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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15Carbamoyl phosphateBy similarity1
Binding sitei73Carbamoyl phosphateBy similarity1
Binding sitei84Carbamoyl phosphateBy similarity1
Binding sitei108Carbamoyl phosphateBy similarity1
Binding sitei166OrnithineBy similarity1
Binding sitei230OrnithineBy similarity1
Binding sitei280Carbamoyl phosphateBy similarity1
Binding sitei298Carbamoyl phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argF
Ordered Locus Names:gll3101
OrganismiGloeobacter violaceus (strain PCC 7421)
Taxonomic identifieri251221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacteraceaeGloeobacter
Proteomesi
  • UP000000557 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001129271 – 313Ornithine carbamoyltransferaseAdd BLAST313

Proteomic databases

PRIDEiQ7NGR7.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi251221.gvip429.

Structurei

Secondary structure

1313
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Helixi17 – 19Combined sources3
Helixi22 – 36Combined sources15
Beta strandi48 – 55Combined sources8
Helixi58 – 70Combined sources13
Beta strandi74 – 78Combined sources5
Helixi91 – 98Combined sources8
Turni99 – 101Combined sources3
Beta strandi103 – 108Combined sources6
Helixi112 – 122Combined sources11
Beta strandi126 – 130Combined sources5
Helixi136 – 150Combined sources15
Beta strandi157 – 162Combined sources6
Helixi166 – 177Combined sources12
Beta strandi181 – 185Combined sources5
Helixi194 – 207Combined sources14
Beta strandi211 – 215Combined sources5
Helixi217 – 221Combined sources5
Beta strandi225 – 229Combined sources5
Helixi245 – 248Combined sources4
Helixi255 – 259Combined sources5
Beta strandi266 – 269Combined sources4
Turni276 – 278Combined sources3
Helixi281 – 285Combined sources5
Helixi291 – 310Combined sources20

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei36Important for structural integrityBy similarity1
Sitei148Important for structural integrityBy similarity1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GD5X-ray2.10A/B/C/D/E/F2-313[»]
ProteinModelPortaliQ7NGR7.
SMRiQ7NGR7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7NGR7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 61Carbamoyl phosphate bindingBy similarity5
Regioni135 – 138Carbamoyl phosphate bindingBy similarity4
Regioni234 – 235Ornithine bindingBy similarity2
Regioni269 – 272Carbamoyl phosphate bindingBy similarity4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
InParanoidiQ7NGR7.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiPOG091H0543.
PhylomeDBiQ7NGR7.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NGR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASLGATRF RPDLLSLDDL DEAQLHALLT LAHQLKRGER VANLHGKVLG
60 70 80 90 100
LVFLKASTRT RVSFTVAMYQ LGGQVIDLSP SNTQVGRGEP VRDTARVLGR
110 120 130 140 150
YVDGLAIRTF AQTELEEYAH YAGIPVINAL TDHEHPCQVV ADLLTIRENF
160 170 180 190 200
GRLAGLKLAY VGDGNNVAHS LLLGCAKVGM SIAVATPEGF TPDPAVSARA
210 220 230 240 250
SEIAGRTGAE VQILRDPFEA ARGAHILYTD VWTSMGQEAE TQHRLQLFEQ
260 270 280 290 300
YQINAALLNC AAAEAIVLHC LPAHRGEEIT DEVMEGPRSR IWDEAENRLH
310
AQKAVLAALM GGR
Length:313
Mass (Da):33,958
Last modified:December 15, 2003 - v1
Checksum:i705948D76BE67854
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC91042.1.
RefSeqiNP_926047.1. NC_005125.1.
WP_011143094.1. NC_005125.1.

Genome annotation databases

EnsemblBacteriaiBAC91042; BAC91042; BAC91042.
GeneIDi2601502.
KEGGigvi:gvip429.
PATRICi22045673. VBIGloVio86258_3130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC91042.1.
RefSeqiNP_926047.1. NC_005125.1.
WP_011143094.1. NC_005125.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GD5X-ray2.10A/B/C/D/E/F2-313[»]
ProteinModelPortaliQ7NGR7.
SMRiQ7NGR7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi251221.gvip429.

Proteomic databases

PRIDEiQ7NGR7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC91042; BAC91042; BAC91042.
GeneIDi2601502.
KEGGigvi:gvip429.
PATRICi22045673. VBIGloVio86258_3130.

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
InParanoidiQ7NGR7.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiPOG091H0543.
PhylomeDBiQ7NGR7.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Miscellaneous databases

EvolutionaryTraceiQ7NGR7.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_GLOVI
AccessioniPrimary (citable) accession number: Q7NGR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 15, 2003
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.