Reviewed, UniProtKB/Swiss-Prot Q7NBZ0 (MNMA_MYCGA)

Names and origin

Protein names

Recommended name:
    Trifunctional protein ribF/mnmA
Including the following 3 domains:
    1- Recommended name:
            FMN adenylyltransferase
              EC=2.7.7.2
        Alternative name(s):
            FAD pyrophosphorylase
            FAD synthetase
    2- Recommended name:
            Riboflavin kinase
              EC=2.7.1.26
        Alternative name(s):
            Flavokinase
    3- Recommended name:
            tRNA-specific 2-thiouridylase mnmA
              EC=2.8.1.-

Gene names

Name:	ribF/mnmA
Ordered Locus Names:	MYCGA1200
ORF Names:	MGA_0832

Organism

Mycoplasma gallisepticum [Complete proteome] [HAMAP]

Taxonomic identifier

2096 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in FAD and FMN biosynthesis By similarity. Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34 By similarity.
Catalytic activity	ATP + riboflavin = ADP + FMN. HAMAP MF_00144 ATP + FMN = diphosphate + FAD. HAMAP MF_00144
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. HAMAP MF_00144 Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1. HAMAP MF_00144
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the ribF family. In the C-terminal section; belongs to the mnmA/TRMU family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding FAD FMN Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Nucleotidyltransferase Transferase
PTM	Disulfide bond
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	Trifunctional protein ribF/mnmA HAMAP MF_00144		PRO_0000349871
Regions
	Nucleotide binding	292 – 299	8	ATP By similarity
	Region	1 – 141	141	FMN adenylyltransferase HAMAP MF_00144
	Region	158 – 282	125	Riboflavin kinase HAMAP MF_00144
	Region	283 – 657	375	tRNA-specific 2-thiouridylase mnmA HAMAP MF_00144
	Region	389 – 391	3	Interaction with target base in tRNA By similarity
	Region	442 – 444	3	Interaction with tRNA By similarity
Sites
	Active site	394	1	Nucleophile By similarity
	Active site	492	1	Cysteine persulfide intermediate By similarity
	Binding site	318	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	420	1	ATP; via amide nitrogen By similarity
	Site	421	1	Interaction with tRNA By similarity
	Site	635	1	Interaction with tRNA By similarity
Amino acid modifications
	Disulfide bond	394 ↔ 492		Alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NBZ0-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: C2F795910B8821EF	FASTA	657	75,974
	10 20 30 40 50 60 MLSIINLTSK TIKEVNKGVD LVIGFFDGIH KGHAKLFKQS DRFNLLTFDH IPKKQRLLYP 70 80 90 100 110 120 KVDEIEQLSA LSGLEQLLVY DLLNNNLSAQ EFIDNYIKLI QPKRIIVGSD FKFGSDQVDY 130 140 150 160 170 180 SLFAKNGYEV VVVKKDHCST SEIKKLIINC DLDQANKLLL TPFYLKGTVI KNAQRGRTIG 190 200 210 220 230 240 FVTANIILDN QLIELTEGSY VCKVIVDNKT YQGICFIGKP KTFDEKQRQC EAHIFDFDQD 250 260 270 280 290 300 IYGKKIKVEL YQFIRPTVKF NSINELKEAI ENDKKAALSF FHKQEKPKVV VALSGGVDSA 310 320 330 340 350 360 VCAYLLQQQG YDVVAAFMQN WDKDLNFELL SDHADDQIQG CDAKQDYEDT QKLCEQLKIK 370 380 390 400 410 420 LYHFNFVEQY WNDVFLKVLE DYKKGLTPNP DVLCNQFGKF GWFINALRKQ FGDDIKIAFG 430 440 450 460 470 480 HYAKLITKDD EVFLVHTKDH NKDQTYFLTM LKKEQLKNII FPLSELDKPT VREIAKQANL 490 500 510 520 530 540 YVANKKDSTG ICFIGERNFK QFLSNYLAIK KGPIILIDEN KKIGEHDGLY FYTIGQSRRL 550 560 570 580 590 600 HVGGTKEKIF VCDKDYNNNT LYVCYESSKD QYLSSVSCEL EKFNWLIDTK DQLFNKKLWI 610 620 630 640 650 RFRHRQKLQE CEIVSYHDDK VIVKYTKQIG VTPGQYGVIY DQNLWVVGGG KITKIIK

References

[1]

"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)." Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J. Microbiology 149:2307-2316(2003) [PubMed: 12949158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R(low).

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015450 Genomic DNA. Translation: AAP56470.1.
RefSeq	NP_852902.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1090170.
GenomeReviews	Gene locus MYCGA1200 in contig AE015450_GR.
KEGG	mga:MGA_0832.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NBZ0.
Enzyme and pathway databases
BioCyc	MGAL233150:MGA_0832-MONOMER.
Family and domain databases
HAMAP	MF_00144. Fused. [Tree]
InterPro	IPR015864. FAD_synthase. IPR015865. Riboflavin_kinase. IPR002606. Riboflavin_kinase/FAD_synth. IPR004506. TrmU_MeTrfase. IPR018318. tRNA_MeTrfase-like. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit.
PANTHER	PTHR11933. TrmU_mtfrase. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. PF01687. Flavokinase. 1 hit. PF03054. tRNA_Me_trans. 1 hit. [Graphical view]
ProDom	PD003662. FAD_Synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00083. ribF. 1 hit. TIGR00420. trmU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNMA_MYCGA

Accession

Primary (citable) accession number: Q7NBZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)