Reviewed, UniProtKB/Swiss-Prot Q7NGI6 (SAHH_GLOVI)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	glr3183

Organism

Gloeobacter violaceus [Complete proteome] [HAMAP]

Taxonomic identifier

33072 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000116964
Regions
	Region	186 – 353	168	NAD binding By similarity
Sites
	Binding site	62	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity
	Binding site	193	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NGI6-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 55B8B09585998B9F	FASTA	428	46,150
	10 20 30 40 50 60 MTAVKTPIQV RYEVRNLELA AEGRQRIEWA AREMPVLKQL RQRFSQERPL AGIRISACCH 70 80 90 100 110 120 VTTETANLAV ALKAAGADAL LIASNPLSTQ DDVAASLVVD HGIPVFALKG EDTATYLRHV 130 140 150 160 170 180 RVALDHRPQI IIDDGSDVVA TMIKDRPDLI AGVIGTTEET TTGIVRLKAM LAAGVLTFPA 190 200 210 220 230 240 MAVNDAETKH FFDNRYGTGQ STLDGIIRAT NILLAGKTIV VAGYGWCGKG VALRARGMGA 250 260 270 280 290 300 NVVVTEINPV RAIEAAMDGL QVMPMAEAAC LGDLFITVTG NKHVIRREHF AMMKDGAIVC 310 320 330 340 350 360 NSGHFDIEID LVALGELSSE RRMVRPFTEE YTLEGGKSVI VLGEGRLINL AAAEGHPASV 370 380 390 400 410 420 MDMSFANQAL AVEYLIKNQG KLSPGIYNIP EELDRQIAAL KLAAMGIAID TLTPDQLHYI SSWDEGTE

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000045 Genomic DNA. Translation: BAC91124.1.
RefSeq	NP_926129.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2601584.
GenomeReviews	Gene locus glr3183 in contig BA000045_GR.
KEGG	gvi:glr3183.
NMPDR	fig|251221.1.peg.3183.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NGI6.
OMA	IDDGMDL.
Enzyme and pathway databases
BioCyc	GVIO251221:GLR3183-MON.
BRENDA	3.3.1.1. 276183.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_GLOVI

Accession

Primary (citable) accession number: Q7NGI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)