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Protein

Succinylglutamate desuccinylase

Gene

astE

Organism
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transforms N(2)-succinylglutamate into succinate and glutamate.UniRule annotation

Catalytic activityi

N-succinyl-L-glutamate + H2O = succinate + L-glutamate.UniRule annotation

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 5 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. N-succinylarginine dihydrolase (astB)
  3. no protein annotated in this organism
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Zinc1
Metal bindingi59Zinc1
Metal bindingi149Zinc1
Active sitei214UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00185; UER00283.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinylglutamate desuccinylaseUniRule annotation (EC:3.5.1.96UniRule annotation)
Gene namesi
Name:astEUniRule annotation
Ordered Locus Names:CV_2877
OrganismiChromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Taxonomic identifieri243365 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeChromobacterium
Proteomesi
  • UP000001424 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746381 – 333Succinylglutamate desuccinylaseAdd BLAST333

Interactioni

Protein-protein interaction databases

STRINGi243365.CV_2877.

Structurei

Secondary structure

1333
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6
Beta strandi20 – 23Combined sources4
Turni24 – 26Combined sources3
Beta strandi27 – 33Combined sources7
Beta strandi36 – 40Combined sources5
Beta strandi48 – 53Combined sources6
Helixi61 – 74Combined sources14
Beta strandi82 – 88Combined sources7
Helixi91 – 96Combined sources6
Beta strandi101 – 103Combined sources3
Helixi105 – 107Combined sources3
Turni109 – 111Combined sources3
Helixi112 – 115Combined sources4
Helixi120 – 137Combined sources18
Beta strandi143 – 153Combined sources11
Beta strandi155 – 163Combined sources9
Helixi174 – 181Combined sources8
Turni182 – 184Combined sources3
Beta strandi187 – 190Combined sources4
Helixi198 – 205Combined sources8
Beta strandi209 – 217Combined sources9
Helixi226 – 229Combined sources4
Helixi230 – 241Combined sources12
Turni252 – 254Combined sources3
Beta strandi257 – 265Combined sources9
Beta strandi307 – 310Combined sources4
Beta strandi317 – 328Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YW4X-ray2.00A/B1-333[»]
ProteinModelPortaliQ7NU26.
SMRiQ7NU26.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7NU26.

Family & Domainsi

Sequence similaritiesi

Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107UI5. Bacteria.
COG2988. LUCA.
HOGENOMiHOG000280300.
KOiK05526.
OMAiAHAFTVE.
OrthoDBiPOG091H04VJ.

Family and domain databases

CDDicd03855. M14_ASTE. 1 hit.
HAMAPiMF_00767. Arg_catab_AstE. 1 hit.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
TIGRFAMsiTIGR03242. arg_catab_astE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7NU26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHSPSFLQH ALSSSDTRAE WPLPGGLAAR WLAPGCVELN GDARGADSVL
60 70 80 90 100
LSCGVHGNET APIEVVDGML TDIAAGQLAL NCRLLVMFAN LDAIRQGVRY
110 120 130 140 150
GNYDMNRLFN GAHARHPELP ESVRAAELET LAAEFFAGAR ARKLHYDLHT
160 170 180 190 200
AIRGSVFEKF AIYPFLHDGR THKREQLAWL QRCGIEAVLL HTQPANTFSY
210 220 230 240 250
FTSQYCEADA FTLELGKARP FGQNDLSRFS GIDGALRGLL SNPQANVPDL
260 270 280 290 300
DEDKLPLFRA KYDLVKHSEA FKLNLADSVE NFTLLPDGML IAEDGAVRYQ
310 320 330
ATGGEERILF PNPAVKPGLR AGIVVEPARL PSR
Length:333
Mass (Da):36,520
Last modified:December 15, 2003 - v1
Checksum:iEE3CD3D2F4C1AA9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016825 Genomic DNA. Translation: AAQ60545.1.
RefSeqiWP_011136424.1. NC_005085.1.

Genome annotation databases

EnsemblBacteriaiAAQ60545; AAQ60545; CV_2877.
GeneIDi24947233.
KEGGicvi:CV_2877.
PATRICi21440496. VBIChrVio67196_2800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016825 Genomic DNA. Translation: AAQ60545.1.
RefSeqiWP_011136424.1. NC_005085.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YW4X-ray2.00A/B1-333[»]
ProteinModelPortaliQ7NU26.
SMRiQ7NU26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243365.CV_2877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAQ60545; AAQ60545; CV_2877.
GeneIDi24947233.
KEGGicvi:CV_2877.
PATRICi21440496. VBIChrVio67196_2800.

Phylogenomic databases

eggNOGiENOG4107UI5. Bacteria.
COG2988. LUCA.
HOGENOMiHOG000280300.
KOiK05526.
OMAiAHAFTVE.
OrthoDBiPOG091H04VJ.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00283.

Miscellaneous databases

EvolutionaryTraceiQ7NU26.

Family and domain databases

CDDicd03855. M14_ASTE. 1 hit.
HAMAPiMF_00767. Arg_catab_AstE. 1 hit.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
TIGRFAMsiTIGR03242. arg_catab_astE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASTE_CHRVO
AccessioniPrimary (citable) accession number: Q7NU26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: December 15, 2003
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.