Q7NAM0 (ASNA_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	MYCGA6160
ORF Names:	MGA_0424

Organism

Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]

Taxonomic identifier

710127 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195882

Sequences

	Sequence		Length	Mass (Da)
	Q7NAM0 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: 300C80E0A974789A	FASTA	327	37,760
	10 20 30 40 50 60 MKKLTLLETE LAIKYIKDLF QNALSKELNL LRVSAPLIIA PDSGLNDNLN GWEAPVSFKS 70 80 90 100 110 120 KTNGVSSQVV QSLAKWKRYS IARYEIPLYQ GLYTDMNAIR MDETLDATHS MYVDQWDWEL 130 140 150 160 170 180 RISENDRNVE FLKNTVNKIY KVLKEAQLKV NEKYGIFEQK DLLPEHIHFV TTQELLDQYP 190 200 210 220 230 240 DKNPSEREQL VCEKYKAVFV MQVGKKLSNN QVHDGRSPDY DDWSLNGDIM VYNPRSKKAL 250 260 270 280 290 300 ELSSMGIRVN KEVLLKQLEE SKQNERLELM FHKKLVNGEL HQTIGGGIGQ SRLCYFLLQK 310 320 DHIGEVQASH WSDEIVVEAK AKGIKLL

References

[1]

"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)." Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J. Microbiology 149:2307-2316(2003) [PubMed: 12949158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015450 Genomic DNA. Translation: AAP56966.2.
RefSeq	NP_853398.2. NC_004829.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1089969.
GenomeReviews	Gene locus MYCGA6160 in contig AE015450_GR.
KEGG	mga:MGA_0424.
PATRIC	20009125. VBIMycGal115357_0693.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
PhylomeDB	Q7NAM0.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_MYCGA

Accession

Primary (citable) accession number: Q7NAM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 15, 2010
Last modified:	January 25, 2012
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program