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Q7NBG0 (SYI_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:MYCGA3190
ORF Names:MGA_1196
OrganismMycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]
Taxonomic identifier710127 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length897 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 897897Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098416

Regions

Motif59 – 6911"HIGH" region HAMAP-Rule MF_02002
Motif593 – 5975"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8721Zinc By similarity
Metal binding8751Zinc By similarity
Metal binding8901Zinc By similarity
Metal binding8931Zinc By similarity
Binding site5521Aminoacyl-adenylate By similarity
Binding site5961ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NBG0 [UniParc].

Last modified June 15, 2010. Version 2.
Checksum: 74C2C97A90FB69E8

FASTA897104,555
        10         20         30         40         50         60 
MNKNYKDTLL MPSTDFEMKA NLATKESKIQ QKWLDDQIYQ LRLEKNQNNE QKILHDGPPY 

        70         80         90        100        110        120 
ANGDIHVGHT MNKILKDVIV RRWLMQGYYS PFILGWDTHG LPIEHAVQSK LGQKEFFKLS 

       130        140        150        160        170        180 
VLERIEVCRD FAFNQVQKQK EQFSSLGLVT DFKVCYHTYD KQYEIDQLKV FAKMINEGLV 

       190        200        210        220        230        240 
YQDYKPIYWS WSSKSVLSDA EIEYKETKSP SIYVTFKTLD NEVIGKDVNL VIWTTTPWTL 

       250        260        270        280        290        300 
PSNLAISVHP ELEYTLFEVN GQKYLVGSNL YEQLIAKFNF ENPKVIQKLK GSSLDKIKYQ 

       310        320        330        340        350        360 
HCLYDQITGI VVLGEHVSDS DGTGLVHTAP GFGLEDFIVC KKYGIDAYVP INDEGCFDQT 

       370        380        390        400        410        420 
VHDPELVGVF YDDANKLIAQ KLEARNCLLS LNFINHQAAH DWRTKKPVIY RATKQWFINI 

       430        440        450        460        470        480 
KSIKNQLVEN INTVKYPNER YAKRMLSMVA ERSDWCISRQ RTWGLPIPII FDENHEPILD 

       490        500        510        520        530        540 
KELIDNTLRI MEAEGIKAWY EHDAKYFLTN KYDPRKTYYK ESDILDVWFD SGTSFNVLKE 

       550        560        570        580        590        600 
NGIKDKATIY FEGSDQYRGW FNSSMICATV LNKTAPYKEL ISHGFTLDEK GMKMSKSQGN 

       610        620        630        640        650        660 
VISPLTIIKN KGADILRLWA ASIDYSNDHR IGNHIIEQNS EIYRKIRNSL FRYILGNLND 

       670        680        690        700        710        720 
FDFKPLDQYQ LSLADLLTIN HVNKSFKKID QAYLNYDYLE ITKEVNQMVV ELSAWYFELI 

       730        740        750        760        770        780 
KDSLYCNDEN DPTRKAIQAT LNYIFINSLF RIAPILVHTA EEAYSHYQAT NKEKSVYLID 

       790        800        810        820        830        840 
PPALFEIKTD LDLDQLATEF SKLKDDVYAE IEKLRKDKVL AKSNEAVVYL SKQYLDINKH 

       850        860        870        880        890 
LVKQLKTWLN VAEVHFTKND QITVEKTDFS KCLRCWNYFK ELSKNNNEVC QRCSELV 

« Hide

References

[1]"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015450 Genomic DNA. Translation: AAP56669.2.
RefSeqNP_853101.2. NC_004829.2.

3D structure databases

ProteinModelPortalQ7NBG0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233150.MGA_1196.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP56669; AAP56669; MGA_1196.
GeneID1089653.
KEGGmga:MGA_1196.
PATRIC20008435. VBIMycGal115357_0353.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycMGAL710127:GC09-374-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_MYCGA
AccessionPrimary (citable) accession number: Q7NBG0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 15, 2010
Last modified: April 16, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries