Q7NI42 (GATB_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	gll2342

Organism

Gloeobacter violaceus (strain PCC 7421) [Reference proteome] [HAMAP]

Taxonomic identifier

251221 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter › Gloeobacter violaceus

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_0000148792

Sequences

	Sequence		Length	Mass (Da)
	Q7NI42 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: B560F2D705995584	FASTA	554	60,533
	10 20 30 40 50 60 MTSTAPPKVQ YEAVIGLEVH VQLSTETKLF CRCSTRFGNT PNTNICPICT GQPGTLPVLN 70 80 90 100 110 120 QQALDYAVLT ASALNCQIHP QGLSKFDRKQ YFYPDLPKNY QISQYDLPLA ERGWLEIEVE 130 140 150 160 170 180 GEPAKRIGIT RLHMEEDAGK LVHAGADRLS GSTHSLVDFN RAGVALCEIV SEPDIRTAAE 190 200 210 220 230 240 AAAYAGELRR IVRYLGVCDG NMQEGSLRFD LNISVRPAGE GKFGTKVEIK NLNSFNSLQR 250 260 270 280 290 300 AVEYEFARQV DCLLSGERIV QETRLWDEAT QRTISMRSKE EANDYRYFPE PDLVPIALNG 310 320 330 340 350 360 TQIDAYRQRL GELPAQKRHR YRETLGLSSY DAGVLTDERE VAEYFEQVVA LGIPAKQAAN 370 380 390 400 410 420 FVSGAVAAHL NETRRSISQI KVTPEVSAEL LALINQGIIS NRIANELLPD LFEKGGSPRA 430 440 450 460 470 480 LVEERGLTQI SDRGQLEQIV DEVLAGESDS VAAYRGGRTK LLGFFVGKVM KKTAGRADPQ 490 500 510 520 530 540 VVNDLLQSKL AEQPTAPPPE PESAAETPEA PPAVEDAPPE APTEAITAEA GSAEAITAAS 550 EEPDTPVSHQ DAHA

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000045 Genomic DNA. Translation: BAC90283.1.
RefSeq	NP_925288.1. NC_005125.1.
3D structure databases
ProteinModelPortal	Q7NI42.
SMR	Q7NI42. Positions 10-417.
ModBase	Search...
Protein-protein interaction databases
STRING	251221.gll2342.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC90283; BAC90283; BAC90283.
GeneID	2600743.
KEGG	gvi:gll2342.
PATRIC	22044159. VBIGloVio86258_2380.
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223742.
KO	K02434.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Enzyme and pathway databases
BioCyc	GVIO251221:GH9A-2391-MONOMER.
Family and domain databases
Gene3D	1.10.10.410. 1 hit.
HAMAP	MF_00121. GatB. Divergent sequence.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR023168. GatB_Yqey_C. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_GLOVI

Accession

Primary (citable) accession number: Q7NI42

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program