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Q7NF75

- SYI_GLOVI

UniProt

Q7NF75 - SYI_GLOVI

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei608 – 6081Aminoacyl-adenylateUniRule annotation
    Binding sitei652 – 6521ATPUniRule annotation
    Metal bindingi952 – 9521ZincUniRule annotation
    Metal bindingi955 – 9551ZincUniRule annotation
    Metal bindingi972 – 9721ZincUniRule annotation
    Metal bindingi975 – 9751ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciGVIO251221:GH9A-3700-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:gll3651
    OrganismiGloeobacter violaceus (strain PCC 7421)
    Taxonomic identifieri251221 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter
    ProteomesiUP000000557: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 983983Isoleucine--tRNA ligasePRO_0000098392Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi251221.gvip495.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7NF75.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 7111"HIGH" regionAdd
    BLAST
    Motifi649 – 6535"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.
    PhylomeDBiQ7NF75.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7NF75-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQTPVDYKQ TVRTPKTDFP MRANASTREV EIQRFWEEQG IYGRLAQENP    50
    GEIFILHDGP PYANGELHVG HALNKILKDI INRYQLLQGR RVRYVPGWDC 100
    HGLPIELKVL QDLSQAERSQ LTPLDVRTRA RDFAQRTVQS QCASYKRFGV 150
    WGDWDHPYLT LQPEYEAAQL GVFGKMALKG YIYRGLKPVH WSPSSQTALA 200
    EAELEYPTKD DGSPAHTSRS VYVKFPLISI AAPETATVMA AELLPRLSAF 250
    HDREEELIDA LLGERDDLPE IAVAIWTTTP WTLPGNLAVA LNGELDYALV 300
    ASDEHGLLIV AAELVERLAG TLATRFETIA TFRGRELEGS LLAHPLFRRT 350
    SPIVLGDHVT TESGTGAVHT APGHGSEDFE LAQRYNLGVL SPVDDYGRFT 400
    READSDRREN LPVFAGKAVL SDGNQAVIEA LSAAGALLKE EAYVHKYPYD 450
    WRTKKPTIFR ATTQWFASVS DFRPQALSAI AQTEWIPASG ENRITAMVAG 500
    RNDWCISRQR AWGLPIPAFY CENCANVLLT QESVEAVQAA VRVHGSDIWW 550
    QKEASELLPP GIACAHCGGT AFRKEKDIMD VWFDSGSSWA GVLGRRPELH 600
    YPADVYLEGS DQHRGWFQSS LLTCVATEGT APYKTVITHG FTLDEHGRKM 650
    SKSLGNVVDP KLVIDGGANQ KQHPAYGADV LRLWVASVDY TSDQLVGPSV 700
    LAQIAEVYRK IRNTARYLLG SLNDFVPERD LVAFDSLGEV DQYLLHRLSV 750
    VHLEVTQAFE SYQFSRFFQT IQNFCVADLS NFYLDISKDR LYISAEVSLR 800
    RRSCQTVLYR VLESLTRLIA PVLPHLAEDI WQHLPYPRSD ASVFEAGWPV 850
    DHSQWFQPLT VDRWPGLIVL RDRVNIALEA ARNAKRIGSS LEAKIRLHVE 900
    DPALTDELAR QKDELRYLFI VSQVELLDEL PAEVSVEEGA AVIVLDADGQ 950
    KCERCWNYSV HVGEDAEHPT LCERCVSALA GAF 983
    Length:983
    Mass (Da):110,014
    Last modified:December 15, 2003 - v1
    Checksum:i7F4A0F7C6DDD130C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000045 Genomic DNA. Translation: BAC91592.1.
    RefSeqiNP_926597.1. NC_005125.1.
    WP_011143640.1. NC_005125.1.

    Genome annotation databases

    EnsemblBacteriaiBAC91592; BAC91592; BAC91592.
    GeneIDi2602055.
    KEGGigvi:gvip495.
    PATRICi22046801. VBIGloVio86258_3686.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000045 Genomic DNA. Translation: BAC91592.1 .
    RefSeqi NP_926597.1. NC_005125.1.
    WP_011143640.1. NC_005125.1.

    3D structure databases

    ProteinModelPortali Q7NF75.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 251221.gvip495.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC91592 ; BAC91592 ; BAC91592 .
    GeneIDi 2602055.
    KEGGi gvi:gvip495.
    PATRICi 22046801. VBIGloVio86258_3686.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.
    PhylomeDBi Q7NF75.

    Enzyme and pathway databases

    BioCyci GVIO251221:GH9A-3700-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7421.

    Entry informationi

    Entry nameiSYI_GLOVI
    AccessioniPrimary (citable) accession number: Q7NF75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3