Reviewed, UniProtKB/Swiss-Prot Q7NCE1 (UCRI_GLOVI)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
      Short name=ISP
      Short name=RISP

Gene names

Name:	petC
Ordered Locus Names:	glr3038

Organism

Gloeobacter violaceus [Complete proteome] [HAMAP]

Taxonomic identifier

33072 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Gloeobacteria › Gloeobacterales › Gloeobacter

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin. HAMAP MF_01335
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Cell inner membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein. HAMAP MF_01335
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	Cytochrome b6-f complex iron-sulfur subunit HAMAP MF_01335		PRO_0000127774
Regions
	Transmembrane	16 – 38	23	Potential
	Domain	74 – 163	90	Rieske
Sites
	Metal binding	109	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	111	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	127	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	130	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	114 ↔ 129		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NCE1-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 5E80B2B76A847107	FASTA	186	19,638
	10 20 30 40 50 60 MSESAAQEIP MSRRQLLSFV TGGAIAATTA ATLYPVVLYF LPPSTAGGGE GVAAKDKEGK 70 80 90 100 110 120 DISVSKLLAA ATPGEPVLTL GLDVNGGDAT YIVINDQKEI ANFGINAVCT HLGCVVPWDN 130 140 150 160 170 180 GAKQFKCPCH GSVYNADGGL ERGPAPQPLA LVKATVSDDK VLIAPWTEQD FRCTDLWCNK DPYWVK

References

[1]

"Complete genome structure of Gloeobacter violaceus PCC 7421, a cyanobacterium that lacks thylakoids." Nakamura Y., Kaneko T., Sato S., Mimuro M., Miyashita H., Tsuchiya T., Sasamoto S., Watanabe A., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Takeuchi C., Yamada M., Tabata S. DNA Res. 10:137-145(2003) [PubMed: 14621292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7421.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000045 Genomic DNA. Translation: BAC90979.1.
RefSeq	NP_925984.1.
3D structure databases
HSSP	HSSP built from PDB template 1G8K based on UniProtKB Q7SIF3.
ModBase	Search...
Genome annotation databases
GeneID	2601439.
GenomeReviews	Gene locus glr3038 in contig BA000045_GR.
NMPDR	fig|251221.1.peg.3038.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NCE1.
OMA	Q7NCE1. YPVANYF.
Enzyme and pathway databases
BioCyc	GVIO251221:GLR3038-MON.
BRENDA	1.10.99.1. 276183.
Family and domain databases
HAMAP	MF_01335. [Tree]
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_GLOVI

Accession

Primary (citable) accession number: Q7NCE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)