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Q7N9V7 (BIOH_PHOLL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esterase

EC=3.1.1.85
Alternative name(s):
Biotin synthesis protein BioH
Carboxylesterase BioH
Gene names
Name:bioH
Ordered Locus Names:plu0204
OrganismPhotorhabdus luminescens subsp. laumondii (strain TT01) [Complete proteome] [HAMAP]
Taxonomic identifier243265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters By similarity. HAMAP-Rule MF_01260

Catalytic activity

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol. HAMAP-Rule MF_01260

Pathway

Cofactor biosynthesis; biotin biosynthesis. HAMAP-Rule MF_01260

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01260

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01260.

Sequence similarities

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   Molecular functionHydrolase
Serine esterase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarboxylic ester hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261Pimeloyl-[acyl-carrier protein] methyl ester esterase HAMAP-Rule MF_01260
PRO_0000204486

Regions

Region82 – 832Substrate binding By similarity
Region143 – 1475Substrate binding By similarity

Sites

Active site821Nucleophile By similarity
Active site2071 By similarity
Active site2351 By similarity
Binding site221Substrate; via amide nitrogen and carbonyl oxygen By similarity
Binding site2351Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7N9V7 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: A010A29E2A51B77A

FASTA26129,519
        10         20         30         40         50         60 
MADLFWQTSD EGSRDLVLLH GWGLNAEVWR SIEMRCAPHF RLHLVDLPGY GRSQKYGPMN 

        70         80         90        100        110        120 
LADMADEVWR YAPENALWLG WSLGGLVASR IALDHQDKVA GLITVASSPH FSAESDWPGI 

       130        140        150        160        170        180 
KPEVLHDFEH QLSKDFQRTV ERFLALQTLG TDSARQDARL LKSVVLAQPM PSVEVLHVGL 

       190        200        210        220        230        240 
EILRTEDLRQ PLAELAIPFL RIYGYLDGLV PRKIINILDE KWPRSVSAMM RHAAHAPFIS 

       250        260 
HPDEFVGLLT EFASREVLQG R 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX571859 Genomic DNA. Translation: CAE12499.1.
RefSeqNP_927568.1. NC_005126.1.

3D structure databases

ProteinModelPortalQ7N9V7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243265.plu0204.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE12499; CAE12499; plu0204.
GeneID2800153.
KEGGplu:plu0204.
PATRIC20503863. VBIPhoLum48522_0216.

Organism-specific databases

GenoListplu0204.
CMRSearch...

Phylogenomic databases

eggNOGCOG0596.
HOGENOMHOG000028062.
KOK02170.
OMAPATWPVS.
OrthoDBEOG6FJNH9.
ProtClustDBCLSK2521647.

Enzyme and pathway databases

UniPathwayUPA00078.

Family and domain databases

HAMAPMF_01260. Carboxylester.
InterProIPR010076. BioH.
[Graphical view]
TIGRFAMsTIGR01738. bioH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOH_PHOLL
AccessionPrimary (citable) accession number: Q7N9V7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 15, 2003
Last modified: February 19, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways