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Q7NB47 (SYL_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MYCGA4330
ORF Names:MGA_0087
OrganismMycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]
Taxonomic identifier710127 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152044

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif583 – 5875"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5861ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NB47 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: A959DB38B8888710

FASTA80594,221
        10         20         30         40         50         60 
MYNHNLIEKK WAKIWNDQKI YSFQIDKNKP KYYILDMFPY PSGKGLHVGH VKAYMATDVI 

        70         80         90        100        110        120 
SRWKNALGFN VLHPIGWDAF GLPAEQYAIQ TNNHPAKFTQ ENINNFRTQL KRLGFNYDYR 

       130        140        150        160        170        180 
LEVDTTNKNY FKWTQWIFKK LYEHDLAYQA DIEVNWCEQL GTVLANEEVL TDENGNKISE 

       190        200        210        220        230        240 
RGSYPVIKKK MRQWVLKITA FADQLIDDLE NLNWPNSIKA MQVNWINKSV GASIKFEIDQ 

       250        260        270        280        290        300 
LDNQTIEVFS SRADTLFGAS FLALSFDHPL VKQKLITDKN NAIEQFIKDN SIDQRVRYQG 

       310        320        330        340        350        360 
INTNYFAIHP ITKKKIPIYL ADYILSDYGT GAVMGVPAHD ERDYQFAKQY DLEIIPVIKA 

       370        380        390        400        410        420 
DQYPYLLDGE HINSEFNNGL NNEQAIQKTI AYLREHNLGD QKINYKLRDW IFSRQRYWGE 

       430        440        450        460        470        480 
PFPVLFDEED NIYLLKDSEL PVELPQLSDF SPNKDGLPPL ANADDQWLHP IIDQKKYRRE 

       490        500        510        520        530        540 
INTMPQWAGS CWYYLAYLLK LTDLNQADGD QNYLALNSEK AKELFDHFMP VDLYVGGQEH 

       550        560        570        580        590        600 
AVLHLLYARF WYKFLHHIKI VSSTEPFSQL INQGMILGED NTKMSKSKGN IINPDDLVLS 

       610        620        630        640        650        660 
HGADTIRTYV MFMGPLNASL AWNSNALNGT RKFLERVYNL FDRVEINDSI NQNLNYDYHN 

       670        680        690        700        710        720 
FLKKINKHLE NFEFNLVVSE MMIFINACYK QTQVNKEMIT NFLIVLSFFA PYLAEELNSK 

       730        740        750        760        770        780 
LNNPTLLYKM RLAQWDEAYL VKNTTTISCS INGKFKLVHE FDLDSDEQEV ANYFLNQDLI 

       790        800 
KRNLENKKLV KTIFVKNKVI NFIIK 

« Hide

References

[1]"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015450 Genomic DNA. Translation: AAP56783.1.
RefSeqNP_853215.1. NC_004829.2.

3D structure databases

ProteinModelPortalQ7NB47.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233150.MGA_0087.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP56783; AAP56783; MGA_0087.
GeneID1089816.
KEGGmga:MGA_0087.
PATRIC20008713. VBIMycGal115357_0491.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycMGAL710127:GC09-501-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MYCGA
AccessionPrimary (citable) accession number: Q7NB47
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries