Reviewed, UniProtKB/Swiss-Prot Q7NFC3 (TRMFO_GLOVI)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Ordered Locus Names:	glr3603

Organism

Gloeobacter violaceus [Complete proteome] [HAMAP]

Taxonomic identifier

33072 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000346342
Regions
	Nucleotide binding	9 – 14	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NFC3-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 5A14D7453E407C59	FASTA	449	49,166
	10 20 30 40 50 60 MIEPVRVIGG GIAGAEAAWQ CARAGVPVVL SEMRPVRPSP AHHTDHLGEL VCSNSFGAMA 70 80 90 100 110 120 TDRAPGLLKE ELRRLGSLVI AVADAHAVPA GGALAVDRAV YTRELTERVA NHPLIELRRE 130 140 150 160 170 180 EVTEIPAAGL VVFATGPLTS EPLAHGLQAL TGLGYLSFFD AASPIVAGES LDTEKVFLAS 190 200 210 220 230 240 RYDRGEAAYY NCPMSEPEYR AFWEALASAE QAELKEFEHE ERKFFEACLP VEELARRGYE 250 260 270 280 290 300 TLRYGPLKPV GLTDPRTGRW PFACVQLRQE DRAGRLWNLV GFQTNLKWGE QKRVFQMIPG 310 320 330 340 350 360 LEQAEFVRLG VMHRNTFLCS PHLLAPTLQF HAHPRLLACG QLTGTEGYTA AVAGGWLAGT 370 380 390 400 410 420 NAARLAQSRD PLVLPVETMA GALVDYVSHG DPKTFQPMPP NFALLPEITP RIRHKQQRYW 430 440 AYRDRALAAI DTFGRTHGLG AAPALARAN

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000045 Genomic DNA. Translation: BAC91544.1. Different initiation.
RefSeq	NP_926549.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2602007.
GenomeReviews	Gene locus glr3603 in contig BA000045_GR.
KEGG	gvi:glr3603.
NMPDR	fig|251221.1.peg.3603.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NFC3.
Enzyme and pathway databases
BioCyc	GVIO251221:GLR3603-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_GLOVI

Accession

Primary (citable) accession number: Q7NFC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)