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Q7NF44 (GSHB_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:gll3682
OrganismGloeobacter violaceus (strain PCC 7421) [Reference proteome] [HAMAP]
Taxonomic identifier251221 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197468

Regions

Domain129 – 313185ATP-grasp
Nucleotide binding155 – 21157ATP By similarity

Sites

Metal binding2841Magnesium or manganese By similarity
Metal binding2861Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NF44 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 7D3277C55E838EAF

FASTA32435,906
        10         20         30         40         50         60 
MKILFIVDPL ETLKPGHDTS VALMQAAARR GHSVWAAEVG DLQVHHHRAA AQVRTLTIHP 

        70         80         90        100        110        120 
GRTPFYTVEA VGFYPLSEAD VIWMRKDPPV TSAYLWATQV LDLVNAGRGD GRTTFVLNRP 

       130        140        150        160        170        180 
SGLRNDNEKL YALHFPDLVP ETRVCTHRQD ILDFVDIHGR AVIKPLDGKG GEGIFLLARA 

       190        200        210        220        230        240 
DRNLNAIIEA STAYGTRHVM VQRYLEESRQ GDKRIVLLAG EPIGALLRVP REDDVRGNMA 

       250        260        270        280        290        300 
AGGRVVKTTL TERDREICRV VGPRLVADGH YFVGIDVIGA YLTEINVTSP TGICEIDVLD 

       310        320 
GVVLEDEIVD WLVEYTRPAL ARNL 

« Hide

References

[1]"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids."
Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S.
DNA Res. 10:137-145(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7421.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000045 Genomic DNA. Translation: BAC91623.1.
RefSeqNP_926628.1. NC_005125.1.

3D structure databases

ProteinModelPortalQ7NF44.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING251221.gvip498.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC91623; BAC91623; BAC91623.
GeneID2602086.
KEGGgvi:gvip498.
PATRIC22046861. VBIGloVio86258_3716.

Phylogenomic databases

eggNOGCOG0189.
KOK01920.
OMAMDAVFMR.
OrthoDBEOG6WMHWB.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycGVIO251221:GH9A-3731-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_GLOVI
AccessionPrimary (citable) accession number: Q7NF44
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways