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Q7NHJ3 (MDH_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:gll2542
OrganismGloeobacter violaceus (strain PCC 7421) [Reference proteome] [HAMAP]
Taxonomic identifier251221 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000113454

Regions

Nucleotide binding15 – 206NAD By similarity
Nucleotide binding124 – 1263NAD By similarity

Sites

Active site1811Proton acceptor By similarity
Binding site391NAD By similarity
Binding site881Substrate By similarity
Binding site941Substrate By similarity
Binding site1011NAD By similarity
Binding site1261Substrate By similarity
Binding site1571Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NHJ3 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: D7F5F1DB4253BFEC

FASTA32534,685
        10         20         30         40         50         60 
MAARSCRESK VSILGAGNVG SALAQRLIQG NVADVVLLDI VEGRPQGITL DLLEACGVEG 

        70         80         90        100        110        120 
HTCRITGTND YAQTAGSDVL VVAAGFARQP GMSRDDLLLT NTRIVFEVTQ KAVAHSPEAT 

       130        140        150        160        170        180 
VVVVTNPLDA MSHVAWRASG LVPERVMGMA GVLDAARFET FIAWELGFSV RDIRAMVLGG 

       190        200        210        220        230        240 
HGDLMVPLPR YTTISGVPLT QLLSSARIDA LIERTRTGGA EIVHLLKRGG AYYAPAAAAA 

       250        260        270        280        290        300 
RMIETLLGDE RRLLPVAAYL SGEYGLRNIH MGVPVILSRH GVERVVELTL ETEEKAALYR 

       310        320 
SAHTIRASLD QIAHLMPANQ PPSAV 

« Hide

References

[1]"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids."
Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S.
DNA Res. 10:137-145(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7421.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000045 Genomic DNA. Translation: BAC90483.1.
RefSeqNP_925488.1. NC_005125.1.

3D structure databases

ProteinModelPortalQ7NHJ3.
SMRQ7NHJ3. Positions 9-312.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING251221.gll2542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC90483; BAC90483; BAC90483.
GeneID2600943.
KEGGgvi:gll2542.
PATRIC22044565. VBIGloVio86258_2580.

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMAFVGVPVI.
OrthoDBEOG6091FG.

Enzyme and pathway databases

BioCycGVIO251221:GH9A-2574-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_GLOVI
AccessionPrimary (citable) accession number: Q7NHJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 15, 2003
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families