Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151Carbamoyl phosphateBy similarity
Sitei36 – 361Important for structural integrityBy similarity
Binding sitei73 – 731Carbamoyl phosphateBy similarity
Binding sitei84 – 841Carbamoyl phosphateBy similarity
Binding sitei108 – 1081Carbamoyl phosphateBy similarity
Sitei148 – 1481Important for structural integrityBy similarity
Binding sitei166 – 1661OrnithineBy similarity
Binding sitei230 – 2301OrnithineBy similarity
Binding sitei280 – 2801Carbamoyl phosphateBy similarity
Binding sitei298 – 2981Carbamoyl phosphateBy similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. ornithine carbamoyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciGVIO251221:GH9A-3139-MONOMER.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:gll3101
OrganismiGloeobacter violaceus (strain PCC 7421)
Taxonomic identifieri251221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter
ProteomesiUP000000557: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Ornithine carbamoyltransferasePRO_0000112927Add
BLAST

Proteomic databases

PRIDEiQ7NGR7.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi251221.gvip429.

Structurei

Secondary structure

1
313
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 163Combined sources
Helixi17 – 193Combined sources
Helixi22 – 3615Combined sources
Beta strandi48 – 558Combined sources
Helixi58 – 7013Combined sources
Beta strandi74 – 785Combined sources
Helixi91 – 10111Combined sources
Beta strandi103 – 1086Combined sources
Helixi112 – 12211Combined sources
Beta strandi126 – 1305Combined sources
Helixi136 – 15015Combined sources
Beta strandi157 – 1626Combined sources
Helixi166 – 17712Combined sources
Beta strandi181 – 1855Combined sources
Helixi194 – 20714Combined sources
Beta strandi211 – 2155Combined sources
Helixi217 – 2215Combined sources
Beta strandi225 – 2295Combined sources
Helixi244 – 2485Combined sources
Helixi249 – 2513Combined sources
Helixi255 – 2584Combined sources
Beta strandi266 – 2694Combined sources
Turni276 – 2783Combined sources
Helixi281 – 2844Combined sources
Helixi291 – 31020Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GD5X-ray2.10A/B/C/D/E/F2-313[»]
ProteinModelPortaliQ7NGR7.
SMRiQ7NGR7. Positions 12-312.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7NGR7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 615Carbamoyl phosphate bindingBy similarity
Regioni135 – 1384Carbamoyl phosphate bindingBy similarity
Regioni234 – 2352Ornithine bindingBy similarity
Regioni269 – 2724Carbamoyl phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiCOG0078.
InParanoidiQ7NGR7.
KOiK00611.
OMAiGNNVCNS.
OrthoDBiEOG690MGV.
PhylomeDBiQ7NGR7.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NGR7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSASLGATRF RPDLLSLDDL DEAQLHALLT LAHQLKRGER VANLHGKVLG
60 70 80 90 100
LVFLKASTRT RVSFTVAMYQ LGGQVIDLSP SNTQVGRGEP VRDTARVLGR
110 120 130 140 150
YVDGLAIRTF AQTELEEYAH YAGIPVINAL TDHEHPCQVV ADLLTIRENF
160 170 180 190 200
GRLAGLKLAY VGDGNNVAHS LLLGCAKVGM SIAVATPEGF TPDPAVSARA
210 220 230 240 250
SEIAGRTGAE VQILRDPFEA ARGAHILYTD VWTSMGQEAE TQHRLQLFEQ
260 270 280 290 300
YQINAALLNC AAAEAIVLHC LPAHRGEEIT DEVMEGPRSR IWDEAENRLH
310
AQKAVLAALM GGR
Length:313
Mass (Da):33,958
Last modified:December 15, 2003 - v1
Checksum:i705948D76BE67854
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC91042.1.
RefSeqiNP_926047.1. NC_005125.1.
WP_011143094.1. NC_005125.1.

Genome annotation databases

EnsemblBacteriaiBAC91042; BAC91042; BAC91042.
GeneIDi2601502.
KEGGigvi:gvip429.
PATRICi22045673. VBIGloVio86258_3130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC91042.1.
RefSeqiNP_926047.1. NC_005125.1.
WP_011143094.1. NC_005125.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GD5X-ray2.10A/B/C/D/E/F2-313[»]
ProteinModelPortaliQ7NGR7.
SMRiQ7NGR7. Positions 12-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi251221.gvip429.

Proteomic databases

PRIDEiQ7NGR7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC91042; BAC91042; BAC91042.
GeneIDi2601502.
KEGGigvi:gvip429.
PATRICi22045673. VBIGloVio86258_3130.

Phylogenomic databases

eggNOGiCOG0078.
InParanoidiQ7NGR7.
KOiK00611.
OMAiGNNVCNS.
OrthoDBiEOG690MGV.
PhylomeDBiQ7NGR7.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
BioCyciGVIO251221:GH9A-3139-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ7NGR7.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7421.
  2. "Crystal structure of ornithine carbamoyltransferase from Gloeobacter violaceus."
    New york sgx research center for structural genomics (NYSGXRC)
    Fedorov A.A., Fedorov E.V., Toro R., Ramagopal U.A., Sauder J.M., Burley S.K., Almo S.C.
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiOTC_GLOVI
AccessioniPrimary (citable) accession number: Q7NGR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 15, 2003
Last modified: February 4, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.