Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Succinylglutamate desuccinylase

Gene

astE

Organism
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transforms N(2)-succinylglutamate into succinate and glutamate.UniRule annotation

Catalytic activityi

N-succinyl-L-glutamate + H2O = succinate + L-glutamate.UniRule annotation

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 5 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. N-succinylarginine dihydrolase (astB)
  3. no protein annotated in this organism
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Zinc
Metal bindingi59 – 591Zinc
Metal bindingi149 – 1491Zinc
Active sitei214 – 2141UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Arginine metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCVIO243365:GHUD-2936-MONOMER.
UniPathwayiUPA00185; UER00283.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinylglutamate desuccinylaseUniRule annotation (EC:3.5.1.96UniRule annotation)
Gene namesi
Name:astEUniRule annotation
Ordered Locus Names:CV_2877
OrganismiChromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Taxonomic identifieri243365 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeChromobacterium
Proteomesi
  • UP000001424 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Succinylglutamate desuccinylasePRO_0000174638Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243365.CV_2877.

Structurei

Secondary structure

1
333
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 126Combined sources
Beta strandi20 – 234Combined sources
Turni24 – 263Combined sources
Beta strandi27 – 337Combined sources
Beta strandi36 – 405Combined sources
Beta strandi48 – 536Combined sources
Helixi61 – 7414Combined sources
Beta strandi82 – 887Combined sources
Helixi91 – 966Combined sources
Beta strandi101 – 1033Combined sources
Helixi105 – 1073Combined sources
Turni109 – 1113Combined sources
Helixi112 – 1154Combined sources
Helixi120 – 13718Combined sources
Beta strandi143 – 15311Combined sources
Beta strandi155 – 1639Combined sources
Helixi174 – 1818Combined sources
Turni182 – 1843Combined sources
Beta strandi187 – 1904Combined sources
Helixi198 – 2058Combined sources
Beta strandi209 – 2179Combined sources
Helixi226 – 2294Combined sources
Helixi230 – 24112Combined sources
Turni252 – 2543Combined sources
Beta strandi257 – 2659Combined sources
Beta strandi307 – 3104Combined sources
Beta strandi317 – 32812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YW4X-ray2.00A/B1-333[»]
ProteinModelPortaliQ7NU26.
SMRiQ7NU26. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7NU26.

Family & Domainsi

Sequence similaritiesi

Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107UI5. Bacteria.
COG2988. LUCA.
HOGENOMiHOG000280300.
KOiK05526.
OMAiAHAFTVE.
OrthoDBiPOG091H04VJ.

Family and domain databases

HAMAPiMF_00767. Arg_catab_AstE. 1 hit.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
TIGRFAMsiTIGR03242. arg_catab_astE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7NU26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHSPSFLQH ALSSSDTRAE WPLPGGLAAR WLAPGCVELN GDARGADSVL
60 70 80 90 100
LSCGVHGNET APIEVVDGML TDIAAGQLAL NCRLLVMFAN LDAIRQGVRY
110 120 130 140 150
GNYDMNRLFN GAHARHPELP ESVRAAELET LAAEFFAGAR ARKLHYDLHT
160 170 180 190 200
AIRGSVFEKF AIYPFLHDGR THKREQLAWL QRCGIEAVLL HTQPANTFSY
210 220 230 240 250
FTSQYCEADA FTLELGKARP FGQNDLSRFS GIDGALRGLL SNPQANVPDL
260 270 280 290 300
DEDKLPLFRA KYDLVKHSEA FKLNLADSVE NFTLLPDGML IAEDGAVRYQ
310 320 330
ATGGEERILF PNPAVKPGLR AGIVVEPARL PSR
Length:333
Mass (Da):36,520
Last modified:December 15, 2003 - v1
Checksum:iEE3CD3D2F4C1AA9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016825 Genomic DNA. Translation: AAQ60545.1.
RefSeqiWP_011136424.1. NC_005085.1.

Genome annotation databases

EnsemblBacteriaiAAQ60545; AAQ60545; CV_2877.
GeneIDi24947233.
KEGGicvi:CV_2877.
PATRICi21440496. VBIChrVio67196_2800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016825 Genomic DNA. Translation: AAQ60545.1.
RefSeqiWP_011136424.1. NC_005085.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YW4X-ray2.00A/B1-333[»]
ProteinModelPortaliQ7NU26.
SMRiQ7NU26. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243365.CV_2877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAQ60545; AAQ60545; CV_2877.
GeneIDi24947233.
KEGGicvi:CV_2877.
PATRICi21440496. VBIChrVio67196_2800.

Phylogenomic databases

eggNOGiENOG4107UI5. Bacteria.
COG2988. LUCA.
HOGENOMiHOG000280300.
KOiK05526.
OMAiAHAFTVE.
OrthoDBiPOG091H04VJ.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00283.
BioCyciCVIO243365:GHUD-2936-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ7NU26.

Family and domain databases

HAMAPiMF_00767. Arg_catab_AstE. 1 hit.
InterProiIPR007036. Aste_AspA.
IPR016681. SuccinylGlu_desuccinylase.
[Graphical view]
PfamiPF04952. AstE_AspA. 1 hit.
[Graphical view]
PIRSFiPIRSF017020. AstE. 1 hit.
TIGRFAMsiTIGR03242. arg_catab_astE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASTE_CHRVO
AccessioniPrimary (citable) accession number: Q7NU26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: December 15, 2003
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.