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Q7NBG0

- SYI_MYCGA

UniProt

Q7NBG0 - SYI_MYCGA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, MYCGA3190, MGA_1196
Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylate By similarity
Binding sitei596 – 5961ATP By similarity
Metal bindingi872 – 8721Zinc By similarity
Metal bindingi875 – 8751Zinc By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi893 – 8931Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMGAL710127:GC09-374-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MYCGA3190
ORF Names:MGA_1196
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001418: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 897897Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098416Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi233150.MGA_1196.

Structurei

3D structure databases

ProteinModelPortaliQ7NBG0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotation
Add
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NBG0-1 [UniParc]FASTAAdd to Basket

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MNKNYKDTLL MPSTDFEMKA NLATKESKIQ QKWLDDQIYQ LRLEKNQNNE    50
QKILHDGPPY ANGDIHVGHT MNKILKDVIV RRWLMQGYYS PFILGWDTHG 100
LPIEHAVQSK LGQKEFFKLS VLERIEVCRD FAFNQVQKQK EQFSSLGLVT 150
DFKVCYHTYD KQYEIDQLKV FAKMINEGLV YQDYKPIYWS WSSKSVLSDA 200
EIEYKETKSP SIYVTFKTLD NEVIGKDVNL VIWTTTPWTL PSNLAISVHP 250
ELEYTLFEVN GQKYLVGSNL YEQLIAKFNF ENPKVIQKLK GSSLDKIKYQ 300
HCLYDQITGI VVLGEHVSDS DGTGLVHTAP GFGLEDFIVC KKYGIDAYVP 350
INDEGCFDQT VHDPELVGVF YDDANKLIAQ KLEARNCLLS LNFINHQAAH 400
DWRTKKPVIY RATKQWFINI KSIKNQLVEN INTVKYPNER YAKRMLSMVA 450
ERSDWCISRQ RTWGLPIPII FDENHEPILD KELIDNTLRI MEAEGIKAWY 500
EHDAKYFLTN KYDPRKTYYK ESDILDVWFD SGTSFNVLKE NGIKDKATIY 550
FEGSDQYRGW FNSSMICATV LNKTAPYKEL ISHGFTLDEK GMKMSKSQGN 600
VISPLTIIKN KGADILRLWA ASIDYSNDHR IGNHIIEQNS EIYRKIRNSL 650
FRYILGNLND FDFKPLDQYQ LSLADLLTIN HVNKSFKKID QAYLNYDYLE 700
ITKEVNQMVV ELSAWYFELI KDSLYCNDEN DPTRKAIQAT LNYIFINSLF 750
RIAPILVHTA EEAYSHYQAT NKEKSVYLID PPALFEIKTD LDLDQLATEF 800
SKLKDDVYAE IEKLRKDKVL AKSNEAVVYL SKQYLDINKH LVKQLKTWLN 850
VAEVHFTKND QITVEKTDFS KCLRCWNYFK ELSKNNNEVC QRCSELV 897
Length:897
Mass (Da):104,555
Last modified:June 15, 2010 - v2
Checksum:i74C2C97A90FB69E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015450 Genomic DNA. Translation: AAP56669.2.
RefSeqiNP_853101.2. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56669; AAP56669; MGA_1196.
GeneIDi1089653.
KEGGimga:MGA_1196.
PATRICi20008435. VBIMycGal115357_0353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015450 Genomic DNA. Translation: AAP56669.2 .
RefSeqi NP_853101.2. NC_004829.2.

3D structure databases

ProteinModelPortali Q7NBG0.
ModBasei Search...

Protein-protein interaction databases

STRINGi 233150.MGA_1196.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP56669 ; AAP56669 ; MGA_1196 .
GeneIDi 1089653.
KEGGi mga:MGA_1196.
PATRICi 20008435. VBIMycGal115357_0353.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.

Enzyme and pathway databases

BioCyci MGAL710127:GC09-374-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
    Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
    Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R(low / passage 15 / clone 2).

Entry informationi

Entry nameiSYI_MYCGA
AccessioniPrimary (citable) accession number: Q7NBG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 15, 2010
Last modified: May 14, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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