Q7NVN5 (CYSN_CHRVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 1
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase large subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysN
Ordered Locus Names:	CV_2307

Organism

Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) [Complete proteome] [HAMAP]

Taxonomic identifier

243365 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Chromobacterium group › Chromobacterium › Chromobacterium violaceum

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be the GTPase, regulating ATP sulfurylase activity By similarity. HAMAP-Rule MF_00062
Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP-Rule MF_00062
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP-Rule MF_00062
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. CysN/NodQ subfamily.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Sulfate adenylyltransferase subunit 1 HAMAP-Rule MF_00062		PRO_0000091521
Regions
	Nucleotide binding	31 – 38	8	GTP By similarity
	Nucleotide binding	110 – 114	5	GTP By similarity
	Nucleotide binding	165 – 168	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NVN5 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 2256306774D4BE87	FASTA	477	52,841
	10 20 30 40 50 60 MSHQSELIAG DILEYLSQHE NKDMLRFITC GSVDDGKSTL IGRLLHDSKL IFEDQLAAIE 70 80 90 100 110 120 RDSKKYNTTD QDIDLALLVD GLQAEREQGI TIDVAYRYFS TDKRKFIIAD CPGHEQYTRN 130 140 150 160 170 180 MATGASTSNL AIILIDARRG VQTQTRRHSY IVSLLGIRHV IVAVNKMDLV DYSEDVYNRI 190 200 210 220 230 240 RDEYLTFAEH LDIPDIHFVP ISALRGDNVV SRTEAAPWYQ GATLMHLLET VQISHDAPLS 250 260 270 280 290 300 AFRLPVQYVN RPNLDFRGFC GTIASGAVHP GDAVVALPSG KESRVKEIVT FDGSLSRAGA 310 320 330 340 350 360 GQAVTLTLED EIDISRGDML VRADEARPHV SRSFDAHLVA MGEAPLRPGK EYGFKLAGKY 370 380 390 400 410 420 VTGRIEAILH RTDVNTLQDS PADALALNEI GLCRISLNSP AAFDAYRDCR GSGSLILIDR 430 440 450 460 470 LSNGTVAAGM IVAQSEEASP AALSPWALFE QELDQLLQRY FPQMTRAELA RRLRDEL

References

[1]

"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability." Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016825 Genomic DNA. Translation: AAQ59979.1.
RefSeq	NP_901977.1. NC_005085.1.
3D structure databases
ProteinModelPortal	Q7NVN5.
SMR	Q7NVN5. Positions 16-433.
ModBase	Search...
Protein-protein interaction databases
STRING	243365.CV_2307.
Proteomic databases
PRIDE	Q7NVN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAQ59979; AAQ59979; CV_2307.
GeneID	2549880.
KEGG	cvi:CV_2307.
PATRIC	21439382. VBIChrVio67196_2247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2895.
HOGENOM	HOG000229289.
KO	K00956.
OMA	MHESPLE.
ProtClustDB	CLSK2485475.
Enzyme and pathway databases
BioCyc	CVIO243365:GHUD-2306-MONOMER.
UniPathway	UPA00140; UER00204.
Family and domain databases
HAMAP	MF_00062. Sulf_adenylyltr_sub1.
InterPro	IPR000795. EF_GTP-bd_dom. IPR011779. SO4_adenylTrfase_lsu. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR02034. CysN. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSN_CHRVO

Accession

Primary (citable) accession number: Q7NVN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program