Q7N9V7 (BIOH_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pimelyl-[acyl-carrier protein] methyl ester esterase
EC=3.1.1.85

Alternative name(s):
Biotin synthesis protein BioH
Carboxylesterase BioH

Gene names

Name:	bioH
Ordered Locus Names:	plu0204

Organism

Photorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]

Taxonomic identifier

243265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus › Photorhabdus luminescens › Photorhabdus luminescens subsp. laumondii

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters By similarity. HAMAP-Rule MF_01260
Catalytic activity	Pimelyl-[acyl-carrier protein] methyl ester + H2O = pimelyl-[acyl-carrier protein] + methanol. HAMAP-Rule MF_01260
Pathway	Cofactor biosynthesis; biotin biosynthesis. HAMAP-Rule MF_01260
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carboxylesterase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Pimelyl-[acyl-carrier protein] methyl ester esterase HAMAP-Rule MF_01260		PRO_0000204486
Regions
	Region	82 – 83	2	Substrate binding By similarity
	Region	143 – 147	5	Substrate binding By similarity
Sites
	Active site	82	1	Nucleophile By similarity
	Active site	207	1	By similarity
	Active site	235	1	By similarity
	Binding site	22	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	235	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7N9V7 [UniParc]. Last modified December 15, 2003. Version 1. Checksum: A010A29E2A51B77A	FASTA	261	29,519
	10 20 30 40 50 60 MADLFWQTSD EGSRDLVLLH GWGLNAEVWR SIEMRCAPHF RLHLVDLPGY GRSQKYGPMN 70 80 90 100 110 120 LADMADEVWR YAPENALWLG WSLGGLVASR IALDHQDKVA GLITVASSPH FSAESDWPGI 130 140 150 160 170 180 KPEVLHDFEH QLSKDFQRTV ERFLALQTLG TDSARQDARL LKSVVLAQPM PSVEVLHVGL 190 200 210 220 230 240 EILRTEDLRQ PLAELAIPFL RIYGYLDGLV PRKIINILDE KWPRSVSAMM RHAAHAPFIS 250 260 HPDEFVGLLT EFASREVLQG R

References

[1]

"The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens." Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F., Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C., Lanois A., Powell K. , Siguier P., Vincent R., Wingate V., Zouine M., Glaser P., Boemare N., Danchin A., Kunst F. Nat. Biotechnol. 21:1307-1313(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TT01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571859 Genomic DNA. Translation: CAE12499.1.
RefSeq	NP_927568.1. NC_005126.1.
3D structure databases
ProteinModelPortal	Q7N9V7.
ModBase	Search...
Protein-protein interaction databases
STRING	243265.plu0204.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE12499; CAE12499; plu0204.
GeneID	2800153.
KEGG	plu:plu0204.
PATRIC	20503863. VBIPhoLum48522_0216.
Organism-specific databases
GenoList	plu0204.
CMR	Search...
Phylogenomic databases
eggNOG	COG0596.
HOGENOM	HOG000028062.
KO	K02170.
OMA	GIKSFLH.
ProtClustDB	CLSK2521647.
Enzyme and pathway databases
UniPathway	UPA00078.
Family and domain databases
HAMAP	MF_01260. Carboxylester.
InterPro	IPR010076. BioH. [Graphical view]
TIGRFAMs	TIGR01738. bioH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOH_PHOLL

Accession

Primary (citable) accession number: Q7N9V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	December 15, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program