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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Ordered Locus Names:glr1465
OrganismiGloeobacter violaceus (strain PCC 7421)
Taxonomic identifieri251221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacteraceaeGloeobacter
Proteomesi
  • UP000000557 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001828651 – 147Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST147

Interactioni

Protein-protein interaction databases

STRINGi251221.glr1465.

Structurei

3D structure databases

ProteinModelPortaliQ7NKL2.
SMRiQ7NKL2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 69Substrate bindingUniRule annotation3
Regioni84 – 86Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107ZFB. Bacteria.
COG0756. LUCA.
HOGENOMiHOG000028968.
InParanoidiQ7NKL2.
KOiK01520.
OrthoDBiPOG091H01I6.
PhylomeDBiQ7NKL2.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
HAMAPiMF_00116. dUTPase_bact. 1 hit.
InterProiView protein in InterPro
IPR029054. dUTPase-like.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
PfamiView protein in Pfam
PF00692. dUTPase. 1 hit.
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7NKL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPVRVAIQR LAHCFALPTC AHPGDAGLDL FAAHAVPLSI APGRFTRVPT
60 70 80 90 100
GIALGLPAGY MAFVQPRSGL AARHGISVLN TPGLIDCGYR GEIQVLLINH
110 120 130 140
GEVPVVVSRG DRIAQLVVLP VPQVQFVEVS TLESSERQTG SFGSSGY
Length:147
Mass (Da):15,466
Last modified:June 7, 2004 - v2
Checksum:i27730B315D71B34C
GO

Sequence cautioni

The sequence BAC89406 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC89406.1. Different initiation.
RefSeqiNP_924411.1. NC_005125.1.

Genome annotation databases

EnsemblBacteriaiBAC89406; BAC89406; BAC89406.
GeneIDi2598643.
KEGGigvi:glr1465.
PATRICifig|251221.4.peg.1497.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDUT_GLOVI
AccessioniPrimary (citable) accession number: Q7NKL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: July 5, 2017
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families