Reviewed, UniProtKB/Swiss-Prot Q7N9W0 (SYY2_PHOLL)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase 2
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase 2
      Short name=TyrRS 2

Gene names

Name:	tyrS2
Ordered Locus Names:	plu0201

Organism

Photorhabdus luminescens subsp. laumondii [Complete proteome] [HAMAP]

Taxonomic identifier

141679 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Tyrosyl-tRNA synthetase 2 HAMAP MF_02007		PRO_0000236743
Regions
	Domain	338 – 398	61	S4 RNA-binding
	Motif	43 – 52	10	"HIGH" region HAMAP MF_02007
	Motif	227 – 231	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	230	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N9W0-1 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: 40F1C9E90A4E57E6	FASTA	399	45,104
	10 20 30 40 50 60 MKENIDKLLS NIAIMEPPLG LQDKLALARQ ENRKLTIKLG FDPTAPDLHL GHAVVLQKLK 70 80 90 100 110 120 DFQDEGHRIV VIIGDFTAGI GDPTGRNKLR PPLTPEQINK NSQTYINQLA KVINIENIEI 130 140 150 160 170 180 RKNSEWFNNM PFSNVIKLIS KITLAQIMHR DDFKTRFESK APVHLHEIIY PILQGYDSVM 190 200 210 220 230 240 IDADIELGGT DQLFNNLVGR TLQEAYEKKG QIVITMPLLE GLDGIEKMSK SKNNYIGLTD 250 260 270 280 290 300 NANDMYGKVM SIPDSVIINY LTLATDMEAE KQSAIVSQLE LGLNPMKIKK DIAYNIVKRY 310 320 330 340 350 360 HDDISAKEAT EHFERVVQKR TPEEADHDVL ILPKGSYITL LDLCSVALPA ISRSELRRLI 370 380 390 RSGAVRVDKS KEDDEIKNIE VIPGTLIWIG KRYKFRIGS

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed: 14528314] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571859 Genomic DNA. Translation: CAE12496.1.
RefSeq	NP_927565.1.
3D structure databases
HSSP	HSSP built from PDB template 1H3F based on UniProtKB P83453.
ModBase	Search...
Genome annotation databases
GeneID	2800150.
GenomeReviews	Gene locus plu0201 in contig BX470251_GR.
KEGG	plu:plu0201.
NMPDR	fig|243265.1.peg.189.
Organism-specific databases
PhotoList	plu0201.
CMR	Search...
Phylogenomic databases
HOGENOM	Q7N9W0.
OMA	INADIEM.
Enzyme and pathway databases
BioCyc	PLUM243265:PLU0201-MON.
BRENDA	6.1.1.1. 308689.
Family and domain databases
HAMAP	MF_02007. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY2_PHOLL

Accession

Primary (citable) accession number: Q7N9W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	December 15, 2003
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)