Q7NBG0 (SYI_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	MYCGA3190
ORF Names:	MGA_1196

Organism

Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]

Taxonomic identifier

710127 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma gallisepticum › Mycoplasma gallisepticum str. R

Protein attributes

Sequence length	897 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 897	897	Isoleucine--tRNA ligase HAMAP-Rule MF_02002		PRO_0000098416
Regions
	Motif	59 – 69	11	"HIGH" region HAMAP-Rule MF_02002
	Motif	593 – 597	5	"KMSKS" region HAMAP-Rule MF_02002
Sites
	Metal binding	872	1	Zinc By similarity
	Metal binding	875	1	Zinc By similarity
	Metal binding	890	1	Zinc By similarity
	Metal binding	893	1	Zinc By similarity
	Binding site	552	1	Aminoacyl-adenylate By similarity
	Binding site	596	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NBG0 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: 74C2C97A90FB69E8	FASTA	897	104,555
	10 20 30 40 50 60 MNKNYKDTLL MPSTDFEMKA NLATKESKIQ QKWLDDQIYQ LRLEKNQNNE QKILHDGPPY 70 80 90 100 110 120 ANGDIHVGHT MNKILKDVIV RRWLMQGYYS PFILGWDTHG LPIEHAVQSK LGQKEFFKLS 130 140 150 160 170 180 VLERIEVCRD FAFNQVQKQK EQFSSLGLVT DFKVCYHTYD KQYEIDQLKV FAKMINEGLV 190 200 210 220 230 240 YQDYKPIYWS WSSKSVLSDA EIEYKETKSP SIYVTFKTLD NEVIGKDVNL VIWTTTPWTL 250 260 270 280 290 300 PSNLAISVHP ELEYTLFEVN GQKYLVGSNL YEQLIAKFNF ENPKVIQKLK GSSLDKIKYQ 310 320 330 340 350 360 HCLYDQITGI VVLGEHVSDS DGTGLVHTAP GFGLEDFIVC KKYGIDAYVP INDEGCFDQT 370 380 390 400 410 420 VHDPELVGVF YDDANKLIAQ KLEARNCLLS LNFINHQAAH DWRTKKPVIY RATKQWFINI 430 440 450 460 470 480 KSIKNQLVEN INTVKYPNER YAKRMLSMVA ERSDWCISRQ RTWGLPIPII FDENHEPILD 490 500 510 520 530 540 KELIDNTLRI MEAEGIKAWY EHDAKYFLTN KYDPRKTYYK ESDILDVWFD SGTSFNVLKE 550 560 570 580 590 600 NGIKDKATIY FEGSDQYRGW FNSSMICATV LNKTAPYKEL ISHGFTLDEK GMKMSKSQGN 610 620 630 640 650 660 VISPLTIIKN KGADILRLWA ASIDYSNDHR IGNHIIEQNS EIYRKIRNSL FRYILGNLND 670 680 690 700 710 720 FDFKPLDQYQ LSLADLLTIN HVNKSFKKID QAYLNYDYLE ITKEVNQMVV ELSAWYFELI 730 740 750 760 770 780 KDSLYCNDEN DPTRKAIQAT LNYIFINSLF RIAPILVHTA EEAYSHYQAT NKEKSVYLID 790 800 810 820 830 840 PPALFEIKTD LDLDQLATEF SKLKDDVYAE IEKLRKDKVL AKSNEAVVYL SKQYLDINKH 850 860 870 880 890 LVKQLKTWLN VAEVHFTKND QITVEKTDFS KCLRCWNYFK ELSKNNNEVC QRCSELV

References

[1]

"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)." Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J. Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015450 Genomic DNA. Translation: AAP56669.2.
RefSeq	NP_853101.2. NC_004829.2.
3D structure databases
ProteinModelPortal	Q7NBG0.
ModBase	Search...
Protein-protein interaction databases
STRING	233150.MGA_1196.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP56669; AAP56669; MGA_1196.
GeneID	1089653.
KEGG	mga:MGA_1196.
PATRIC	20008435. VBIMycGal115357_0353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246402.
KO	K01870.
ProtClustDB	PRK05743.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02002. Ile_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR010663. Znf_DNA_glyclase/IsotRNA_synth. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_MYCGA

Accession

Primary (citable) accession number: Q7NBG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program