Reviewed, UniProtKB/Swiss-Prot Q7NQ65 (GSHB_CHRVO)

Names and origin

Protein names

Recommended name:
    Glutathione synthetase
    EC=6.3.2.3
Alternative name(s):
    Glutathione synthase
    GSH synthetase
      Short name=GSH-S
      Short name=GSHase

Gene names

Name:	gshB
Ordered Locus Names:	CV_4275

Organism

Chromobacterium violaceum [Complete proteome] [HAMAP]

Taxonomic identifier

536 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Chromobacterium

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Glutathione synthetase HAMAP MF_00162		PRO_0000197463
Regions
	Domain	122 – 309	188	ATP-grasp
	Nucleotide binding	149 – 206	58	ATP By similarity
Sites
	Metal binding	280	1	Magnesium or manganese By similarity
	Metal binding	282	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NQ65-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: CAEB175EC4C135E2	FASTA	314	35,087
	10 20 30 40 50 60 MRILFIADPL EHFKIYKDTT FAMMEEAHRR GHAISFAEAH QLSVEGGRLQ VDACDLAVTD 70 80 90 100 110 120 ERPDWYKLGP KLRQPLTAFS AVVMRKDPPF DMEYLYASQL FTLAEAQGVK VFTSGQALRD 130 140 150 160 170 180 FNEKLAILHF PELISPTLIS GEAHRLRAFV NQHQDVILKP LDSMGGDSIF RVKPDDPNLS 190 200 210 220 230 240 VIIETITWRG KRTVMAQRYI PEIRDGDKRI LVIDGVPVDH CLARIPAAGE TRGNLAAGGR 250 260 270 280 290 300 GEARPLSARD REIAETVGPE LKKRGILLAG LDVIGNYLTE VNVTSPTCFR EIMDQTGVNV 310 AGLYIDALER RCQH

References

[1]

"The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability." Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T., Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R., Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F., Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S., Belo A. , van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M., Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P., Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S., Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N., Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P., Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R., Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B., Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J., Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F., Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z., de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F., Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A., Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C., Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M., Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C., Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R., Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M., Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R., Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G. Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003) [PubMed: 14500782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12472 / DSM 30191 / IFO 12614 / JCM 1249 / NCIB 9131.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016825 Genomic DNA. Translation: AAQ61935.1.
RefSeq	NP_903945.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2548852.
GenomeReviews	Gene locus CV_4275 in contig AE016825_GR.
KEGG	cvi:CV_4275.
NMPDR	fig|243365.1.peg.4275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NQ65.
OMA	Q7NQ65. VRNAPEK.
Enzyme and pathway databases
BioCyc	CVIO243365:CV_4275-MON.
BRENDA	6.3.2.3. 415.
Family and domain databases
HAMAP	MF_00162. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP_bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_CHRVO

Accession

Primary (citable) accession number: Q7NQ65

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)