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Q7NIM7

- RBL_GLOVI

UniProt

Q7NIM7 - RBL_GLOVI

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Protein
Ribulose bisphosphate carboxylase large chain
Gene
cbbL, rbcL, glr2156
Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site By similarity.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221Substrate; in homodimeric partner By similarity
Binding sitei172 – 1721Substrate By similarity
Active sitei174 – 1741Proton acceptor By similarity
Binding sitei176 – 1761Substrate By similarity
Metal bindingi200 – 2001Magnesium; via carbamate group By similarity
Metal bindingi202 – 2021Magnesium By similarity
Metal bindingi203 – 2031Magnesium By similarity
Active sitei293 – 2931Proton acceptor By similarity
Binding sitei294 – 2941Substrate By similarity
Binding sitei326 – 3261Substrate By similarity
Sitei333 – 3331Transition state stabilizer By similarity
Binding sitei378 – 3781Substrate By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. monooxygenase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. photorespiration Source: UniProtKB-KW
  2. reductive pentose-phosphate cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Calvin cycle, Carbon dioxide fixation, Photorespiration, Photosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciGVIO251221:GH9A-2182-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chain (EC:4.1.1.39)
Short name:
RuBisCO large subunit
Gene namesi
Name:cbbL
Synonyms:rbcL
Ordered Locus Names:glr2156
OrganismiGloeobacter violaceus (strain PCC 7421)
Taxonomic identifieri251221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter
ProteomesiUP000000557: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Ribulose bisphosphate carboxylase large chainUniRule annotation
PRO_0000062623Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei200 – 2001N6-carboxylysine By similarity
Disulfide bondi246 – 246Interchain; in linked form By similarity

Post-translational modificationi

The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover By similarity.UniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ7NIM7.

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers By similarity.

Protein-protein interaction databases

STRINGi251221.gvip295.

Structurei

3D structure databases

ProteinModelPortaliQ7NIM7.
SMRiQ7NIM7. Positions 8-474.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1850.
KOiK01601.
OMAiFTQDWAS.
OrthoDBiEOG6ZKXMS.
PhylomeDBiQ7NIM7.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NIM7-1 [UniParc]FASTAAdd to Basket

« Hide

MSYTKTQAKA GYQAGVKDYR LTYYTPDYTP KDTDVLAAFR VTPQPGVPIE    50
EAGAAVAAES STGTWTTVWT DGLTELDRYK GRCYDIEPVP GEDNQWICYI 100
AYPLDLFEEG SVTNVLTSLV GNVFGFKALR ALRLEDIRFP IALVKTYQGP 150
PHGIVVERDK INKYGRPLLG CTIKPKLGLS AKNYGRAVYE CLRGGLDFTK 200
DDENINSQPF MRWRDRFLFV QDAIVKSQAE TGEIKGHYLN CTAGTCEEMM 250
ERAEFAKELK TPIIMHDYLT GGFTANTTLA KWCRRNGILL HIHRAMHAVI 300
DRQKNHGIHF RVLAKCLRLS GGDHIHTGTV VGKLEGERAS TMGFVDLLRE 350
EHVERDLSRG IYFTQDWASM PGVMAVASGG IHVWHMPALL DIFGDDAVLQ 400
FGGGTLGHPW GNAPGATANR VALEACVKAR NEGRDLMREA GDIIREAARW 450
SPELAAACEL WKEIKFEYEA VDKL 474
Length:474
Mass (Da):52,855
Last modified:December 15, 2003 - v1
Checksum:i6474FEF981055201
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000045 Genomic DNA. Translation: BAC90097.1.
RefSeqiNP_925102.1. NC_005125.1.

Genome annotation databases

EnsemblBacteriaiBAC90097; BAC90097; BAC90097.
GeneIDi2600557.
KEGGigvi:gvip295.
PATRICi22043775. VBIGloVio86258_2190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000045 Genomic DNA. Translation: BAC90097.1 .
RefSeqi NP_925102.1. NC_005125.1.

3D structure databases

ProteinModelPortali Q7NIM7.
SMRi Q7NIM7. Positions 8-474.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 251221.gvip295.

Proteomic databases

PRIDEi Q7NIM7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC90097 ; BAC90097 ; BAC90097 .
GeneIDi 2600557.
KEGGi gvi:gvip295.
PATRICi 22043775. VBIGloVio86258_2190.

Phylogenomic databases

eggNOGi COG1850.
KOi K01601.
OMAi FTQDWAS.
OrthoDBi EOG6ZKXMS.
PhylomeDBi Q7NIM7.

Enzyme and pathway databases

BioCyci GVIO251221:GH9A-2182-MONOMER.

Family and domain databases

Gene3Di 3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPi MF_01338. RuBisCO_L_type1.
InterProi IPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view ]
Pfami PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7421.

Entry informationi

Entry nameiRBL_GLOVI
AccessioniPrimary (citable) accession number: Q7NIM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: December 15, 2003
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi