Reviewed, UniProtKB/Swiss-Prot Q7NA83 (ASNA_PHOLL)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	plu0052

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195885

Sequences

	Sequence		Length	Mass (Da)
	Q7NA83-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 6BAAB2572ADC497D	FASTA	330	37,296
	10 20 30 40 50 60 MKKSFIEKQQ QISFVKSFFS RLLESKLGLI EVQGPILSCL GDGIQDNLSG HEKAVQVKVK 70 80 90 100 110 120 TLPDSTFEVV HSLAKWKRRT LGRFGFQPEQ GLYTHMKALR PDEDRLTPIH SVYVDQWDWE 130 140 150 160 170 180 KVMEDGQRSL AYLKQTVGKI YEAIKETEKA VNKEFGLAPF LPDQIHFVHS ETLLSRYPDL 190 200 210 220 230 240 DAKGRERAIA KELGAVFLIG IGAKLSDGQS HDVRAPDYDD WTTPNSDGFV GLNGDIIVWN 250 260 270 280 290 300 PVLEDAFEIS SMGIRVDAEA LERQLALTSD EERLQYDWHQ ALLKGDMPQS IGGGIGQSRL 310 320 330 VMLLLQMKHI GQVQCSIWSP EVRETVEDML

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571859 Genomic DNA. Translation: CAE12347.1.
RefSeq	NP_927428.1.
3D structure databases
SMR	Q7NA83. Positions 4-330.
ModBase	Search...
Genome annotation databases
GeneID	2799996.
KEGG	plu:plu0052.
NMPDR	fig|243265.1.peg.52.
Organism-specific databases
PhotoList	plu0052.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU0052-MONOMER.
BRENDA	6.3.1.1. 308689.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. PF00152. tRNA-synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_PHOLL

Accession

Primary (citable) accession number: Q7NA83

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 15, 2003
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)