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Protein

Trifunctional protein RibF/MnmA

Gene

ribF/mnmA

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Involved in FAD and FMN biosynthesis.By similarity
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.By similarity

Catalytic activityi

ATP + riboflavin = ADP + FMN.
ATP + FMN = diphosphate + FAD.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei318 – 3181ATP; via amide nitrogen and carbonyl oxygenBy similarity
Active sitei394 – 3941NucleophileBy similarity
Binding sitei420 – 4201ATP; via amide nitrogenBy similarity
Sitei421 – 4211Interaction with tRNABy similarity
Active sitei492 – 4921Cysteine persulfide intermediateBy similarity
Sitei635 – 6351Interaction with tRNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi292 – 2998ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. FMN adenylyltransferase activity Source: UniProtKB-EC
  3. riboflavin kinase activity Source: UniProtKB-EC
  4. sulfurtransferase activity Source: InterPro
  5. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. FAD biosynthetic process Source: UniProtKB-UniPathway
  2. FMN biosynthetic process Source: UniProtKB-UniPathway
  3. riboflavin biosynthetic process Source: InterPro
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciMGAL710127:GC09-126-MONOMER.
UniPathwayiUPA00276; UER00406.
UPA00277; UER00407.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional protein RibF/MnmA
Including the following 3 domains:
FMN adenylyltransferase (EC:2.7.7.2)
Alternative name(s):
FAD pyrophosphorylase
FAD synthase
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
Flavokinase
tRNA-specific 2-thiouridylase MnmA (EC:2.8.1.-)
Gene namesi
Name:ribF/mnmA
Ordered Locus Names:MYCGA1200
ORF Names:MGA_0832
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001418 Componenti: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Trifunctional protein RibF/MnmAPRO_0000349871Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi394 ↔ 492AlternateBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi233150.MGA_0832.

Structurei

3D structure databases

ProteinModelPortaliQ7NBZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 141141FMN adenylyltransferaseAdd
BLAST
Regioni158 – 282125Riboflavin kinaseAdd
BLAST
Regioni283 – 657375tRNA-specific 2-thiouridylase MnmAAdd
BLAST
Regioni389 – 3913Interaction with target base in tRNABy similarity
Regioni442 – 4443Interaction with tRNABy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the RibF family.Curated
In the C-terminal section; belongs to the MnmA/TRMU family.Curated

Phylogenomic databases

eggNOGiCOG0196.
HOGENOMiHOG000113614.
OMAiVSCELEN.
OrthoDBiEOG6RZB5H.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
2.40.30.30. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR015864. FAD_synthase.
IPR002606. Riboflavin_kinase_bac.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
PfamiPF06574. FAD_syn. 1 hit.
PF01687. Flavokinase. 1 hit.
PF03054. tRNA_Me_trans. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.
TIGRFAMsiTIGR00083. ribF. 1 hit.
TIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7NBZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSIINLTSK TIKEVNKGVD LVIGFFDGIH KGHAKLFKQS DRFNLLTFDH
60 70 80 90 100
IPKKQRLLYP KVDEIEQLSA LSGLEQLLVY DLLNNNLSAQ EFIDNYIKLI
110 120 130 140 150
QPKRIIVGSD FKFGSDQVDY SLFAKNGYEV VVVKKDHCST SEIKKLIINC
160 170 180 190 200
DLDQANKLLL TPFYLKGTVI KNAQRGRTIG FVTANIILDN QLIELTEGSY
210 220 230 240 250
VCKVIVDNKT YQGICFIGKP KTFDEKQRQC EAHIFDFDQD IYGKKIKVEL
260 270 280 290 300
YQFIRPTVKF NSINELKEAI ENDKKAALSF FHKQEKPKVV VALSGGVDSA
310 320 330 340 350
VCAYLLQQQG YDVVAAFMQN WDKDLNFELL SDHADDQIQG CDAKQDYEDT
360 370 380 390 400
QKLCEQLKIK LYHFNFVEQY WNDVFLKVLE DYKKGLTPNP DVLCNQFGKF
410 420 430 440 450
GWFINALRKQ FGDDIKIAFG HYAKLITKDD EVFLVHTKDH NKDQTYFLTM
460 470 480 490 500
LKKEQLKNII FPLSELDKPT VREIAKQANL YVANKKDSTG ICFIGERNFK
510 520 530 540 550
QFLSNYLAIK KGPIILIDEN KKIGEHDGLY FYTIGQSRRL HVGGTKEKIF
560 570 580 590 600
VCDKDYNNNT LYVCYESSKD QYLSSVSCEL EKFNWLIDTK DQLFNKKLWI
610 620 630 640 650
RFRHRQKLQE CEIVSYHDDK VIVKYTKQIG VTPGQYGVIY DQNLWVVGGG

KITKIIK
Length:657
Mass (Da):75,974
Last modified:December 15, 2003 - v1
Checksum:iC2F795910B8821EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015450 Genomic DNA. Translation: AAP56470.1.
RefSeqiNP_852902.1. NC_004829.2.
WP_011113349.1. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56470; AAP56470; MGA_0832.
GeneIDi1090170.
KEGGimga:MGA_0832.
PATRICi20007937. VBIMycGal115357_0134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015450 Genomic DNA. Translation: AAP56470.1.
RefSeqiNP_852902.1. NC_004829.2.
WP_011113349.1. NC_004829.2.

3D structure databases

ProteinModelPortaliQ7NBZ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233150.MGA_0832.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP56470; AAP56470; MGA_0832.
GeneIDi1090170.
KEGGimga:MGA_0832.
PATRICi20007937. VBIMycGal115357_0134.

Phylogenomic databases

eggNOGiCOG0196.
HOGENOMiHOG000113614.
OMAiVSCELEN.
OrthoDBiEOG6RZB5H.

Enzyme and pathway databases

UniPathwayiUPA00276; UER00406.
UPA00277; UER00407.
BioCyciMGAL710127:GC09-126-MONOMER.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
2.40.30.30. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR015864. FAD_synthase.
IPR002606. Riboflavin_kinase_bac.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023465. Riboflavin_kinase_domain.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
PfamiPF06574. FAD_syn. 1 hit.
PF01687. Flavokinase. 1 hit.
PF03054. tRNA_Me_trans. 1 hit.
[Graphical view]
SMARTiSM00904. Flavokinase. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.
TIGRFAMsiTIGR00083. ribF. 1 hit.
TIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
    Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
    Microbiology 149:2307-2316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R(low / passage 15 / clone 2).

Entry informationi

Entry nameiMNMA_MYCGA
AccessioniPrimary (citable) accession number: Q7NBZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: December 15, 2003
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.