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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei552Aminoacyl-adenylateUniRule annotation1
Binding sitei596ATPUniRule annotation1
Metal bindingi872ZincUniRule annotation1
Metal bindingi875ZincUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi893ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMGAL710127:GC09-368-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MYCGA3190
ORF Names:MGA_1196
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000001418 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984161 – 897Isoleucine--tRNA ligaseAdd BLAST897

Proteomic databases

PRIDEiQ7NBG0.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ7NBG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi593 – 597"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NBG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKNYKDTLL MPSTDFEMKA NLATKESKIQ QKWLDDQIYQ LRLEKNQNNE
60 70 80 90 100
QKILHDGPPY ANGDIHVGHT MNKILKDVIV RRWLMQGYYS PFILGWDTHG
110 120 130 140 150
LPIEHAVQSK LGQKEFFKLS VLERIEVCRD FAFNQVQKQK EQFSSLGLVT
160 170 180 190 200
DFKVCYHTYD KQYEIDQLKV FAKMINEGLV YQDYKPIYWS WSSKSVLSDA
210 220 230 240 250
EIEYKETKSP SIYVTFKTLD NEVIGKDVNL VIWTTTPWTL PSNLAISVHP
260 270 280 290 300
ELEYTLFEVN GQKYLVGSNL YEQLIAKFNF ENPKVIQKLK GSSLDKIKYQ
310 320 330 340 350
HCLYDQITGI VVLGEHVSDS DGTGLVHTAP GFGLEDFIVC KKYGIDAYVP
360 370 380 390 400
INDEGCFDQT VHDPELVGVF YDDANKLIAQ KLEARNCLLS LNFINHQAAH
410 420 430 440 450
DWRTKKPVIY RATKQWFINI KSIKNQLVEN INTVKYPNER YAKRMLSMVA
460 470 480 490 500
ERSDWCISRQ RTWGLPIPII FDENHEPILD KELIDNTLRI MEAEGIKAWY
510 520 530 540 550
EHDAKYFLTN KYDPRKTYYK ESDILDVWFD SGTSFNVLKE NGIKDKATIY
560 570 580 590 600
FEGSDQYRGW FNSSMICATV LNKTAPYKEL ISHGFTLDEK GMKMSKSQGN
610 620 630 640 650
VISPLTIIKN KGADILRLWA ASIDYSNDHR IGNHIIEQNS EIYRKIRNSL
660 670 680 690 700
FRYILGNLND FDFKPLDQYQ LSLADLLTIN HVNKSFKKID QAYLNYDYLE
710 720 730 740 750
ITKEVNQMVV ELSAWYFELI KDSLYCNDEN DPTRKAIQAT LNYIFINSLF
760 770 780 790 800
RIAPILVHTA EEAYSHYQAT NKEKSVYLID PPALFEIKTD LDLDQLATEF
810 820 830 840 850
SKLKDDVYAE IEKLRKDKVL AKSNEAVVYL SKQYLDINKH LVKQLKTWLN
860 870 880 890
VAEVHFTKND QITVEKTDFS KCLRCWNYFK ELSKNNNEVC QRCSELV
Length:897
Mass (Da):104,555
Last modified:June 15, 2010 - v2
Checksum:i74C2C97A90FB69E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015450 Genomic DNA. Translation: AAP56669.2.
RefSeqiWP_011113560.1. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56669; AAP56669; MGA_1196.
GeneIDi1089653.
KEGGimga:MGA_1196.
PATRICi20008435. VBIMycGal115357_0353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015450 Genomic DNA. Translation: AAP56669.2.
RefSeqiWP_011113560.1. NC_004829.2.

3D structure databases

ProteinModelPortaliQ7NBG0.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ7NBG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP56669; AAP56669; MGA_1196.
GeneIDi1089653.
KEGGimga:MGA_1196.
PATRICi20008435. VBIMycGal115357_0353.

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.

Enzyme and pathway databases

BioCyciMGAL710127:GC09-368-MONOMER.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_MYCGA
AccessioniPrimary (citable) accession number: Q7NBG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 15, 2010
Last modified: November 2, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.