Reviewed, UniProtKB/Swiss-Prot Q7NAM0 (ASNA_MYCGA)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	MYCGA6160
ORF Names:	MGA_0424

Organism

Mycoplasma gallisepticum [Complete proteome] [HAMAP]

Taxonomic identifier

2096 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195882

Sequences

	Sequence		Length	Mass (Da)
	Q7NAM0-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 5B7693E6C1758E40	FASTA	329	38,005
	10 20 30 40 50 60 MIMKKLTLLE TELAIKYIKD LFQNALSKEL NLLRVSAPLI IAPDSGLNDN LNGWEAPVSF 70 80 90 100 110 120 KSKTNGVSSQ VVQSLAKWKR YSIARYEIPL YQGLYTDMNA IRMDETLDAT HSMYVDQWDW 130 140 150 160 170 180 ELRISENDRN VEFLKNTVNK IYKVLKEAQL KVNEKYGIFE QKDLLPEHIH FVTTQELLDQ 190 200 210 220 230 240 YPDKNPSERE QLVCEKYKAV FVMQVGKKLS NNQVHDGRSP DYDDWSLNGD IMVYNPRSKK 250 260 270 280 290 300 ALELSSMGIR VNKEVLLKQL EESKQNERLE LMFHKKLVNG ELHQTIGGGI GQSRLCYFLL 310 320 QKDHIGEVQA SHWSDEIVVE AKAKGIKLL

References

[1]

"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)." Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J. Microbiology 149:2307-2316(2003) [PubMed: 12949158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015450 Genomic DNA. Translation: AAP56966.1.
RefSeq	NP_853398.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1089969.
GenomeReviews	Gene locus MYCGA6160 in contig AE015450_GR.
KEGG	mga:MGA_0424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7NAM0.
OMA	LNDNLNG.
Enzyme and pathway databases
BRENDA	6.3.1.1. 141882.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_MYCGA

Accession

Primary (citable) accession number: Q7NAM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)