Reviewed, UniProtKB/Swiss-Prot Q7NJV3 (DEF1_GLOVI)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 1
      Short name=PDF 1
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 1

Gene names

Name:	def1
Ordered Locus Names:	glr1729

Organism

Gloeobacter violaceus [Complete proteome] [HAMAP]

Taxonomic identifier

33072 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Peptide deformylase 1 HAMAP MF_00163		PRO_0000082785
Sites
	Active site	196	1	By similarity
	Metal binding	153	1	Iron By similarity
	Metal binding	195	1	Iron By similarity
	Metal binding	199	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NJV3-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: CB0A4FE000DC1551	FASTA	227	25,170
	10 20 30 40 50 60 MHLKRAYPKD CLSGVGSNAC RIGDRQSRYP VTIECESTGT SGGGVYEIVK TGDPVLRLTA 70 80 90 100 110 120 KPLNSDEIQS EAIQQLIAAM AERMREAPGV GLAAPQVGVS VQLVVIEDRP EYIERLSGAE 130 140 150 160 170 180 RREREREPVP FHVLINPVLS VEGEESAVFF EGCLSIPGYQ GLVARARVVR VEALDERAAP 190 200 210 220 VVIRAHGWYA RILQHEIDHL NGLLCVDRMD LQTFSTLENY DRFWRGG

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000045 Genomic DNA. Translation: BAC89670.1. Different initiation.
RefSeq	NP_924675.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2600131.
GenomeReviews	Gene locus glr1729 in contig BA000045_GR.
KEGG	gvi:glr1729.
NMPDR	fig|251221.1.peg.1729.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NJV3.
Enzyme and pathway databases
BioCyc	GVIO251221:GLR1729-MON.
BRENDA	3.5.1.88. 276183.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF1_GLOVI

Accession

Primary (citable) accession number: Q7NJV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)