Q7NFD3 (QUEF_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	gll3593

Organism

Gloeobacter violaceus (strain PCC 7421)

Taxonomic identifier

251221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm Probable HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_0000162973

Sequences

	Sequence		Length	Mass (Da)
	Q7NFD3 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: F2C2935E3002B293	FASTA	137	15,492
	10 20 30 40 50 60 MSDLPVAPTP AEPVKYGERA IEAGQLITFP NPRPGRDYDI HITLPEFTCK CPFSGYPDFA 70 80 90 100 110 120 TIYLTYVPHE KVVELKALKL YVNSFRDRYI SHEEVVHVVL DDFVAAADPL RVQIKGDFNP 130 RGNVHMVVEA RHTRPGT

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000045 Genomic DNA. Translation: BAC91534.1.
RefSeq	NP_926539.1. NC_005125.1.
3D structure databases
ProteinModelPortal	Q7NFD3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2601997.
GenomeReviews	Gene locus gll3593 in contig BA000045_GR.
KEGG	gvi:gll3593.
NMPDR	fig|251221.1.peg.3593.
PATRIC	22046681. VBIGloVio86258_3626.
Phylogenomic databases
HOGENOM	HBG294103.
OMA	FRNAGIF.
PhylomeDB	Q7NFD3.
Enzyme and pathway databases
BioCyc	GVIO251221:GLL3593-MONOMER.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_GLOVI

Accession

Primary (citable) accession number: Q7NFD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program