Q7NGW6 (KATG_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	gll2771

Organism

Gloeobacter violaceus (strain PCC 7421)

Taxonomic identifier

251221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Catalase-peroxidase HAMAP MF_01961		PRO_0000354803
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	261	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 220		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-246) By similarity
	Cross-link	220 ↔ 246		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NGW6 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 18F3B16163E81E7F	FASTA	735	80,389
	10 20 30 40 50 60 MDVEAKCPMG GGKVEGRAVL FEMTNRLWWP NHLDLSVLHQ NPPAGNPMGE GFNYAAEFES 70 80 90 100 110 120 LDLAAVKQDI FALMTESQDW WPADYGHYGP LFIRMAWHAA GTYRIGDGRG GAGAGTQRFA 130 140 150 160 170 180 PLNSWPDNAN LDKARLLLWP IKEKYGRKLS WGDLLILAGN CALESMGFKT AGFAGGRVDI 190 200 210 220 230 240 WEPENDIFWG PEREWLGDER YSSDRDLAHP LAAVQMGLIY VNPEGPNGKP DPMAAAHDIR 250 260 270 280 290 300 ETFGRMAMND EETVALIAGG HTFGKCHGAA EPSQYVGAEP EGAGIERQGL GWDNSFGSGR 310 320 330 340 350 360 GVHTITSGLE GAWTKNPIQW DNGYFENLFE YEWELTKSPA GAHQWTPKNP EAASTVPDAH 370 380 390 400 410 420 DPHKRHAPMM ATTDLAMRAD PAYEKISRRF YDNPDQLAHA FAEAWYKLTH RDMGPYARLL 430 440 450 460 470 480 GPEVPSEPRI WQDPVPASDH PLIDDADIAE LKAQILAAGL SIARLVTTAW ASASTFRGTD 490 500 510 520 530 540 KRGGANGARI RLVPQKDWAV NEPTELATAL AKLEAIQQDF NAGQTGGKQV SLADLIVLGG 550 560 570 580 590 600 CAAVEQAAKK AGHDITVPFT PGRTDASPDQ TDVESFAPLE PKIDGFRNYV GEKSVYSAEE 610 620 630 640 650 660 MLVDRAHLLT LSAPEMTVLV GGLRVLGANY GGSKLGVFTE RPETLTNDFF VNLLKTSTSM 670 680 690 700 710 720 KWEASPDADV FEASDRATGE KKWAGTRVDL IFGSNSQLRA LSEAYATADA QQTFVDAFVA 730 AWTKVMNLDR FDLPR

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000045 Genomic DNA. Translation: BAC90712.1.
RefSeq	NP_925717.1. NC_005125.1.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ProteinModelPortal	Q7NGW6.
ModBase	Search...
Protein family/group databases
PeroxiBase	2378. GviCP01_PCC7421.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2601172.
GenomeReviews	Gene locus gll2771 in contig BA000045_GR.
KEGG	gvi:gll2771.
NMPDR	fig|251221.1.peg.2771.
PATRIC	22045009. VBIGloVio86258_2799.
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	Q7NGW6.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	GVIO251221:GLL2771-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_GLOVI

Accession

Primary (citable) accession number: Q7NGW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program