG4: CV_2025; NbExp=3; IntAct=EBI-7663449, EBI-7663449;
CC   -!- SIMILARITY: Belongs to the class-III pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000256|ARBA:ARBA00008954,
CC       ECO:0000256|RuleBase:RU003560}.
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DR   EMBL; AE016825; AAQ59697.1; -; Genomic_DNA.
DR   RefSeq; WP_011135573.1; NC_005085.1.
DR   PDB; 4A6R; X-ray; 1.35 A; A/B=1-459.
DR   PDB; 4A6T; X-ray; 1.80 A; A/B/C/D=1-459.
DR   PDB; 4A6U; X-ray; 1.69 A; A/B=1-459.
DR   PDB; 4A72; X-ray; 2.40 A; A/B/C/D=1-459.
DR   PDB; 4AH3; X-ray; 1.57 A; A/B/C/D=1-459.
DR   PDB; 4BA4; X-ray; 1.73 A; A/B=1-459.
DR   PDB; 4BA5; X-ray; 1.76 A; A/B=1-459.
DR   PDB; 6S4G; X-ray; 1.67 A; A/B/C/D=1-459.
DR   PDB; 7Q9X; X-ray; 1.60 A; AAA/BBB/CCC/DDD=1-459.
DR   PDB; 7Q9Z; X-ray; 1.95 A; AAA/BBB=1-459.
DR   PDBsum; 4A6R; -.
DR   PDBsum; 4A6T; -.
DR   PDBsum; 4A6U; -.
DR   PDBsum; 4A72; -.
DR   PDBsum; 4AH3; -.
DR   PDBsum; 4BA4; -.
DR   PDBsum; 4BA5; -.
DR   PDBsum; 6S4G; -.
DR   AlphaFoldDB; Q7NWG4; -.
DR   SMR; Q7NWG4; -.
DR   MINT; Q7NWG4; -.
DR   STRING; 243365.CV_2025; -.
DR   GeneID; 66367689; -.
DR   KEGG; cvi:CV_2025; -.
DR   eggNOG; COG0161; Bacteria.
DR   HOGENOM; CLU_016922_4_1_4; -.
DR   OrthoDB; 3398487at2; -.
DR   Proteomes; UP000001424; Chromosome.
DR   GO; GO:0004015; F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   CDD; cd00610; OAT_like; 1.
DR   Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR   Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR   InterPro; IPR005814; Aminotrans_3.
DR   InterPro; IPR049704; Aminotrans_3_PPA_site.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   PANTHER; PTHR43094; AMINOTRANSFERASE; 1.
DR   PANTHER; PTHR43094:SF1; AMINOTRANSFERASE CLASS-III; 1.
DR   Pfam; PF00202; Aminotran_3; 1.
DR   PIRSF; PIRSF000521; Transaminase_4ab_Lys_Orn; 2.
DR   SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR   PROSITE; PS00600; AA_TRANSFER_CLASS_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4A6R, ECO:0007829|PDB:4A6T};
KW   Aminotransferase {ECO:0000313|EMBL:AAQ59697.1};
KW   Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898,
KW   ECO:0000256|RuleBase:RU003560};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001424};
KW   Transferase {ECO:0000313|EMBL:AAQ59697.1}.
FT   BINDING         120
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0007829|PDB:4A6T, ECO:0007829|PDB:4A72"
FT   BINDING         121
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0007829|PDB:4A6T, ECO:0007829|PDB:4A72"
FT   BINDING         288
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /ligand_note="covalent"
FT                   /evidence="ECO:0007829|PDB:4A6T, ECO:0007829|PDB:4A72"
FT   BINDING         321
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0007829|PDB:4A6T, ECO:0007829|PDB:4A72"
FT   MOD_RES         120
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0007829|PDB:6S4G"
FT   MOD_RES         121
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0007829|PDB:6S4G"
FT   MOD_RES         321
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0007829|PDB:6S4G"
SQ   SEQUENCE   459 AA;  51213 MW;  5CD2CC1AF273D260 CRC64;
     MQKQRTTSQW RELDAAHHLH PFTDTASLNQ AGARVMTRGE GVYLWDSEGN KIIDGMAGLW
     CVNVGYGRKD FAEAARRQME ELPFYNTFFK TTHPAVVELS SLLAEVTPAG FDRVFYTNSG
     SESVDTMIRM VRRYWDVQGK PEKKTLIGRW NGYHGSTIGG ASLGGMKYMH EQGDLPIPGM
     AHIEQPWWYK HGKDMTPDEF GVVAARWLEE KILEIGADKV AAFVGEPIQG AGGVIVPPAT
     YWPEIERICR KYDVLLVADE VICGFGRTGE WFGHQHFGFQ PDLFTAAKGL SSGYLPIGAV
     FVGKRVAEGL IAGGDFNHGF TYSGHPVCAA VAHANVAALR DEGIVQRVKD DIGPYMQKRW
     RETFSRFEHV DDVRGVGMVQ AFTLVKNKAK RELFPDFGEI GTLCRDIFFR NNLIMRACGD
     HIVSAPPLVM TRAEVDEMLA VAERCLEEFE QTLKARGLA
//