Q7NFA1 (ACSF_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	gll3625

Organism

Gloeobacter violaceus (strain PCC 7421)

Taxonomic identifier

251221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity. HAMAP MF_01840
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity. HAMAP MF_01840
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent). HAMAP MF_01840
Sequence similarities	Belongs to the AcsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_0000217529

Sequences

	Sequence		Length	Mass (Da)
	Q7NFA1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 60FA7605D5EB8749	FASTA	346	40,171
	10 20 30 40 50 60 MVDALREANT ASQSERRETL LTPRFYTTDF EALAKLDISA MEADFEALLA EFQADYNREH 70 80 90 100 110 120 FQRDAGFVEQ AHQIDPQWRA LMLEFLERSC TSEFSGFLLY KEISRRLKAA NPLLAECFKA 130 140 150 160 170 180 MSRDEARHAG FLNKAMGDYD LGLDLSNLAK QKKYTFFKPK FIFYATYLSE KIGYWRYIAI 190 200 210 220 230 240 FRHLERHPEN RIHPLFNYFK NWCQDENRHG DFFGLILKSQ PQLLKGPINR LWVRFFLLSF 250 260 270 280 290 300 CLTMYLNDRK PHNLYSALGL DASHYAMEVL RQTNLDSGKV FPLILDLDNP RFQRHLDRCA 310 320 330 340 DLNLQLEALS GKSPLARLPV IAALAGTIAQ LFLLKPVAPP APEMVR

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000045 Genomic DNA. Translation: BAC91566.1.
RefSeq	NP_926571.1. NC_005125.1.
3D structure databases
ProteinModelPortal	Q7NFA1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2602029.
GenomeReviews	Gene locus gll3625 in contig BA000045_GR.
NMPDR	fig|251221.1.peg.3625.
PATRIC	22046747. VBIGloVio86258_3659.
Phylogenomic databases
HOGENOM	HBG284843.
OMA	HGDFFDA.
PhylomeDB	Q7NFA1.
ProtClustDB	PRK13654.
Enzyme and pathway databases
BioCyc	GVIO251221:GLL3625-MONOMER.
Family and domain databases
HAMAP	MF_01840. AcsF. [Tree]
InterPro	IPR008434. AcsF. IPR012347. Ferritin-rel. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Gene3D	G3DSA:1.20.1260.10. Ferritin_rel. 1 hit.
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_GLOVI

Accession

Primary (citable) accession number: Q7NFA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program