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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei608Aminoacyl-adenylateUniRule annotation1
Binding sitei652ATPUniRule annotation1
Metal bindingi952ZincUniRule annotation1
Metal bindingi955ZincUniRule annotation1
Metal bindingi972ZincUniRule annotation1
Metal bindingi975ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciGVIO251221:G1G3K-3699-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:gll3651
OrganismiGloeobacter violaceus (strain PCC 7421)
Taxonomic identifieri251221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacteraceaeGloeobacter
Proteomesi
  • UP000000557 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983921 – 983Isoleucine--tRNA ligaseAdd BLAST983

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi251221.gvip495

Structurei

3D structure databases

ProteinModelPortaliQ7NF75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 71"HIGH" regionAdd BLAST11
Motifi649 – 653"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
InParanoidiQ7NF75
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I
PhylomeDBiQ7NF75

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 2 hits
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q7NF75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTPVDYKQ TVRTPKTDFP MRANASTREV EIQRFWEEQG IYGRLAQENP
60 70 80 90 100
GEIFILHDGP PYANGELHVG HALNKILKDI INRYQLLQGR RVRYVPGWDC
110 120 130 140 150
HGLPIELKVL QDLSQAERSQ LTPLDVRTRA RDFAQRTVQS QCASYKRFGV
160 170 180 190 200
WGDWDHPYLT LQPEYEAAQL GVFGKMALKG YIYRGLKPVH WSPSSQTALA
210 220 230 240 250
EAELEYPTKD DGSPAHTSRS VYVKFPLISI AAPETATVMA AELLPRLSAF
260 270 280 290 300
HDREEELIDA LLGERDDLPE IAVAIWTTTP WTLPGNLAVA LNGELDYALV
310 320 330 340 350
ASDEHGLLIV AAELVERLAG TLATRFETIA TFRGRELEGS LLAHPLFRRT
360 370 380 390 400
SPIVLGDHVT TESGTGAVHT APGHGSEDFE LAQRYNLGVL SPVDDYGRFT
410 420 430 440 450
READSDRREN LPVFAGKAVL SDGNQAVIEA LSAAGALLKE EAYVHKYPYD
460 470 480 490 500
WRTKKPTIFR ATTQWFASVS DFRPQALSAI AQTEWIPASG ENRITAMVAG
510 520 530 540 550
RNDWCISRQR AWGLPIPAFY CENCANVLLT QESVEAVQAA VRVHGSDIWW
560 570 580 590 600
QKEASELLPP GIACAHCGGT AFRKEKDIMD VWFDSGSSWA GVLGRRPELH
610 620 630 640 650
YPADVYLEGS DQHRGWFQSS LLTCVATEGT APYKTVITHG FTLDEHGRKM
660 670 680 690 700
SKSLGNVVDP KLVIDGGANQ KQHPAYGADV LRLWVASVDY TSDQLVGPSV
710 720 730 740 750
LAQIAEVYRK IRNTARYLLG SLNDFVPERD LVAFDSLGEV DQYLLHRLSV
760 770 780 790 800
VHLEVTQAFE SYQFSRFFQT IQNFCVADLS NFYLDISKDR LYISAEVSLR
810 820 830 840 850
RRSCQTVLYR VLESLTRLIA PVLPHLAEDI WQHLPYPRSD ASVFEAGWPV
860 870 880 890 900
DHSQWFQPLT VDRWPGLIVL RDRVNIALEA ARNAKRIGSS LEAKIRLHVE
910 920 930 940 950
DPALTDELAR QKDELRYLFI VSQVELLDEL PAEVSVEEGA AVIVLDADGQ
960 970 980
KCERCWNYSV HVGEDAEHPT LCERCVSALA GAF
Length:983
Mass (Da):110,014
Last modified:December 15, 2003 - v1
Checksum:i7F4A0F7C6DDD130C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA Translation: BAC91592.1
RefSeqiNP_926597.1, NC_005125.1
WP_011143640.1, NC_005125.1

Genome annotation databases

EnsemblBacteriaiBAC91592; BAC91592; BAC91592
GeneIDi2602055
KEGGigvi:gvip495
PATRICifig|251221.4.peg.3686

Similar proteinsi

Entry informationi

Entry nameiSYI_GLOVI
AccessioniPrimary (citable) accession number: Q7NF75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 15, 2003
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health