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Protein

Glutathione synthetase

Gene

gshB

Organism
Gloeobacter violaceus (strain PCC 7421)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi284 – 2841Magnesium or manganeseUniRule annotation
Metal bindingi286 – 2861Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi155 – 21157ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGVIO251221:GH9A-3731-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:gll3682
OrganismiGloeobacter violaceus (strain PCC 7421)
Taxonomic identifieri251221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter
ProteomesiUP000000557 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Glutathione synthetasePRO_0000197468Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi251221.gvip498.

Structurei

3D structure databases

ProteinModelPortaliQ7NF44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 313185ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0189.
InParanoidiQ7NF44.
KOiK01920.
OMAiNEKMYAL.
OrthoDBiEOG6WMHWB.
PhylomeDBiQ7NF44.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NF44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILFIVDPL ETLKPGHDTS VALMQAAARR GHSVWAAEVG DLQVHHHRAA
60 70 80 90 100
AQVRTLTIHP GRTPFYTVEA VGFYPLSEAD VIWMRKDPPV TSAYLWATQV
110 120 130 140 150
LDLVNAGRGD GRTTFVLNRP SGLRNDNEKL YALHFPDLVP ETRVCTHRQD
160 170 180 190 200
ILDFVDIHGR AVIKPLDGKG GEGIFLLARA DRNLNAIIEA STAYGTRHVM
210 220 230 240 250
VQRYLEESRQ GDKRIVLLAG EPIGALLRVP REDDVRGNMA AGGRVVKTTL
260 270 280 290 300
TERDREICRV VGPRLVADGH YFVGIDVIGA YLTEINVTSP TGICEIDVLD
310 320
GVVLEDEIVD WLVEYTRPAL ARNL
Length:324
Mass (Da):35,906
Last modified:December 15, 2003 - v1
Checksum:i7D3277C55E838EAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC91623.1.
RefSeqiNP_926628.1. NC_005125.1.
WP_011143671.1. NC_005125.1.

Genome annotation databases

EnsemblBacteriaiBAC91623; BAC91623; BAC91623.
GeneIDi2602086.
KEGGigvi:gvip498.
PATRICi22046861. VBIGloVio86258_3716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000045 Genomic DNA. Translation: BAC91623.1.
RefSeqiNP_926628.1. NC_005125.1.
WP_011143671.1. NC_005125.1.

3D structure databases

ProteinModelPortaliQ7NF44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi251221.gvip498.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC91623; BAC91623; BAC91623.
GeneIDi2602086.
KEGGigvi:gvip498.
PATRICi22046861. VBIGloVio86258_3716.

Phylogenomic databases

eggNOGiCOG0189.
InParanoidiQ7NF44.
KOiK01920.
OMAiNEKMYAL.
OrthoDBiEOG6WMHWB.
PhylomeDBiQ7NF44.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
BioCyciGVIO251221:GH9A-3731-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7421.

Entry informationi

Entry nameiGSHB_GLOVI
AccessioniPrimary (citable) accession number: Q7NF44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: May 27, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.