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Protein

Ribonuclease 3

Gene

rnc

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi472 – 4721MagnesiumBy similarity
Active sitei476 – 4761Sequence Analysis
Metal bindingi542 – 5421MagnesiumBy similarity
Active sitei545 – 5451By similarity
Metal bindingi545 – 5451MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciMGAL710127:GC09-561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:rnc
Ordered Locus Names:MYCGA4930
ORF Names:MGA_0180
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655Ribonuclease 3PRO_0000416608Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini432 – 556125RNase IIIAdd
BLAST
Domaini582 – 64968DRBMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 400400UnknownAdd
BLAST
Regioni401 – 655255RNase 3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi92 – 380289Asn-richAdd
BLAST

Sequence similaritiesi

Contains 1 RNase III domain.Curated

Phylogenomic databases

KOiK03685.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7NAZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENLEKNTKK KIKKPNNFKK NNKDKTEELK DKPTPRMFKM TPKSSKFLHQ
60 70 80 90 100
LGVTGRKHTE KPVPLNDPDY EKKIREIQAK EAKKIRSDEV VNNNSKKQNN
110 120 130 140 150
NKQAKKKANK NKKQKGNNNN FQNNKKPENW KQKPAANNQV KAIPNSEKST
160 170 180 190 200
AKTAINLIIK RTFETLKQNN LIAPNLKQNP NKKDKPQQPN VAAKNNNQKE
210 220 230 240 250
VKKPYNNFVN NQKNHNKNNA GNKGDNKQPT KPLNQSKLSK STIVELPFTP
260 270 280 290 300
NFTSNQPKPT QKEDSKKVKA KKAENSQPQE SNKQVKKLEV KTNQQKQDQT
310 320 330 340 350
KKKQPKENKN QQIKAVNLNN NQQKTNNNNQ KNSVDKSEND NNKKKSEANQ
360 370 380 390 400
KQENLNPNNN NKKKEDSKNE SNNIPLINKN ISDQQIVKIS NYIKDNYPVI
410 420 430 440 450
YADLKEKNRL GFNSNLDDDK LIVYANYEAK DLELLLKKFK VVTNNIGLYE
460 470 480 490 500
EALTHNSYAN EMHLKYNYQR LEFLGDAIIN KIVAEYLFNH SDSSEGEMTK
510 520 530 540 550
DRIKIIQSNT LIKAATQLEL INYIRVGEGL KIAPLSPKIL EDIFEAFIGA
560 570 580 590 600
MYLDQGEYAV RKILNDTIIG YYQKGQLTEN TDYKSIFQEI IHSTGLNMKI
610 620 630 640 650
HYERTYDRQK NLHTVSLYAG GIMYGEGKDS STHKAEIKAA KEAISKFRGL

LKLEK
Length:655
Mass (Da):75,387
Last modified:March 23, 2010 - v2
Checksum:iBABBEDB7BAF8E4BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015450 Genomic DNA. Translation: AAP56843.2.
RefSeqiWP_011113742.1. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56843; AAP56843; MGA_0180.
GeneIDi1089756.
KEGGimga:MGA_0180.
PATRICi20008837. VBIMycGal115357_0551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015450 Genomic DNA. Translation: AAP56843.2.
RefSeqiWP_011113742.1. NC_004829.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP56843; AAP56843; MGA_0180.
GeneIDi1089756.
KEGGimga:MGA_0180.
PATRICi20008837. VBIMycGal115357_0551.

Phylogenomic databases

KOiK03685.

Enzyme and pathway databases

BioCyciMGAL710127:GC09-561-MONOMER.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
    Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
    Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R(low / passage 15 / clone 2).

Entry informationi

Entry nameiRNC_MYCGA
AccessioniPrimary (citable) accession number: Q7NAZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 23, 2010
Last modified: July 22, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.