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Q7NI06 (SYE_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:gll2378
OrganismGloeobacter violaceus (strain PCC 7421) [Reference proteome] [HAMAP]
Taxonomic identifier251221 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 530530Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119568

Regions

Motif26 – 3611"HIGH" region HAMAP-Rule MF_00022
Motif267 – 2715"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2701ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NI06 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: B9F8B474FD8F7FF9

FASTA53059,558
        10         20         30         40         50         60 
MNFEQYESAY PPRALPPGAW VTRIGPSPTG KAHIGTALQA VINRSLADGS GGAFLLRIED 

        70         80         90        100        110        120 
TDRERFVEGA IEDLLAALDW LGIRPDESVA HGGAYGPYVQ SERLPIYQAA ARQLVEVGHA 

       130        140        150        160        170        180 
YYCFCTPERL ESVRMAQQAA GKPTMYDRHC RNLGREEVEK RLATGEKAVV RLKVPDGTEI 

       190        200        210        220        230        240 
AFDDLARGRI AFESQTIDDS VLLKSDGFPT YHLAVVVDDH LMRVNQIIRG EEWIPSTPKH 

       250        260        270        280        290        300 
VLLYQYFGWP MPRIAHTPIL RDMSRRKLSK RSGDTSITGY RVQGYLPEGL RNFLTRIIWP 

       310        320        330        340        350        360 
HPEGKDVYDH EEFVRLFKAE DLPNTGPIVD PQLLDFINGQ YLRRLSSAEL YTATVEYLKF 

       370        380        390        400        410        420 
LLGLHQDIIF ETYHPSAPIK QTLTRAELGS FYSAFTANPT YTERVLTLEP ERYKRLSDIV 

       430        440        450        460        470        480 
VSTGFFFPAL FTPADPKLLA KPLGSPEKAG ALLGEYLRLY RPDAAQAEWE QSVRELAHEA 

       490        500        510        520        530 
GVKDGKLFMT LRVAVTGREQ TPPLFEVLTI LGGDEVQRRL ELAAAMPVSL 

« Hide

References

[1]"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids."
Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S.
DNA Res. 10:137-145(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7421.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000045 Genomic DNA. Translation: BAC90319.1.
RefSeqNP_925324.1. NC_005125.1.

3D structure databases

ProteinModelPortalQ7NI06.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING251221.gvip330.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC90319; BAC90319; BAC90319.
GeneID2600779.
KEGGgvi:gvip330.
PATRIC22044233. VBIGloVio86258_2417.

Phylogenomic databases

eggNOGCOG0008.
KOK01885.
OMAAFEGPYF.
OrthoDBEOG6DRPF7.
PhylomeDBQ7NI06.

Enzyme and pathway databases

BioCycGVIO251221:GH9A-2407-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 2 hits.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_GLOVI
AccessionPrimary (citable) accession number: Q7NI06
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries