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Q7NCG8 (SYW_GLOVI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:glr3011
OrganismGloeobacter violaceus (strain PCC 7421) [Reference proteome] [HAMAP]
Taxonomic identifier251221 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaGloeobacteriaGloeobacteralesGloeobacter

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136633

Regions

Motif17 – 259"HIGH" region HAMAP-Rule MF_00140
Motif200 – 2045"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2031ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NCG8 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 38D40A24C8F91ABB

FASTA33637,795
        10         20         30         40         50         60 
MPDDSTAPKR ILSGAQPTGQ LHLGNYLGAV RNWVSEQRQY DSYFCVVDLH ALTVPQEAAE 

        70         80         90        100        110        120 
LRAATRRTAA LYLACGIDPE RSTVFVQSHV SAHTELTWLF NCLTPINWLE RMIQFKEKAI 

       130        140        150        160        170        180 
KLGEEVGIGL FDYPVLQAAD ILLYEPHLVP VGEDQRQHLE LTRDIARRFN DRYGESLRVP 

       190        200        210        220        230        240 
EMLIRKEGAR VMSLQDGTSK MSKSDPSDLS RLNLLDAPEK LRDKIKRAKS DAVMGLKFDP 

       250        260        270        280        290        300 
ARPECTNLLT IYQLLSGESP EAVEARFADA GFGRFKPILA DLVIEYLRPI RERYDAIAGE 

       310        320        330 
SGYLEGILRE GALRASKVAG LTLERIRDRM GLLPPF 

« Hide

References

[1]"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids."
Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S.
DNA Res. 10:137-145(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7421.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000045 Genomic DNA. Translation: BAC90952.1.
RefSeqNP_925957.1. NC_005125.1.

3D structure databases

ProteinModelPortalQ7NCG8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING251221.gvip414.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC90952; BAC90952; BAC90952.
GeneID2601412.
KEGGgvi:gvip414.
PATRIC22045489. VBIGloVio86258_3038.

Phylogenomic databases

eggNOGCOG0180.
KOK01867.
OMAIDPKKHI.
OrthoDBEOG686NJQ.
PhylomeDBQ7NCG8.

Enzyme and pathway databases

BioCycGVIO251221:GH9A-3047-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_GLOVI
AccessionPrimary (citable) accession number: Q7NCG8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries