Reviewed, UniProtKB/Swiss-Prot Q7NJY2 (PIMT_GLOVI)

Names and origin

Protein names

Recommended name:
    Protein-L-isoaspartate O-methyltransferase
    EC=2.1.1.77
Alternative name(s):
    Protein-beta-aspartate methyltransferase
      Short name=PIMT
    Protein L-isoaspartyl methyltransferase
    L-isoaspartyl protein carboxyl methyltransferase

Gene names

Name:	pcm
Ordered Locus Names:	glr1699

Organism

Gloeobacter violaceus [Complete proteome] [HAMAP]

Taxonomic identifier

33072 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP MF_00090
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: HAMAP protein repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Protein-L-isoaspartate O-methyltransferase HAMAP MF_00090		PRO_0000111891
Sites
	Active site	52	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NJY2-1 [UniParc]. Last modified November 9, 2004. Version 2. Checksum: 9BD86ACFDF33FCF7	FASTA	205	22,643
	10 20 30 40 50 60 MVDEQLRPRG VEAQAVLAAM AKVPRHRFVP PPYTRLAYED RPLPIGHSQT ISQPFIVAYM 70 80 90 100 110 120 SEAARITPGA KVLEIGTGSG YQAAVLAEMG AEVYTVEIVP ELAKRAERTL EELGYRSVRV 130 140 150 160 170 180 RSGDGYQGWP QHAPFDAIVV TAAPERIPQP LIDQLAVNGR LIVPVGTQTE DQRMTVLTRT 190 200 PGGIVEQKTF PVRFVPLTRE KPQEH

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000045 Genomic DNA. Translation: BAC89640.1. Different initiation.
RefSeq	NP_924645.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2598479.
GenomeReviews	Gene locus glr1699 in contig BA000045_GR.
KEGG	gvi:glr1699.
NMPDR	fig|251221.1.peg.1699.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NJY2.
Enzyme and pathway databases
BioCyc	GVIO251221:GLR1699-MON.
BRENDA	2.1.1.77. 276183.
Family and domain databases
HAMAP	MF_00090. [Tree]
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_GLOVI

Accession

Primary (citable) accession number: Q7NJY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	November 9, 2004
Last modified:	June 16, 2009
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)