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Q7NE01 (SYL_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:gll4081
OrganismGloeobacter violaceus (strain PCC 7421) [Reference proteome] [HAMAP]
Taxonomic identifier251221 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter

Protein attributes

Sequence length847 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 847847Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152021

Regions

Motif39 – 4911"HIGH" region HAMAP-Rule MF_00049
Motif613 – 6175"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6161ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NE01 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 4C53540A83DA7029

FASTA84795,295
        10         20         30         40         50         60 
METRYNPHAI EPRRQKQWEE APHLAMDGRP KFYALSMFPY PSGALHMGHV RNYSITDVIS 

        70         80         90        100        110        120 
RYKRMRGFNV LHPIGWDAFG LPAENAAIDR GIHPAQWTEQ NIAQMREQLK RLGFAYAWER 

       130        140        150        160        170        180 
EVATCSPAYY RWTQKLFLEF WKAGLAYRKA GVVNWDPVDQ TVLANEQVDA EGRSWRSGAL 

       190        200        210        220        230        240 
VEKRPLEQWY LKITDYAEEL LQALGTLGDW PERVRVMQEN WIGKSVGAEL CFPINGEPEG 

       250        260        270        280        290        300 
IRVFTTRPDT VYGVTYLVLA PEHPLVERIT APERREAVRA FVAQVQSESE IERVSEDRPK 

       310        320        330        340        350        360 
QGVSTGAVAL NPFTGQAVPV WIADYVLFEY GTGAVMGVPG HDERDFVFAS QYELPIRLVV 

       370        380        390        400        410        420 
QAPDGSLTEP LRAAYTEVGV LVNSGPFNGL DSPTGKLKIV EYAEQQGWGK GRVQYRLRDW 

       430        440        450        460        470        480 
LISRQRYWGC PIPMVYCPEC GVVPVPDEQL PVALPGDVEF SGRGPSPLAK LEGWICVDCP 

       490        500        510        520        530        540 
QCGAPARRET DTMDTFIDSS WYFLRFADAR NGAEPFSREA VDYWLPVDQY VGGIEHAILH 

       550        560        570        580        590        600 
LLYSRFFTKV LRDRGLLSFD EPFKRLLTQG MVLSNAFVDP ATKKYYPPDQ VEERGGAFFA 

       610        620        630        640        650        660 
RPDGTPLVCA MEKMSKSKYN GIDPLTVRSE YGADTARLFV LFKAPPEKEL EWSDADVRGQ 

       670        680        690        700        710        720 
YSFLGRVWRT VYEFVSGEKP DRPVGEAQER DLRREVHRAI QQVGGDIEQY KFNTAIAALM 

       730        740        750        760        770        780 
KLNNAMADYP SGQSPAYKEG VYVIVKLLAP FAPHIGAELW QALGEAGDIH TSDWPALDES 

       790        800        810        820        830        840 
ALVEETIVLV IQVNGKKRDD IQVPAAASEG ELQELALASE AVRRHTDGKA IKKVIVVPGR 


LINLVVG 

« Hide

References

[1]"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids."
Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S.
DNA Res. 10:137-145(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7421.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000045 Genomic DNA. Translation: BAC92022.1.
RefSeqNP_927027.1. NC_005125.1.

3D structure databases

ProteinModelPortalQ7NE01.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING251221.gvip546.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC92022; BAC92022; BAC92022.
GeneID2602464.
KEGGgvi:gvip546.
PATRIC22047661. VBIGloVio86258_4113.

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycGVIO251221:GH9A-4132-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GLOVI
AccessionPrimary (citable) accession number: Q7NE01
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: December 15, 2003
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries