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Q7NAE5 (SYE_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:MYCGA7130
ORF Names:MGA_0594
OrganismMycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]
Taxonomic identifier710127 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119600

Regions

Motif10 – 2011"HIGH" region HAMAP-Rule MF_00022
Motif252 – 2565"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2551ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7NAE5 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: D9643C717B49D8C8

FASTA49857,981
        10         20         30         40         50         60 
MSKIRTRYAP SPTGYFHIGG ARTALFNYLF AKHNNGEFIV RIEDTDIERN VEDGAENQLY 

        70         80         90        100        110        120 
NLKWLNIFAD ESIWNPTQSG PYRQSEKLEV YQKYAYQLLE EKKAYRCFCT PEELQKHRED 

       130        140        150        160        170        180 
LLKQYKPPIY SRKCFLLSEE QIHKNLADKI PFTIRLLLRD NKEYSWNDLI RGNLIFNTSS 

       190        200        210        220        230        240 
MSDPVILKSN QIATYNFAVV IDDHDMKISH ILRGEEHISN TPYQLAIKEA LGFKDEFVYG 

       250        260        270        280        290        300 
HLSIIVDETG KKLSKRNLAV EQFVEGFRKK GYLAEALVNF IALLGWSHPD NIEILDLPTL 

       310        320        330        340        350        360 
VKSFTIKNLS AAPSFFDIKK LNWISSEYIK NMDDVMYLAF IKPYVDLNEY DEIKTKVNEI 

       370        380        390        400        410        420 
CLMFKNQLQY GYQINEIIKE SFVPHVGLSN LDQADLDFLK SNPNYRQLLI SFKEKINQLD 

       430        440        450        460        470        480 
QVNDHNVKEI INWLAHQIKL GDLVLEKPLG GKNLYMPLRI VLSNKKHGPE LNKVIALYDK 

       490 
QTILKNLDDA INYLTNAK 

« Hide

References

[1]"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015450 Genomic DNA. Translation: AAP57063.1.
RefSeqNP_853495.1. NC_004829.2.

3D structure databases

ProteinModelPortalQ7NAE5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233150.MGA_0594.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP57063; AAP57063; MGA_0594.
GeneID1090315.
KEGGmga:MGA_0594.
PATRIC20009349. VBIMycGal115357_0803.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAWLPEEMG.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycMGAL710127:GC09-804-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_MYCGA
AccessionPrimary (citable) accession number: Q7NAE5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries