Q7NUB8 (LEUD2_CHRVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase small subunit 2
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase 2
Short name=IPMI 2
Isopropylmalate isomerase 2

Gene names

Name:	leuD2
Ordered Locus Names:	CV_2782

Organism

Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) [Complete proteome] [HAMAP]

Taxonomic identifier

243365 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Chromobacterium group › Chromobacterium › Chromobacterium violaceum

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01031
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01031
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01031
Subunit structure	Heterodimer of LeuC and LeuD By similarity.
Sequence similarities	Belongs to the LeuD family. LeuD type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	3-isopropylmalate dehydratase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	3-isopropylmalate dehydratase small subunit 2 HAMAP-Rule MF_01031		PRO_0000141811

Sequences

	Sequence		Length	Mass (Da)
	Q7NUB8 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: FD5CB8726E1980C2	FASTA	212	23,768
	10 20 30 40 50 60 MKAFTTLQGL VCPLDRANVD TDAIIPKQFL KSIKRSGFGP NLFDEWRYLD HGEPGMDNAK 70 80 90 100 110 120 RPLNPDFVLN QPRYQGAQVL LARENFGCGS SREHAPWALD DQGFRVVIAP SFADIFFNNC 130 140 150 160 170 180 YKNGLLPIVL AADIVDQLFQ ECEAAPGYSL KVDLAAQTVF TPSGQAFLFD ITEHRKHCLL 190 200 210 GGLDEIGLTL QHADEIKAFE ARRRVEQPWL FA

References

[1]

"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability." Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016825 Genomic DNA. Translation: AAQ60450.1.
RefSeq	NP_902452.1. NC_005085.1.
3D structure databases
ProteinModelPortal	Q7NUB8.
ModBase	Search...
Protein-protein interaction databases
STRING	243365.CV_2782.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAQ60450; AAQ60450; CV_2782.
GeneID	2548220.
KEGG	cvi:CV_2782.
PATRIC	21440306. VBIChrVio67196_2705.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0066.
HOGENOM	HOG000222939.
KO	K01704.
OMA	YQDNSKR.
ProtClustDB	PRK01641.
Enzyme and pathway databases
BioCyc	CVIO243365:GHUD-2781-MONOMER.
UniPathway	UPA00048; UER00071.
Family and domain databases
Gene3D	3.20.19.10. 1 hit.
HAMAP	MF_01031. LeuD_type1.
InterPro	IPR004431. 3-IsopropMal_deHydase_ssu. IPR015937. Acoase/IPM_deHydtase. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
PANTHER	PTHR11670. PTHR11670. 1 hit. PTHR11670:SF2. PTHR11670:SF2. 1 hit.
Pfam	PF00694. Aconitase_C. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00171. leuD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEUD2_CHRVO

Accession

Primary (citable) accession number: Q7NUB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program