Reviewed, UniProtKB/Swiss-Prot Q7NRJ5 (TRMB_CHRVO)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	CV_3786

Organism

Chromobacterium violaceum [Complete proteome] [HAMAP]

Taxonomic identifier

536 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Chromobacterium

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000171318
Regions
	Region	140 – 145	6	Interaction with RNA Potential
	Region	205 – 208	4	Substrate binding Potential
Sites
	Binding site	59	1	S-adenosyl-L-methionine By similarity
	Binding site	84	1	S-adenosyl-L-methionine By similarity
	Binding site	111	1	S-adenosyl-L-methionine By similarity
	Binding site	134	1	S-adenosyl-L-methionine By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	170	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7NRJ5-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: E7D56DCE21789DD9	FASTA	226	25,380
	10 20 30 40 50 60 MENPAFKRAI RSFVLRQGHL SAGQQRAMDE GMPKWGIEYR PETMDLEQVF GRAAPKILEI 70 80 90 100 110 120 GFGMGGATAE IAAANPDNDY LGIEVHGPGV GNLCKLIAEK ELTNLRLMRH DAVEVLDNML 130 140 150 160 170 180 ADGSLDGVHI FFPDPWHKKR HNKRRLIQAP LVEKLAKKLK PGGYFHAATD WEDYAIQILE 190 200 210 220 VLNGNADLEN TADGYAPRPD YRPLTKFEAR GIKLGHGVWD VIFRRK

References

[1]

"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability." Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed: 14500782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12472 / DSM 30191 / IFO 12614 / JCM 1249 / NCIB 9131.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016825 Genomic DNA. Translation: AAQ61448.1.
RefSeq	NP_903456.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2547830.
GenomeReviews	Gene locus CV_3786 in contig AE016825_GR.
KEGG	cvi:CV_3786.
NMPDR	fig|243365.1.peg.3786.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NRJ5.
OMA	Q7NRJ5. TKFENRG.
Enzyme and pathway databases
BioCyc	CVIO243365:CV_3786-MON.
BRENDA	2.1.1.33. 415.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_CHRVO

Accession

Primary (citable) accession number: Q7NRJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)