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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Metal bindingi69MagnesiumUniRule annotation1
Metal bindingi141MagnesiumUniRule annotation1
Metal bindingi144MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:CV_4225
OrganismiChromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Taxonomic identifieri243365 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeChromobacterium
Proteomesi
  • UP000001424 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001830911 – 176Crossover junction endodeoxyribonuclease RuvCAdd BLAST176

Interactioni

Protein-protein interaction databases

STRINGi243365.CV_4225.

Structurei

3D structure databases

ProteinModelPortaliQ7NQB5.
SMRiQ7NQB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012181.
KOiK01159.
OMAiGLTRCGI.
OrthoDBiPOG091H0202.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiView protein in InterPro
IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
PANTHERiPTHR30194:SF4. PTHR30194:SF4. 1 hit.
PfamiView protein in Pfam
PF02075. RuvC. 1 hit.
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiView protein in PROSITE
PS01321. RUVC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7NQB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRILGIDP GSLITGFGVI DVVGNQRHYV ASGAIRTKSG SPLAERVKVL
60 70 80 90 100
VDGIHQVIDT YRPTEAALER VFVNVNPAAT LMLGQARGAC MTALVLKDLP
110 120 130 140 150
VAEYTALQVK QSVVGQGKAP KEQVQYMVVR MLNLSGTPQA DAADALAVAL
160 170
THANHSGGAI GKLALRGLKV RGGRLV
Length:176
Mass (Da):18,518
Last modified:December 15, 2003 - v1
Checksum:iF9011F191CB982E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016825 Genomic DNA. Translation: AAQ61885.1.
RefSeqiWP_011137771.1. NC_005085.1.

Genome annotation databases

EnsemblBacteriaiAAQ61885; AAQ61885; CV_4225.
GeneIDi24949006.
KEGGicvi:CV_4225.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRUVC_CHRVO
AccessioniPrimary (citable) accession number: Q7NQB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: December 15, 2003
Last modified: June 7, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families