SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q7NF75

- SYI_GLOVI

UniProt

Q7NF75 - SYI_GLOVI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene
ileS, gll3651
Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei608 – 6081Aminoacyl-adenylate By similarity
Binding sitei652 – 6521ATP By similarity
Metal bindingi952 – 9521Zinc By similarity
Metal bindingi955 – 9551Zinc By similarity
Metal bindingi972 – 9721Zinc By similarity
Metal bindingi975 – 9751Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciGVIO251221:GH9A-3700-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:gll3651
OrganismiGloeobacter violaceus (strain PCC 7421)
Taxonomic identifieri251221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter
ProteomesiUP000000557: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 983983Isoleucine--tRNA ligaseUniRule annotationPRO_0000098392Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi251221.gvip495.

Structurei

3D structure databases

ProteinModelPortaliQ7NF75.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionUniRule annotationAdd
BLAST
Motifi649 – 6535"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.
PhylomeDBiQ7NF75.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NF75-1 [UniParc]FASTAAdd to Basket

« Hide

MTQTPVDYKQ TVRTPKTDFP MRANASTREV EIQRFWEEQG IYGRLAQENP    50
GEIFILHDGP PYANGELHVG HALNKILKDI INRYQLLQGR RVRYVPGWDC 100
HGLPIELKVL QDLSQAERSQ LTPLDVRTRA RDFAQRTVQS QCASYKRFGV 150
WGDWDHPYLT LQPEYEAAQL GVFGKMALKG YIYRGLKPVH WSPSSQTALA 200
EAELEYPTKD DGSPAHTSRS VYVKFPLISI AAPETATVMA AELLPRLSAF 250
HDREEELIDA LLGERDDLPE IAVAIWTTTP WTLPGNLAVA LNGELDYALV 300
ASDEHGLLIV AAELVERLAG TLATRFETIA TFRGRELEGS LLAHPLFRRT 350
SPIVLGDHVT TESGTGAVHT APGHGSEDFE LAQRYNLGVL SPVDDYGRFT 400
READSDRREN LPVFAGKAVL SDGNQAVIEA LSAAGALLKE EAYVHKYPYD 450
WRTKKPTIFR ATTQWFASVS DFRPQALSAI AQTEWIPASG ENRITAMVAG 500
RNDWCISRQR AWGLPIPAFY CENCANVLLT QESVEAVQAA VRVHGSDIWW 550
QKEASELLPP GIACAHCGGT AFRKEKDIMD VWFDSGSSWA GVLGRRPELH 600
YPADVYLEGS DQHRGWFQSS LLTCVATEGT APYKTVITHG FTLDEHGRKM 650
SKSLGNVVDP KLVIDGGANQ KQHPAYGADV LRLWVASVDY TSDQLVGPSV 700
LAQIAEVYRK IRNTARYLLG SLNDFVPERD LVAFDSLGEV DQYLLHRLSV 750
VHLEVTQAFE SYQFSRFFQT IQNFCVADLS NFYLDISKDR LYISAEVSLR 800
RRSCQTVLYR VLESLTRLIA PVLPHLAEDI WQHLPYPRSD ASVFEAGWPV 850
DHSQWFQPLT VDRWPGLIVL RDRVNIALEA ARNAKRIGSS LEAKIRLHVE 900
DPALTDELAR QKDELRYLFI VSQVELLDEL PAEVSVEEGA AVIVLDADGQ 950
KCERCWNYSV HVGEDAEHPT LCERCVSALA GAF 983
Length:983
Mass (Da):110,014
Last modified:December 15, 2003 - v1
Checksum:i7F4A0F7C6DDD130C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000045 Genomic DNA. Translation: BAC91592.1.
RefSeqiNP_926597.1. NC_005125.1.
WP_011143640.1. NC_005125.1.

Genome annotation databases

EnsemblBacteriaiBAC91592; BAC91592; BAC91592.
GeneIDi2602055.
KEGGigvi:gvip495.
PATRICi22046801. VBIGloVio86258_3686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000045 Genomic DNA. Translation: BAC91592.1 .
RefSeqi NP_926597.1. NC_005125.1.
WP_011143640.1. NC_005125.1.

3D structure databases

ProteinModelPortali Q7NF75.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 251221.gvip495.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC91592 ; BAC91592 ; BAC91592 .
GeneIDi 2602055.
KEGGi gvi:gvip495.
PATRICi 22046801. VBIGloVio86258_3686.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.
PhylomeDBi Q7NF75.

Enzyme and pathway databases

BioCyci GVIO251221:GH9A-3700-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7421.

Entry informationi

Entry nameiSYI_GLOVI
AccessioniPrimary (citable) accession number: Q7NF75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi