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Q7NBG0

- SYI_MYCGA

UniProt

Q7NBG0 - SYI_MYCGA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi872 – 8721ZincUniRule annotation
    Metal bindingi875 – 8751ZincUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi893 – 8931ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMGAL710127:GC09-374-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MYCGA3190
    ORF Names:MGA_1196
    OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
    Taxonomic identifieri710127 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000001418: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 897897Isoleucine--tRNA ligasePRO_0000098416Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi233150.MGA_1196.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7NBG0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7NBG0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKNYKDTLL MPSTDFEMKA NLATKESKIQ QKWLDDQIYQ LRLEKNQNNE    50
    QKILHDGPPY ANGDIHVGHT MNKILKDVIV RRWLMQGYYS PFILGWDTHG 100
    LPIEHAVQSK LGQKEFFKLS VLERIEVCRD FAFNQVQKQK EQFSSLGLVT 150
    DFKVCYHTYD KQYEIDQLKV FAKMINEGLV YQDYKPIYWS WSSKSVLSDA 200
    EIEYKETKSP SIYVTFKTLD NEVIGKDVNL VIWTTTPWTL PSNLAISVHP 250
    ELEYTLFEVN GQKYLVGSNL YEQLIAKFNF ENPKVIQKLK GSSLDKIKYQ 300
    HCLYDQITGI VVLGEHVSDS DGTGLVHTAP GFGLEDFIVC KKYGIDAYVP 350
    INDEGCFDQT VHDPELVGVF YDDANKLIAQ KLEARNCLLS LNFINHQAAH 400
    DWRTKKPVIY RATKQWFINI KSIKNQLVEN INTVKYPNER YAKRMLSMVA 450
    ERSDWCISRQ RTWGLPIPII FDENHEPILD KELIDNTLRI MEAEGIKAWY 500
    EHDAKYFLTN KYDPRKTYYK ESDILDVWFD SGTSFNVLKE NGIKDKATIY 550
    FEGSDQYRGW FNSSMICATV LNKTAPYKEL ISHGFTLDEK GMKMSKSQGN 600
    VISPLTIIKN KGADILRLWA ASIDYSNDHR IGNHIIEQNS EIYRKIRNSL 650
    FRYILGNLND FDFKPLDQYQ LSLADLLTIN HVNKSFKKID QAYLNYDYLE 700
    ITKEVNQMVV ELSAWYFELI KDSLYCNDEN DPTRKAIQAT LNYIFINSLF 750
    RIAPILVHTA EEAYSHYQAT NKEKSVYLID PPALFEIKTD LDLDQLATEF 800
    SKLKDDVYAE IEKLRKDKVL AKSNEAVVYL SKQYLDINKH LVKQLKTWLN 850
    VAEVHFTKND QITVEKTDFS KCLRCWNYFK ELSKNNNEVC QRCSELV 897
    Length:897
    Mass (Da):104,555
    Last modified:June 15, 2010 - v2
    Checksum:i74C2C97A90FB69E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015450 Genomic DNA. Translation: AAP56669.2.
    RefSeqiNP_853101.2. NC_004829.2.

    Genome annotation databases

    EnsemblBacteriaiAAP56669; AAP56669; MGA_1196.
    GeneIDi1089653.
    KEGGimga:MGA_1196.
    PATRICi20008435. VBIMycGal115357_0353.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015450 Genomic DNA. Translation: AAP56669.2 .
    RefSeqi NP_853101.2. NC_004829.2.

    3D structure databases

    ProteinModelPortali Q7NBG0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233150.MGA_1196.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP56669 ; AAP56669 ; MGA_1196 .
    GeneIDi 1089653.
    KEGGi mga:MGA_1196.
    PATRICi 20008435. VBIMycGal115357_0353.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.

    Enzyme and pathway databases

    BioCyci MGAL710127:GC09-374-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
      Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
      Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: R(low / passage 15 / clone 2).

    Entry informationi

    Entry nameiSYI_MYCGA
    AccessioniPrimary (citable) accession number: Q7NBG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3