Q7NFL5 (BIOD_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	gll3509

Organism

Gloeobacter violaceus (strain PCC 7421)

Taxonomic identifier

251221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302512
Regions
	Nucleotide binding	105 – 108	4	ATP By similarity
	Nucleotide binding	165 – 166	2	ATP By similarity
Sites
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	46	1	Magnesium 2 By similarity
	Metal binding	105	1	Magnesium 2 By similarity
	Binding site	46	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NFL5 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 10B01E9C544168E3	FASTA	229	24,051
	10 20 30 40 50 60 MAGVLVSGTD TGAGKTILCA ALAAWWLAHR RTPAAILKPV QCGPGDREYY RTVFGDALSV 70 80 90 100 110 120 LNPLYFEAPL APPLAAAREG QTVDIGLLWK SYCEAAAGHA LVLVEGVGGL GCPLGWDYTV 130 140 150 160 170 180 ADLARDWRLP VLLVAPLRLG VVGQLVAHTG FARAQNLDLS ALVLSEIEPV SAEQRAQWAD 190 200 210 220 VQLIENLCHL PVLGVLSHLE AATDRAALAA AGSGLWLEAA GTLFTAERG

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000045 Genomic DNA. Translation: BAC91450.1.
RefSeq	NP_926455.1. NC_005125.1.
3D structure databases
ProteinModelPortal	Q7NFL5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2601913.
GenomeReviews	Gene locus gll3509 in contig BA000045_GR.
NMPDR	fig|251221.1.peg.3509.
PATRIC	22046509. VBIGloVio86258_3543.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	ALAAYWQ.
PhylomeDB	Q7NFL5.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	GVIO251221:GLL3509-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_GLOVI

Accession

Primary (citable) accession number: Q7NFL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program