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Q7NBZ0

- MNMA_MYCGA

UniProt

Q7NBZ0 - MNMA_MYCGA

Protein

Trifunctional protein RibF/MnmA

Gene

ribF/mnmA

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Involved in FAD and FMN biosynthesis.By similarity
    Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.By similarity

    Catalytic activityi

    ATP + riboflavin = ADP + FMN.
    ATP + FMN = diphosphate + FAD.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei318 – 3181ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Active sitei394 – 3941NucleophileBy similarity
    Binding sitei420 – 4201ATP; via amide nitrogenBy similarity
    Sitei421 – 4211Interaction with tRNABy similarity
    Active sitei492 – 4921Cysteine persulfide intermediateBy similarity
    Sitei635 – 6351Interaction with tRNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi292 – 2998ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. FMN adenylyltransferase activity Source: UniProtKB-EC
    3. riboflavin kinase activity Source: UniProtKB-EC
    4. sulfurtransferase activity Source: InterPro
    5. tRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. FAD biosynthetic process Source: UniProtKB-UniPathway
    2. FMN biosynthetic process Source: UniProtKB-UniPathway
    3. riboflavin biosynthetic process Source: InterPro
    4. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciMGAL710127:GC09-126-MONOMER.
    UniPathwayiUPA00276; UER00406.
    UPA00277; UER00407.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trifunctional protein RibF/MnmA
    Including the following 3 domains:
    FMN adenylyltransferase (EC:2.7.7.2)
    Alternative name(s):
    FAD pyrophosphorylase
    FAD synthase
    Riboflavin kinase (EC:2.7.1.26)
    Alternative name(s):
    Flavokinase
    tRNA-specific 2-thiouridylase MnmA (EC:2.8.1.-)
    Gene namesi
    Name:ribF/mnmA
    Ordered Locus Names:MYCGA1200
    ORF Names:MGA_0832
    OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
    Taxonomic identifieri710127 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000001418: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Trifunctional protein RibF/MnmAPRO_0000349871Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi394 ↔ 492AlternateBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi233150.MGA_0832.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7NBZ0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 141141FMN adenylyltransferaseAdd
    BLAST
    Regioni158 – 282125Riboflavin kinaseAdd
    BLAST
    Regioni283 – 657375tRNA-specific 2-thiouridylase MnmAAdd
    BLAST
    Regioni389 – 3913Interaction with target base in tRNABy similarity
    Regioni442 – 4443Interaction with tRNABy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the RibF family.Curated
    In the C-terminal section; belongs to the MnmA/TRMU family.Curated

    Phylogenomic databases

    eggNOGiCOG0196.
    HOGENOMiHOG000113614.
    OrthoDBiEOG6RZB5H.

    Family and domain databases

    Gene3Di2.30.30.280. 1 hit.
    2.40.30.30. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
    InterProiIPR023382. Adenine_a_hdrlase_dom.
    IPR015864. FAD_synthase.
    IPR002606. Riboflavin_kinase_bac.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR004506. tRNA-specific_2-thiouridylase.
    [Graphical view]
    PANTHERiPTHR11933. PTHR11933. 1 hit.
    PfamiPF06574. FAD_syn. 1 hit.
    PF01687. Flavokinase. 1 hit.
    PF03054. tRNA_Me_trans. 1 hit.
    [Graphical view]
    SMARTiSM00904. Flavokinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF82114. SSF82114. 1 hit.
    TIGRFAMsiTIGR00083. ribF. 1 hit.
    TIGR00420. trmU. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7NBZ0-1 [UniParc]FASTAAdd to Basket

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    MLSIINLTSK TIKEVNKGVD LVIGFFDGIH KGHAKLFKQS DRFNLLTFDH    50
    IPKKQRLLYP KVDEIEQLSA LSGLEQLLVY DLLNNNLSAQ EFIDNYIKLI 100
    QPKRIIVGSD FKFGSDQVDY SLFAKNGYEV VVVKKDHCST SEIKKLIINC 150
    DLDQANKLLL TPFYLKGTVI KNAQRGRTIG FVTANIILDN QLIELTEGSY 200
    VCKVIVDNKT YQGICFIGKP KTFDEKQRQC EAHIFDFDQD IYGKKIKVEL 250
    YQFIRPTVKF NSINELKEAI ENDKKAALSF FHKQEKPKVV VALSGGVDSA 300
    VCAYLLQQQG YDVVAAFMQN WDKDLNFELL SDHADDQIQG CDAKQDYEDT 350
    QKLCEQLKIK LYHFNFVEQY WNDVFLKVLE DYKKGLTPNP DVLCNQFGKF 400
    GWFINALRKQ FGDDIKIAFG HYAKLITKDD EVFLVHTKDH NKDQTYFLTM 450
    LKKEQLKNII FPLSELDKPT VREIAKQANL YVANKKDSTG ICFIGERNFK 500
    QFLSNYLAIK KGPIILIDEN KKIGEHDGLY FYTIGQSRRL HVGGTKEKIF 550
    VCDKDYNNNT LYVCYESSKD QYLSSVSCEL EKFNWLIDTK DQLFNKKLWI 600
    RFRHRQKLQE CEIVSYHDDK VIVKYTKQIG VTPGQYGVIY DQNLWVVGGG 650
    KITKIIK 657
    Length:657
    Mass (Da):75,974
    Last modified:December 15, 2003 - v1
    Checksum:iC2F795910B8821EF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015450 Genomic DNA. Translation: AAP56470.1.
    RefSeqiNP_852902.1. NC_004829.2.
    WP_011113349.1. NC_004829.2.

    Genome annotation databases

    EnsemblBacteriaiAAP56470; AAP56470; MGA_0832.
    GeneIDi1090170.
    KEGGimga:MGA_0832.
    PATRICi20007937. VBIMycGal115357_0134.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015450 Genomic DNA. Translation: AAP56470.1 .
    RefSeqi NP_852902.1. NC_004829.2.
    WP_011113349.1. NC_004829.2.

    3D structure databases

    ProteinModelPortali Q7NBZ0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233150.MGA_0832.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP56470 ; AAP56470 ; MGA_0832 .
    GeneIDi 1090170.
    KEGGi mga:MGA_0832.
    PATRICi 20007937. VBIMycGal115357_0134.

    Phylogenomic databases

    eggNOGi COG0196.
    HOGENOMi HOG000113614.
    OrthoDBi EOG6RZB5H.

    Enzyme and pathway databases

    UniPathwayi UPA00276 ; UER00406 .
    UPA00277 ; UER00407 .
    BioCyci MGAL710127:GC09-126-MONOMER.

    Family and domain databases

    Gene3Di 2.30.30.280. 1 hit.
    2.40.30.30. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00144. tRNA_thiouridyl_MnmA.
    InterProi IPR023382. Adenine_a_hdrlase_dom.
    IPR015864. FAD_synthase.
    IPR002606. Riboflavin_kinase_bac.
    IPR015865. Riboflavin_kinase_bac/euk.
    IPR023465. Riboflavin_kinase_domain.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR004506. tRNA-specific_2-thiouridylase.
    [Graphical view ]
    PANTHERi PTHR11933. PTHR11933. 1 hit.
    Pfami PF06574. FAD_syn. 1 hit.
    PF01687. Flavokinase. 1 hit.
    PF03054. tRNA_Me_trans. 1 hit.
    [Graphical view ]
    SMARTi SM00904. Flavokinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF82114. SSF82114. 1 hit.
    TIGRFAMsi TIGR00083. ribF. 1 hit.
    TIGR00420. trmU. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
      Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
      Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: R(low / passage 15 / clone 2).

    Entry informationi

    Entry nameiMNMA_MYCGA
    AccessioniPrimary (citable) accession number: Q7NBZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3