Q7NN66 (ARGD_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylornithine aminotransferase
Short name=ACOAT
EC=2.6.1.11

Gene names

Name:	argD
Ordered Locus Names:	glr0547

Organism

Gloeobacter violaceus (strain PCC 7421)

Taxonomic identifier

251221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate. HAMAP MF_01107
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP MF_01107
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4. HAMAP MF_01107
Subunit structure	Homodimer By similarity. HAMAP MF_01107
Subcellular location	Cytoplasm Probable HAMAP MF_01107.
Miscellaneous	May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis. HAMAP MF_01107
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.
Sequence caution	The sequence BAC88488.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Acetylornithine aminotransferase HAMAP MF_01107		PRO_0000112746
Regions
	Region	220 – 223	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	135	1	Pyridoxal phosphate; via carbonyl oxygen By similarity
	Binding site	138	1	N2-acetyl-L-ornithine By similarity
	Binding site	276	1	N2-acetyl-L-ornithine By similarity
	Binding site	277	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	249	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NN66 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: EEA5DC2136E8ECB1	FASTA	400	43,189
	10 20 30 40 50 60 MTVQQAFEQH VMHTYARFSV VFERGEGCYL EDSEGRRYLD FVAGIATCVL GHAHPVLSAA 70 80 90 100 110 120 VAEQARTLIH VSNLYYTPQQ ACLAEWLTAH SAADQVFFCN SGAEANEGAI KLARKYGRTV 130 140 150 160 170 180 LGIAEPQIIC AHQSFHGRTM ATVTATGQPK YQKHFHPLVP GFVHVPYNDF EALRAQVTDA 190 200 210 220 230 240 TAAVLIEPIQ GEGGVVPGDV EFFQKLRRFC SERRILLMLD EVQTGMGRTG RLFGYEHLGI 250 260 270 280 290 300 EPDVFTLAKA LGGGVPIGAL CAKEAFAIFE PGDHASTFGG NPLACAAALA VCQTLEAEQL 310 320 330 340 350 360 VDNARERGAQ LAAGLGRLVE RFKPLVRTAR GRGLMQGLVL SEPRAAEIVR LAMEQGLLLV 370 380 390 400 SAGPEVIRFV PPLIVSAIEV DEALAILEGV FARLPVTVTA

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000045 Genomic DNA. Translation: BAC88488.1. Different initiation.
RefSeq	NP_923493.1. NC_005125.1.
3D structure databases
ProteinModelPortal	Q7NN66.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2599568.
GenomeReviews	Gene locus glr0547 in contig BA000045_GR.
NMPDR	fig|251221.1.peg.547.
PATRIC	22040463. VBIGloVio86258_0556.
Phylogenomic databases
HOGENOM	HBG725944.
PhylomeDB	Q7NN66.
ProtClustDB	PRK02627.
Enzyme and pathway databases
BioCyc	GVIO251221:GLR0547-MONOMER.
Family and domain databases
HAMAP	MF_01107. ArgD_aminotrans_3. [Tree]
InterPro	IPR004636. AcOrn/SuccinylOrn_aminoTrfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF19. ArgD_aminotrans. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00707. ArgD. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGD_GLOVI

Accession

Primary (citable) accession number: Q7NN66

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	May 5, 2009
Last modified:	January 25, 2012
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program