Reviewed, UniProtKB/Swiss-Prot Q7NTY1 (GUAC_CHRVO)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	CV_2922

Organism

Chromobacterium violaceum [Complete proteome] [HAMAP]

Taxonomic identifier

536 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Chromobacterium

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	GMP reductase HAMAP MF_01511		PRO_0000294271
Regions
	Nucleotide binding	202 – 225	24	NADP Potential
Sites
	Active site	175	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NTY1-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: B010B6BA09FC9A8A	FASTA	316	34,582
	10 20 30 40 50 60 MIKTELNYGD VYLVPKKTVV DSRKECDTSV QFGPRRFAMP VYPSNMKSVV SAETCELFAR 70 80 90 100 110 120 EGWFYTLHRF NVDAVAFTRY MQEQGLFASI SVGVNDDTYE QLDALKAAGL SPEYMTLDIA 130 140 150 160 170 180 NAWCVKAERM IKHIKQHFPN TFLIGGNVAT AEAARDLEAW GCDAIKAGIA GGRVCITKNK 190 200 210 220 230 240 TGFHRPMVST VRDCVAAVTI PVIADGGIVE HGDIAKALVC GATMVMAGSL FAGYDESAGD 250 260 270 280 290 300 IVEIAGKHYK EYFGSASQFN KGAYVNVEGK KILVEYKGSM GKLLRELQED LQSSVSYAGG 310 TTLAALREVE MIQVYR

References

[1]

"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability." Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed: 14500782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12472 / DSM 30191 / IFO 12614 / JCM 1249 / NCIB 9131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016825 Genomic DNA. Translation: AAQ60590.1.
RefSeq	NP_902592.1.
3D structure databases
HSSP	HSSP built from PDB template 1YPF based on UniProtKB Q81JJ9.
SMR	Q7NTY1. Positions 5-315.
ModBase	Search...
Genome annotation databases
GeneID	2547468.
GenomeReviews	Gene locus CV_2922 in contig AE016825_GR.
KEGG	cvi:CV_2922.
NMPDR	fig|243365.1.peg.2922.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298985.
OMA	FAGHEES.
Enzyme and pathway databases
BioCyc	CVIO243365:CV_2922-MONOMER.
BRENDA	1.7.1.7. 415.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_CHRVO

Accession

Primary (citable) accession number: Q7NTY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	December 15, 2003
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)