Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q7NBG0

- SYI_MYCGA

UniProt

Q7NBG0 - SYI_MYCGA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi872 – 8721ZincUniRule annotation
Metal bindingi875 – 8751ZincUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMGAL710127:GC09-374-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MYCGA3190
ORF Names:MGA_1196
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001418: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 897897Isoleucine--tRNA ligasePRO_0000098416Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi233150.MGA_1196.

Structurei

3D structure databases

ProteinModelPortaliQ7NBG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NBG0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKNYKDTLL MPSTDFEMKA NLATKESKIQ QKWLDDQIYQ LRLEKNQNNE
60 70 80 90 100
QKILHDGPPY ANGDIHVGHT MNKILKDVIV RRWLMQGYYS PFILGWDTHG
110 120 130 140 150
LPIEHAVQSK LGQKEFFKLS VLERIEVCRD FAFNQVQKQK EQFSSLGLVT
160 170 180 190 200
DFKVCYHTYD KQYEIDQLKV FAKMINEGLV YQDYKPIYWS WSSKSVLSDA
210 220 230 240 250
EIEYKETKSP SIYVTFKTLD NEVIGKDVNL VIWTTTPWTL PSNLAISVHP
260 270 280 290 300
ELEYTLFEVN GQKYLVGSNL YEQLIAKFNF ENPKVIQKLK GSSLDKIKYQ
310 320 330 340 350
HCLYDQITGI VVLGEHVSDS DGTGLVHTAP GFGLEDFIVC KKYGIDAYVP
360 370 380 390 400
INDEGCFDQT VHDPELVGVF YDDANKLIAQ KLEARNCLLS LNFINHQAAH
410 420 430 440 450
DWRTKKPVIY RATKQWFINI KSIKNQLVEN INTVKYPNER YAKRMLSMVA
460 470 480 490 500
ERSDWCISRQ RTWGLPIPII FDENHEPILD KELIDNTLRI MEAEGIKAWY
510 520 530 540 550
EHDAKYFLTN KYDPRKTYYK ESDILDVWFD SGTSFNVLKE NGIKDKATIY
560 570 580 590 600
FEGSDQYRGW FNSSMICATV LNKTAPYKEL ISHGFTLDEK GMKMSKSQGN
610 620 630 640 650
VISPLTIIKN KGADILRLWA ASIDYSNDHR IGNHIIEQNS EIYRKIRNSL
660 670 680 690 700
FRYILGNLND FDFKPLDQYQ LSLADLLTIN HVNKSFKKID QAYLNYDYLE
710 720 730 740 750
ITKEVNQMVV ELSAWYFELI KDSLYCNDEN DPTRKAIQAT LNYIFINSLF
760 770 780 790 800
RIAPILVHTA EEAYSHYQAT NKEKSVYLID PPALFEIKTD LDLDQLATEF
810 820 830 840 850
SKLKDDVYAE IEKLRKDKVL AKSNEAVVYL SKQYLDINKH LVKQLKTWLN
860 870 880 890
VAEVHFTKND QITVEKTDFS KCLRCWNYFK ELSKNNNEVC QRCSELV
Length:897
Mass (Da):104,555
Last modified:June 15, 2010 - v2
Checksum:i74C2C97A90FB69E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015450 Genomic DNA. Translation: AAP56669.2.
RefSeqiNP_853101.2. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56669; AAP56669; MGA_1196.
GeneIDi1089653.
KEGGimga:MGA_1196.
PATRICi20008435. VBIMycGal115357_0353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015450 Genomic DNA. Translation: AAP56669.2 .
RefSeqi NP_853101.2. NC_004829.2.

3D structure databases

ProteinModelPortali Q7NBG0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233150.MGA_1196.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP56669 ; AAP56669 ; MGA_1196 .
GeneIDi 1089653.
KEGGi mga:MGA_1196.
PATRICi 20008435. VBIMycGal115357_0353.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.

Enzyme and pathway databases

BioCyci MGAL710127:GC09-374-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
    Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
    Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R(low / passage 15 / clone 2).

Entry informationi

Entry nameiSYI_MYCGA
AccessioniPrimary (citable) accession number: Q7NBG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 15, 2010
Last modified: November 26, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3