Reviewed, UniProtKB/Swiss-Prot Q7NS23 (METE_CHRVO)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	CV_3604

Organism

Chromobacterium violaceum [Complete proteome] [HAMAP]

Taxonomic identifier

536 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Chromobacterium

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098625
Sites
	Metal binding	643	1	Zinc By similarity
	Metal binding	645	1	Zinc By similarity
	Metal binding	728	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NS23-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 3B4E621C52B06B6C	FASTA	760	83,581
	10 20 30 40 50 60 MSHATHLLGF PRIGAKRELK TLLERYWKQE LDEAALSQGA KELRQKHWLL QKGAGVELSP 70 80 90 100 110 120 VGDFSLYDHV LDAQLLVGAA PARFGFDAAA LTTGQYFELA RGNAVQPAME MTKWFDTNYH 130 140 150 160 170 180 YLVPEWHADT AFSAQPERLL SQLREARALG VAAKPVLLGP LSLLWLGKAK GRPFDRLALL 190 200 210 220 230 240 PGLVAAYREL LSSLRAAGAE WAQIDEPILA LDLEPSWLDA FAPAYAQLSR HAPKLLLATY 250 260 270 280 290 300 FGDVSEHAAR LKSLPVAGLH LDLVRAPEQI SAFLPDYPAD KVLSAGIVDG RNIWRADLSA 310 320 330 340 350 360 LLDRLAPLAQ QLGDRLWLAP SCSLLHSPFD AAAETGLDPE LKNWLAFAVQ KLNELKTLKR 370 380 390 400 410 420 GLEHGRSAIA GELSGSDFAR EQRRASPRIH DPAVARRLAA LPEGTDRRAS PYPIRAERQQ 430 440 450 460 470 480 AWLKLPPLPT TTIGSFPQTP AIRASRAAFK KGELSAAGYR QAMEKEIELA IRRQEALGLD 490 500 510 520 530 540 VLVHGEAERN DMVEYFGEQL AGFAFTAGGW VQSYGSRCVK PPIIFGDVAR PKPMTVDWAR 550 560 570 580 590 600 YAQSLTAKPV KGMLTGPVTI LQWSFVRDDL PRREVCRQIA LALNDEVLDL EAAGIRVIQI 610 620 630 640 650 660 DEPAIREGLP LKRAGRDVYL AWAGEAFRLS SRGVDDATQI HTHMCYSEFG DILPAIAALD 670 680 690 700 710 720 ADVITIETSR SDMALLADFG RFRYPNAIGP GVYDIHSPRV PSAAEIRALL DKALRVIPAE 730 740 750 760 RLWVNPDCGL KTRGWPEVEA ALAAMVAVGR ELREKLAQAA

References

[1]

"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability." Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed: 14500782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12472 / DSM 30191 / IFO 12614 / JCM 1249 / NCIB 9131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016825 Genomic DNA. Translation: AAQ61266.1.
RefSeq	NP_903274.1.
3D structure databases
SMR	Q7NS23. Positions 4-755.
ModBase	Search...
Genome annotation databases
GeneID	2548455.
GenomeReviews	Gene locus CV_3604 in contig AE016825_GR.
KEGG	cvi:CV_3604.
NMPDR	fig|243365.1.peg.3604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	SFVRNDI.
PhylomeDB	Q7NS23.
Enzyme and pathway databases
BioCyc	CVIO243365:CV_3604-MONOMER.
BRENDA	2.1.1.14. 415.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CHRVO

Accession

Primary (citable) accession number: Q7NS23

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)