Q7NAE5 (SYE_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	MYCGA7130
ORF Names:	MGA_0594

Organism

Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]

Taxonomic identifier

710127 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119600
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7NAE5 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: D9643C717B49D8C8	FASTA	498	57,981
	10 20 30 40 50 60 MSKIRTRYAP SPTGYFHIGG ARTALFNYLF AKHNNGEFIV RIEDTDIERN VEDGAENQLY 70 80 90 100 110 120 NLKWLNIFAD ESIWNPTQSG PYRQSEKLEV YQKYAYQLLE EKKAYRCFCT PEELQKHRED 130 140 150 160 170 180 LLKQYKPPIY SRKCFLLSEE QIHKNLADKI PFTIRLLLRD NKEYSWNDLI RGNLIFNTSS 190 200 210 220 230 240 MSDPVILKSN QIATYNFAVV IDDHDMKISH ILRGEEHISN TPYQLAIKEA LGFKDEFVYG 250 260 270 280 290 300 HLSIIVDETG KKLSKRNLAV EQFVEGFRKK GYLAEALVNF IALLGWSHPD NIEILDLPTL 310 320 330 340 350 360 VKSFTIKNLS AAPSFFDIKK LNWISSEYIK NMDDVMYLAF IKPYVDLNEY DEIKTKVNEI 370 380 390 400 410 420 CLMFKNQLQY GYQINEIIKE SFVPHVGLSN LDQADLDFLK SNPNYRQLLI SFKEKINQLD 430 440 450 460 470 480 QVNDHNVKEI INWLAHQIKL GDLVLEKPLG GKNLYMPLRI VLSNKKHGPE LNKVIALYDK 490 QTILKNLDDA INYLTNAK

References

[1]

"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)." Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J. Microbiology 149:2307-2316(2003) [PubMed: 12949158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015450 Genomic DNA. Translation: AAP57063.1.
RefSeq	NP_853495.1. NC_004829.2.
3D structure databases
ProteinModelPortal	Q7NAE5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1090315.
GenomeReviews	Gene locus MYCGA7130 in contig AE015450_GR.
KEGG	mga:MGA_0594.
PATRIC	20009349. VBIMycGal115357_0803.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	SVKWLLN.
PhylomeDB	Q7NAE5.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MYCGA

Accession

Primary (citable) accession number: Q7NAE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program