Q7NIF5 (DEF2_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide deformylase 2
Short name=PDF 2
EC=3.5.1.88

Alternative name(s):
Polypeptide deformylase 2

Gene names

Name:	def2
Ordered Locus Names:	gll2228

Organism

Gloeobacter violaceus (strain PCC 7421)

Taxonomic identifier

251221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Peptide deformylase 2 HAMAP MF_00163		PRO_0000082786
Sites
	Active site	150	1	By similarity
	Metal binding	107	1	Iron By similarity
	Metal binding	149	1	Iron By similarity
	Metal binding	153	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NIF5 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 639E147377AAD007	FASTA	187	20,557
	10 20 30 40 50 60 MANQLQVPKQ KLAKPPLAIH TLGDRVLRQG SKQISGINDE VRKLAQQMLQ TMYSADGIGL 70 80 90 100 110 120 AAPQVGVNKR MIVVDIDPEN AARPPLVLIN PLIKQFSSDL AVDQEGCLSV PSIYADVRRP 130 140 150 160 170 180 ERVVATYRDL NGRPVTLEAT GLLARCIQHE IDHLDGVLFV DRVENQIALA PQLVEKGFAV RDVQVRA

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000045 Genomic DNA. Translation: BAC90169.1.
RefSeq	NP_925174.1. NC_005125.1.
3D structure databases
ProteinModelPortal	Q7NIF5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2600630.
GenomeReviews	Gene locus gll2228 in contig BA000045_GR.
KEGG	gvi:gll2228.
NMPDR	fig|251221.1.peg.2228.
PATRIC	22043915. VBIGloVio86258_2260.
Phylogenomic databases
HOGENOM	HBG665227.
OMA	RITKVDE.
PhylomeDB	Q7NIF5.
ProtClustDB	PRK00150.
Enzyme and pathway databases
BioCyc	GVIO251221:GLL2228-MONOMER.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. Pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF2_GLOVI

Accession

Primary (citable) accession number: Q7NIF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program