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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Photorhabdus luminescens subsp. laumondii (strain TT01)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathway:ibiotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei82 – 821NucleophileUniRule annotation
Active sitei207 – 2071UniRule annotation
Active sitei235 – 2351UniRule annotation
Binding sitei235 – 2351SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00078.

Protein family/group databases

ESTHERipholu-BIOH. BioH.
MEROPSiS33.994.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:plu0204
OrganismiPhotorhabdus luminescens subsp. laumondii (strain TT01)
Taxonomic identifieri243265 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus
ProteomesiUP000002514 Componenti: Chromosome

Organism-specific databases

GenoListiplu0204.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_0000204486Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243265.plu0204.

Structurei

3D structure databases

ProteinModelPortaliQ7N9V7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate bindingUniRule annotation
Regioni143 – 1475Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028062.
KOiK02170.
OMAiTIGEGDC.
OrthoDBiEOG6FJNH9.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7N9V7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLFWQTSD EGSRDLVLLH GWGLNAEVWR SIEMRCAPHF RLHLVDLPGY
60 70 80 90 100
GRSQKYGPMN LADMADEVWR YAPENALWLG WSLGGLVASR IALDHQDKVA
110 120 130 140 150
GLITVASSPH FSAESDWPGI KPEVLHDFEH QLSKDFQRTV ERFLALQTLG
160 170 180 190 200
TDSARQDARL LKSVVLAQPM PSVEVLHVGL EILRTEDLRQ PLAELAIPFL
210 220 230 240 250
RIYGYLDGLV PRKIINILDE KWPRSVSAMM RHAAHAPFIS HPDEFVGLLT
260
EFASREVLQG R
Length:261
Mass (Da):29,519
Last modified:December 15, 2003 - v1
Checksum:iA010A29E2A51B77A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571859 Genomic DNA. Translation: CAE12499.1.
RefSeqiWP_011144604.1. NC_005126.1.

Genome annotation databases

EnsemblBacteriaiCAE12499; CAE12499; plu0204.
GeneIDi24168446.
KEGGiplu:plu0204.
PATRICi20503863. VBIPhoLum48522_0216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571859 Genomic DNA. Translation: CAE12499.1.
RefSeqiWP_011144604.1. NC_005126.1.

3D structure databases

ProteinModelPortaliQ7N9V7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243265.plu0204.

Protein family/group databases

ESTHERipholu-BIOH. BioH.
MEROPSiS33.994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE12499; CAE12499; plu0204.
GeneIDi24168446.
KEGGiplu:plu0204.
PATRICi20503863. VBIPhoLum48522_0216.

Organism-specific databases

GenoListiplu0204.

Phylogenomic databases

eggNOGiCOG0596.
HOGENOMiHOG000028062.
KOiK02170.
OMAiTIGEGDC.
OrthoDBiEOG6FJNH9.

Enzyme and pathway databases

UniPathwayiUPA00078.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TT01.

Entry informationi

Entry nameiBIOH_PHOLL
AccessioniPrimary (citable) accession number: Q7N9V7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 15, 2003
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.