Q7NGU6 (ISPD_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	glr2791

Organism

Gloeobacter violaceus (strain PCC 7421)

Taxonomic identifier

251221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000075577
Sites
	Site	13	1	Transition state stabilizer By similarity
	Site	20	1	Transition state stabilizer By similarity
	Site	153	1	Positions MEP for the nucleophilic attack By similarity
	Site	209	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NGU6 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 05C265F0A72C14E9	FASTA	233	24,894
	10 20 30 40 50 60 MHLLIPAAGR GSRMGADGNK LLLPLSGRPL LAWTLAAAAQ APSTRWIGII GQPMDEEAIR 70 80 90 100 110 120 LLAQKLGLRV PVHWIEGGET RQQSVYRGLL ALPVEAQQVL IHDGARCLAS PALFERCAAA 130 140 150 160 170 180 LAEFSAIVAA VPVKDTIKQV YPQSRQVEKT IPREQLWAAQ TPQGGNVGRL KAAHAWAAAQ 190 200 210 220 230 AVAVTDDAAL CELRGEPVQV VPGEETNLKI TTPADLLVAE AILKTQLRQD PSP

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000045 Genomic DNA. Translation: BAC90732.1.
RefSeq	NP_925737.1. NC_005125.1.
3D structure databases
ProteinModelPortal	Q7NGU6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2601192.
GenomeReviews	Gene locus glr2791 in contig BA000045_GR.
KEGG	gvi:glr2791.
NMPDR	fig|251221.1.peg.2791.
PATRIC	22045051. VBIGloVio86258_2820.
Phylogenomic databases
HOGENOM	HBG672839.
OMA	SKVIVVC.
PhylomeDB	Q7NGU6.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	GVIO251221:GLR2791-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_GLOVI

Accession

Primary (citable) accession number: Q7NGU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program