Reviewed, UniProtKB/Swiss-Prot Q7NF44 (GSHB_GLOVI)

Names and origin

Protein names

Recommended name:
    Glutathione synthetase
    EC=6.3.2.3
Alternative name(s):
    Glutathione synthase
    GSH synthetase
      Short name=GSH-S
      Short name=GSHase

Gene names

Name:	gshB
Ordered Locus Names:	gll3682

Organism

Gloeobacter violaceus [Complete proteome] [HAMAP]

Taxonomic identifier

33072 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Glutathione synthetase HAMAP MF_00162		PRO_0000197468
Regions
	Domain	129 – 313	185	ATP-grasp
	Nucleotide binding	155 – 211	57	ATP By similarity
Sites
	Metal binding	284	1	Magnesium or manganese By similarity
	Metal binding	286	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NF44-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 7D3277C55E838EAF	FASTA	324	35,906
	10 20 30 40 50 60 MKILFIVDPL ETLKPGHDTS VALMQAAARR GHSVWAAEVG DLQVHHHRAA AQVRTLTIHP 70 80 90 100 110 120 GRTPFYTVEA VGFYPLSEAD VIWMRKDPPV TSAYLWATQV LDLVNAGRGD GRTTFVLNRP 130 140 150 160 170 180 SGLRNDNEKL YALHFPDLVP ETRVCTHRQD ILDFVDIHGR AVIKPLDGKG GEGIFLLARA 190 200 210 220 230 240 DRNLNAIIEA STAYGTRHVM VQRYLEESRQ GDKRIVLLAG EPIGALLRVP REDDVRGNMA 250 260 270 280 290 300 AGGRVVKTTL TERDREICRV VGPRLVADGH YFVGIDVIGA YLTEINVTSP TGICEIDVLD 310 320 GVVLEDEIVD WLVEYTRPAL ARNL

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000045 Genomic DNA. Translation: BAC91623.1.
RefSeq	NP_926628.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2602086.
GenomeReviews	Gene locus gll3682 in contig BA000045_GR.
NMPDR	fig|251221.1.peg.3682.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NF44.
OMA	Q7NF44. VRNAPEK.
Enzyme and pathway databases
BioCyc	GVIO251221:GLL3682-MON.
BRENDA	6.3.2.3. 276183.
Family and domain databases
HAMAP	MF_00162. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP_bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_GLOVI

Accession

Primary (citable) accession number: Q7NF44

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)