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Q7NBG2 (TRMB_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MYCGA3170
ORF Names:MGA_1194
OrganismMycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]
Taxonomic identifier710127 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171350

Regions

Region192 – 1954Substrate binding By similarity

Sites

Active site1131 By similarity
Binding site381S-adenosyl-L-methionine By similarity
Binding site631S-adenosyl-L-methionine By similarity
Binding site911S-adenosyl-L-methionine By similarity
Binding site1131S-adenosyl-L-methionine By similarity
Binding site1171Substrate By similarity
Binding site1491Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NBG2 [UniParc].

Last modified June 15, 2010. Version 2.
Checksum: 8D1439AC1CF0349D

FASTA21324,748
        10         20         30         40         50         60 
MRIRNIRDAH LKINKAKNII GVDNLKTKLD PKKFNALEIG SGKGGFIYQK AITNPGINYF 

        70         80         90        100        110        120 
GIEKNATVIL KMINKSELLE QLTNLFIVHD DFALIDHEFP NACFDQIYLN FSDPWPKKRH 

       130        140        150        160        170        180 
AKKRLVDLAF LNKYQRILKA DGIIEFKTDN DQLFNYAIEM INSLEQIKII AYTNDLHSLD 

       190        200        210 
SSDALLKNNV ITEYEQRFIN LKKNINKIVF KFI 

« Hide

References

[1]"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015450 Genomic DNA. Translation: AAP56667.2.
RefSeqNP_853099.2. NC_004829.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233150.MGA_1194.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP56667; AAP56667; MGA_1194.
GeneID1089649.
KEGGmga:MGA_1194.
PATRIC20008431. VBIMycGal115357_0351.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.

Enzyme and pathway databases

BioCycMGAL710127:GC09-372-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCGA
AccessionPrimary (citable) accession number: Q7NBG2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 15, 2010
Last modified: June 11, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways