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Protein

Glutamate 5-kinase

Gene

proB

Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71ATPUniRule annotation
Binding sitei48 – 481SubstrateUniRule annotation
Binding sitei135 – 1351SubstrateUniRule annotation
Binding sitei147 – 1471Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi167 – 1682ATPUniRule annotation
Nucleotide bindingi208 – 2147ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
  3. RNA binding Source: InterPro

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGVIO251221:GH9A-69-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:gll0069
OrganismiGloeobacter violaceus (strain PCC 7421)
Taxonomic identifieri251221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter
ProteomesiUP000000557 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Glutamate 5-kinasePRO_0000109676Add
BLAST

Proteomic databases

PRIDEiQ7NPI6.

Interactioni

Protein-protein interaction databases

STRINGi251221.gvip004.

Structurei

3D structure databases

ProteinModelPortaliQ7NPI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini275 – 35379PUAUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation
Contains 1 PUA domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
InParanoidiQ7NPI6.
KOiK00931.
OMAiVIAWGRE.
OrthoDBiEOG6PGK7G.
PhylomeDBiQ7NPI6.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NPI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVALVVKIGT SSLSDPSTGD LRLATLGGLA ETLTRLRRAG HRIILVSSGA
60 70 80 90 100
VGVGCARLGL KERPATVAGK QAAAAVGQGL LMSMYDRLFG ALGQPVAQVL
110 120 130 140 150
LTRQDLMDRV RYLNARETLS ELWRLGTVPI VNENDTVATD ELRFGDNDAL
160 170 180 190 200
SALVAGLVEA QWLVLLTDVA GLYSANPRLD PQARLLSEVT EISEELLQSA
210 220 230 240 250
RGRSLWGSGG MASKLEAARI AASAGVATVI TEGNTPQNIE RILAGEAIGT
260 270 280 290 300
RFALARPGGR LSLRKRWIGY GLVPAGALHL DEGAVLAVRE GGKSLLPAGV
310 320 330 340 350
RGVEGRFETG ALVRLIDGQG LEFARGLVNY SSEELEKIRG RKSHEIAALL
360
GIEGQPPTAV HRDNLVTLS
Length:369
Mass (Da):38,985
Last modified:December 15, 2003 - v1
Checksum:i98B51CA99F8DC482
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC88010.1.
RefSeqiNP_923015.1. NC_005125.1.

Genome annotation databases

EnsemblBacteriaiBAC88010; BAC88010; BAC88010.
GeneIDi2600046.
KEGGigvi:gvip004.
PATRICi22039487. VBIGloVio86258_0072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC88010.1.
RefSeqiNP_923015.1. NC_005125.1.

3D structure databases

ProteinModelPortaliQ7NPI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi251221.gvip004.

Proteomic databases

PRIDEiQ7NPI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC88010; BAC88010; BAC88010.
GeneIDi2600046.
KEGGigvi:gvip004.
PATRICi22039487. VBIGloVio86258_0072.

Phylogenomic databases

eggNOGiCOG0263.
InParanoidiQ7NPI6.
KOiK00931.
OMAiVIAWGRE.
OrthoDBiEOG6PGK7G.
PhylomeDBiQ7NPI6.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciGVIO251221:GH9A-69-MONOMER.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7421.

Entry informationi

Entry nameiPROB_GLOVI
AccessioniPrimary (citable) accession number: Q7NPI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 15, 2003
Last modified: March 4, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.